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    v1.0.30
    OpenPayerBilling CodesReproductive Medicine ProceduresCPT cpt-89280-oocyte-microtechnique-10-or-fewer
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    CPT 89280: Oocyte Fertilization Using Microtechniques (≤10 oocytes)

    CPT code 89280 describes laboratory technical procedures where a clinical lab analyst assists in fertilizing oocytes using microtechniques for cycles involving 10 or fewer oocytes; the service is a laboratory-based assisted reproductive technology procedure (technical component) typically performed in an embryology or assisted reproduction laboratory within a fertility clinic or specialized reproductive medicine center.

    For related coverage guidance, see recent payer policy updates: Prenatal (Fetal) Diagnostic Testing, Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency.

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    Clinical & Coding Specifications

    Clinical Context

    A 34-year-old patient undergoing an assisted reproductive technology cycle presents to the embryology laboratory on the day of oocyte retrieval. The reproductive endocrinology team has performed transvaginal ultrasound-guided follicular aspiration and delivered the retrieved oocytes to the embryology lab. The laboratory analyst performs microtechniques to assist fertilization of up to ten oocytes, such as intracytoplasmic sperm injection (ICSI) preparation steps, micromanipulation, and immediate technical handling required to place sperm into or near the oocyte. The workflow includes verifying patient and specimen identifiers, assessing oocyte maturity, preparing sperm (if not already prepared), performing micromanipulation under inverted microscopy, documenting technical steps and outcomes, and transferring inseminated oocytes to appropriate culture media for incubation and monitoring. Typical site of service is an outpatient hospital-based or freestanding outpatient fertility clinic embryology laboratory. The patient scenario commonly involves an infertility diagnosis such as male factor infertility, prior fertilization failure, or planned use of assisted fertilization techniques during an in vitro fertilization cycle.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    58970Oocyte retrieval, any method; transvaginal ultrasound guidancePerformed immediately prior to embryology microtechniques; provides the oocytes that are processed under 89280.
    89250Gamete, sperm processing for assisted reproductive procedures (eg, for intrauterine insemination)Sperm preparation that commonly occurs before or concurrent with microtechniques such as ICSI.
    89281Embryo culture, less than 4 days, with observation and documentationSubsequent laboratory incubation and monitoring of fertilized oocytes following the microtechnique procedure.
    89342Assisted embryo hatching, any methodA related embryology service that may be performed later in the IVF cycle for selected embryos.
    58300Insertion of an intrauterine device (IUD)Example of a concurrent gynecologic procedure sometimes billed during fertility care cycles (procedural context), though not directly part of microfertilization.
    89279Oocyte identification and assessment, nonmicromanipulation techniquesAlternative or preceding lab assessment of oocytes when micromanipulation is not performed.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    55Modifier –55 indicates the billing provider furnished only postoperative management for a procedure that has a global surgical period. According to AAPC, this modifier is appended to the same CPT…- Use modifier -55 when billing the same CPT surgical code that has a 10- or 90-day global period and you, as a provider other than the original surgeon, furnished ONLY postoperative management (no…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    213E00000X
    363A00000X
    207L00000X
    367A00000X
    224P00000X
    261QA3000X
    101YS0200X
    207U00000X

    Related ICD-10-CM Codes

    22 rows

    ICD-10-CM CodeDescription
    D28.9Benign neoplasm of female genital organ, unspecified
    E03.9Hypothyroidism, unspecified
    E16.2Hypoglycemia, unspecified
    E23.0Hypopituitarism
    E28.0Estrogen excess
    E28.2Polycystic ovarian syndrome
    E28.9Ovarian dysfunction, unspecified
    E29.1Testicular hypofunction
    N91.2Amenorrhea, unspecified
    N97.0Female infertility associated with anovulation

    Showing 1–10 of 22

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Prenatal (Fetal) Diagnostic TestingBlue Cross Blue Shield - Rhode Island2027-01-01
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Polygenic Risk ScoreBlue Cross Blue Shield - Rhode Island2026-12-01
    Gene Expression Profiling for Cutaneous MelanomaBlue Cross Blue Shield - Rhode Island2026-12-01
    Genetic Testing for Diagnosis and Management of Mental Health ConditionsBlue Cross Blue Shield - Rhode Island2026-11-01
    Molecular Testing in the Management of Pulmonary NodulesBlue Cross Blue Shield - Rhode Island2026-11-01
    Genetic Testing for Hereditary Cancer (for North Carolina Only)Colorado Rocky Mountain Health Plans2026-10-01
    Expanded Molecular Testing of Cancers to Select Targeted TherapiesBlue Cross Blue Shield - North Dakota2026-10-01

    Related Codes

    CPT-89250-SHORT-TERM-OOCYTE-EMBRYO-CULTURE-ART

    CPT 89250: Oocyte or Embryo Culture <4 Days

    CPT-89251-OOCYTE-EMBRYO-SHORT-TERM-COCULTURE-ART

    CPT 89251: Oocyte/Embryo Culture with Coculture (<4 days)

    CPT-89253-ASSISTED-EMBRYO-HATCHING-PARTIAL-ZONA-DISSECTION

    CPT 89253: Assisted Embryo Hatching, Partial Zona Dissection

    CPT-89254-FOLLICULAR-FLUID-OOCYTE-IDENTIFICATION-LAB

    CPT 89254: Oocyte Identification from Follicular Fluid

    CPT-89255-EMBRYO-PREPARATION-FOR-TRANSFER-ART

    CPT 89255: Embryo Preparation for Transfer (ART)

    CPT-89257-SPERM-IDENTIFICATION-FROM-ASPIRATION

    CPT 89257: Identification of Sperm from Non-Seminal Aspirates

    CPT-89258-EMBRYO-CRYOPRESERVATION-IVF

    CPT 89258: Embryo Cryopreservation During IVF Cycle

    CPT-89259-SPERM-CRYOPRESERVATION-AND-PRESERVATION

    CPT 89259: Sperm Cryopreservation, Laboratory Processing

    CPT-89260-LABORATORY-SPERM-PREPARATION-SPERM-WASH-SWIM-UP

    CPT 89260: Sperm Isolation for Sperm Wash and Swim‑Up Preparation

    CPT-89261-SPERM-PREPARATION-AND-SEMEN-ANALYSIS

    CPT 89261: Complex Sperm Preparation and Semen Analysis

    Showing 1–10 of 36

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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