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    v1.0.30
    OpenPayerBilling CodesReproductive Medicine ProceduresCPT cpt-89259-sperm-cryopreservation-and-preservation
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    CPT 89259: Sperm Cryopreservation, Laboratory Processing

    CPT code 89259 describes laboratory cryopreservation of sperm performed by a lab analyst, a sperm cryopreservation service used to preserve male reproductive cells for future use; the service is a laboratory procedure typically performed in an andrology or fertility clinic laboratory setting and involves processing and freezing sperm specimens for long-term storage.

    For related coverage guidance, see recent payer policy updates: Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency, Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policy.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 32-year-old male presents to an andrology laboratory for sperm cryopreservation prior to planned gonadotoxic therapy for Hodgkin lymphoma. The patient undergoes a baseline semen analysis followed by collection via masturbation in a private collection room. The lab analyst documents specimen identification, performs semen parameter assessment, mixes sample with an appropriate cryoprotectant, aliquots into labeled cryovials or straws, follows a controlled-rate freezing protocol, and transfers vials into long-term storage in liquid nitrogen tanks. Chain-of-custody, patient consent for storage and future use, and labeling requirements are completed. The service is billed using 89259 for the lab analyst’s cryopreservation procedure; typically the site of service is an outpatient fertility or hospital-based andrology/laboratory suite where tissue banking is performed.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    89300Spermatozoa; presence and quantitation/seminal analysis, non-automatedOften performed before 89259 to assess semen parameters guiding cryopreservation decisions.
    89320Sperm preparation with cell washing, simple wash or swim-up, each specimenPerformed when preparing sperm for freezing to remove seminal plasma or debris prior to cryoprotectant addition.
    89250Cryopreservation, embryo; per cryopreservation procedureRelated laboratory cryopreservation procedures for embryos; similar lab processes and facility resources (contextual relation).
    89258Post-thaw sperm viability testingPerformed after storage or thawing to evaluate viability following cryopreservation performed under 89259.
    89260Storage of reproductive tissue, per specimen, short- or long-term (e.g., sperm banking)Billed for ongoing storage fees associated with specimens cryopreserved under 89259.
    58322Treatment of incomplete abortion — uterine aspiration (example of unrelated OR procedures)Data not available in the input.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    106E00000X
    207RC0000X
    363A00000X
    246ZB0301X
    246R00000X
    101Y00000X
    207XX0004X
    225500000X

    Related ICD-10-CM Codes

    24 rows

    ICD-10-CM CodeDescription
    A56.09Other chlamydial infection of lower genitourinary tract
    A64Unspecified sexually transmitted disease
    C61Malignant neoplasm of prostate
    D56.9Thalassemia, unspecified
    E29.1Testicular hypofunction
    E29.8Other testicular dysfunction
    E29.9Testicular dysfunction, unspecified
    N40.0Benign prostatic hyperplasia without lower urinary tract symptoms
    N43.3Hydrocele, unspecified
    N46.9Male infertility, unspecified

    Showing 1–10 of 24

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Carrier Testing Panels for Genetic Diseases (for Kentucky Only)UnitedHealthcare2026-10-01
    Genetic Testing for Neurological Disorders (for Kansas Only)UnitedHealthcare2026-10-01
    Genetic and Molecular TestingProvidence Health Plan2026-10-01
    Cell-Free Fetal DNA Testing (for Idaho Only)UnitedHealthcare2026-10-01
    Next Generation Sequencing for Minimal Residual Disease DetectionProvidence Health Plan2026-10-01
    Pharmacogenetic Panel Testing (for Kansas Only)UnitedHealthcare2026-10-01
    Non-Covered Genetic Panel TestsProvidence Health Plan2026-10-01

    Related Codes

    CPT-89250-SHORT-TERM-OOCYTE-EMBRYO-CULTURE-ART

    CPT 89250: Oocyte or Embryo Culture <4 Days

    CPT-89251-OOCYTE-EMBRYO-SHORT-TERM-COCULTURE-ART

    CPT 89251: Oocyte/Embryo Culture with Coculture (<4 days)

    CPT-89253-ASSISTED-EMBRYO-HATCHING-PARTIAL-ZONA-DISSECTION

    CPT 89253: Assisted Embryo Hatching, Partial Zona Dissection

    CPT-89254-FOLLICULAR-FLUID-OOCYTE-IDENTIFICATION-LAB

    CPT 89254: Oocyte Identification from Follicular Fluid

    CPT-89255-EMBRYO-PREPARATION-FOR-TRANSFER-ART

    CPT 89255: Embryo Preparation for Transfer (ART)

    CPT-89257-SPERM-IDENTIFICATION-FROM-ASPIRATION

    CPT 89257: Identification of Sperm from Non-Seminal Aspirates

    CPT-89258-EMBRYO-CRYOPRESERVATION-IVF

    CPT 89258: Embryo Cryopreservation During IVF Cycle

    CPT-89260-LABORATORY-SPERM-PREPARATION-SPERM-WASH-SWIM-UP

    CPT 89260: Sperm Isolation for Sperm Wash and Swim‑Up Preparation

    CPT-89261-SPERM-PREPARATION-AND-SEMEN-ANALYSIS

    CPT 89261: Complex Sperm Preparation and Semen Analysis

    CPT-89264-SPERM-IDENTIFICATION-TESTICULAR-TISSUE

    CPT 89264: Identification of Sperm from Testicular Tissue

    Showing 1–10 of 36

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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