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    v1.0.30
    OpenPayerBilling CodesReproductive Medicine ProceduresCPT cpt-89254-follicular-fluid-oocyte-identification-lab
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    CPT 89254: Oocyte Identification from Follicular Fluid

    CPT code 89254 describes a laboratory technical procedure in which a lab analyst examines follicular fluid to identify and report the presence of oocytes (eggs); the service type is a microscopic reproductive laboratory analysis and the typical site of service is an assisted reproductive technology laboratory or fertility clinic lab.

    For related coverage guidance, see recent payer policy updates: Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency, Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policy.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 33-year-old woman undergoing in vitro fertilization (IVF) presents on the day of oocyte retrieval. Following transvaginal ultrasound-guided follicular aspiration performed by the reproductive endocrinologist, follicular fluid samples are immediately transported to the embryology laboratory. The lab analyst performs 89254 by examining the aspirated follicular fluid under a stereomicroscope to identify and confirm the presence of oocytes (eggs). Identified oocytes are isolated, placed into appropriate culture media, and labeled for subsequent procedures such as oocyte assessment, intracytoplasmic sperm injection (ICSI), or conventional insemination. The technical workflow includes specimen accessioning, microscopic scanning of follicular fluid, documentation of oocyte number and quality, and communication of results to the clinical team for real-time decision-making in the IVF cycle.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    58970Oocyte retrieval, any technique (eg, transvaginal aspiration, surgical)Performed immediately before 89254; provides the follicular fluid specimen for oocyte identification

    59870 | (Not applicable) | Data not available in the input.

    89320 | Sperm evaluation; initial or comprehensive (qualitative and quantitative) | Performed in parallel in the IVF workflow for semen assessment when planning fertilization after oocyte identification

    89250 | In vitro fertilization; handling of oocytes and/or sperm for insemination procedures (note: code series related to embryology procedures) | Often sequenced in the lab workflow after 89254 when preparing oocytes for insemination or injection

    89260 | Assisted hatching of embryo, microtechnique | Occurs later in the embryology timeline after fertilization and embryo development following procedures that began with 89254

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    55Modifier –55 indicates the billing provider furnished only postoperative management for a procedure that has a global surgical period. According to AAPC, this modifier is appended to the same CPT…- Use modifier -55 when billing the same CPT surgical code that has a 10- or 90-day global period and you, as a provider other than the original surgeon, furnished ONLY postoperative management (no…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    163W00000X
    103K00000X
    3336C0002X
    207RC0000X
    363L00000X
    261QM1000X
    364SI0800X
    207UN0901X

    Related ICD-10-CM Codes

    20 rows

    ICD-10-CM CodeDescription
    E28.1Androgen excess
    E28.2Polycystic ovarian syndrome
    E28.8Other ovarian dysfunction
    E28.9Ovarian dysfunction, unspecified
    N84.0Polyp of corpus uteri
    N84.1Polyp of cervix uteri
    N91.0Primary amenorrhea
    N91.1Secondary amenorrhea
    N91.2Amenorrhea, unspecified
    N91.3Primary oligomenorrhea

    Showing 1–10 of 20

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Carrier Testing Panels for Genetic Diseases (for Kentucky Only)UnitedHealthcare2026-10-01
    Genetic Testing for Neurological Disorders (for Kansas Only)UnitedHealthcare2026-10-01
    Genetic and Molecular TestingProvidence Health Plan2026-10-01
    Cell-Free Fetal DNA Testing (for Idaho Only)UnitedHealthcare2026-10-01
    Next Generation Sequencing for Minimal Residual Disease DetectionProvidence Health Plan2026-10-01
    Pharmacogenetic Panel Testing (for Kansas Only)UnitedHealthcare2026-10-01
    Non-Covered Genetic Panel TestsProvidence Health Plan2026-10-01

    Related Codes

    CPT-89250-SHORT-TERM-OOCYTE-EMBRYO-CULTURE-ART

    CPT 89250: Oocyte or Embryo Culture <4 Days

    CPT-89251-OOCYTE-EMBRYO-SHORT-TERM-COCULTURE-ART

    CPT 89251: Oocyte/Embryo Culture with Coculture (<4 days)

    CPT-89253-ASSISTED-EMBRYO-HATCHING-PARTIAL-ZONA-DISSECTION

    CPT 89253: Assisted Embryo Hatching, Partial Zona Dissection

    CPT-89255-EMBRYO-PREPARATION-FOR-TRANSFER-ART

    CPT 89255: Embryo Preparation for Transfer (ART)

    CPT-89257-SPERM-IDENTIFICATION-FROM-ASPIRATION

    CPT 89257: Identification of Sperm from Non-Seminal Aspirates

    CPT-89258-EMBRYO-CRYOPRESERVATION-IVF

    CPT 89258: Embryo Cryopreservation During IVF Cycle

    CPT-89259-SPERM-CRYOPRESERVATION-AND-PRESERVATION

    CPT 89259: Sperm Cryopreservation, Laboratory Processing

    CPT-89260-LABORATORY-SPERM-PREPARATION-SPERM-WASH-SWIM-UP

    CPT 89260: Sperm Isolation for Sperm Wash and Swim‑Up Preparation

    CPT-89261-SPERM-PREPARATION-AND-SEMEN-ANALYSIS

    CPT 89261: Complex Sperm Preparation and Semen Analysis

    CPT-89264-SPERM-IDENTIFICATION-TESTICULAR-TISSUE

    CPT 89264: Identification of Sperm from Testicular Tissue

    Showing 1–10 of 36

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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