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    v1.0.30
    OpenPayerBilling CodesReproductive Medicine ProceduresCPT cpt-89258-embryo-cryopreservation-ivf
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    CPT 89258: Embryo Cryopreservation During IVF Cycle

    CPT code 89258 describes laboratory cryopreservation of one or more embryos retrieved during an in vitro fertilization (IVF) cycle; this service is a reproductive lab procedure performed by a clinical laboratory or embryology team and the typical site of service is an outpatient fertility clinic or assisted reproductive technology laboratory supporting IVF treatment.

    For related coverage guidance, see recent payer policy updates: Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency, Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policy.

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    Clinical & Coding Specifications

    Clinical Context

    A 34-year-old patient undergoing an in vitro fertilization (IVF) cycle elects to cryopreserve surplus embryos created during controlled ovarian stimulation, egg retrieval, and fertilization. Following oocyte retrieval and laboratory fertilization (conventional insemination or intracytoplasmic sperm injection), embryos are cultured to an appropriate stage (usually cleavage or blastocyst). The embryology laboratory performs assessment, grading, and selection of one or more embryos for immediate transfer and cryopreservation of the remaining embryos. Cryopreservation may be indicated for fertility preservation, to avoid multiple embryo transfers in a fresh cycle, or when a fresh embryo transfer is contraindicated for medical reasons. The laboratory workflow includes embryo evaluation, preparation of cryoprotectant solutions, controlled-rate freezing or vitrification protocols, labeling, documentation, and storage in liquid nitrogen tanks. The typical site of service is an assisted reproductive technology (ART) clinic’s embryology laboratory within an outpatient surgical center or specialized fertility clinic. Patients commonly present after a monitored ovarian stimulation and transvaginal oocyte retrieval; the laboratory analyst documents the number of embryos cryopreserved, identifiers, and storage location in the patient’s reproductive health record.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    58970Transvaginal oocyte retrieval, aspiration of oocyte(s)Performed prior to embryo creation; provides oocytes that are fertilized into embryos later cryopreserved.
    58974Oocyte cryopreservation, any methodAlternative or companion procedure when oocytes rather than embryos are cryopreserved for fertility preservation.
    88360Pathology: special staining (if applicable)May be used for adjunct laboratory diagnostics in reproductive pathology contexts (rare).
    89250Embryo culture; from fertilization to blastocyst (per embryo)Embryo culture and monitoring performed prior to selection and cryopreservation.
    89257Embryo biopsy for preimplantation genetic testing (PGT)Performed prior to cryopreservation when genetic testing is indicated; embryos may be biopsied then frozen.
    89337Assisted reproductive technology; thawing of embryos for transferThawing code used when previously cryopreserved embryos are later warmed for transfer or further use.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    333600000X
    163W00000X
    207L00000X
    2084P0005X
    2255A2300X
    163WP0000X
    207RA0201X
    284300000X

    Related ICD-10-CM Codes

    17 rows

    ICD-10-CM CodeDescription
    E28.2Polycystic ovarian syndrome
    E28.9Ovarian dysfunction, unspecified
    N91.0Primary amenorrhea
    N92.6Irregular menstruation, unspecified
    N97.0Female infertility associated with anovulation
    N97.1Female infertility of tubal origin
    N97.2Female infertility of uterine origin
    N97.8Female infertility of other origin
    N97.9Female infertility, unspecified
    O09.90Supervision of high risk pregnancy, unspecified, unspecified trimester

    Showing 1–10 of 17

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Carrier Testing Panels for Genetic Diseases (for Kentucky Only)UnitedHealthcare2026-10-01
    Genetic Testing for Neurological Disorders (for Kansas Only)UnitedHealthcare2026-10-01
    Genetic and Molecular TestingProvidence Health Plan2026-10-01
    Cell-Free Fetal DNA Testing (for Idaho Only)UnitedHealthcare2026-10-01
    Next Generation Sequencing for Minimal Residual Disease DetectionProvidence Health Plan2026-10-01
    Pharmacogenetic Panel Testing (for Kansas Only)UnitedHealthcare2026-10-01
    Non-Covered Genetic Panel TestsProvidence Health Plan2026-10-01

    Related Codes

    CPT-89250-SHORT-TERM-OOCYTE-EMBRYO-CULTURE-ART

    CPT 89250: Oocyte or Embryo Culture <4 Days

    CPT-89251-OOCYTE-EMBRYO-SHORT-TERM-COCULTURE-ART

    CPT 89251: Oocyte/Embryo Culture with Coculture (<4 days)

    CPT-89253-ASSISTED-EMBRYO-HATCHING-PARTIAL-ZONA-DISSECTION

    CPT 89253: Assisted Embryo Hatching, Partial Zona Dissection

    CPT-89254-FOLLICULAR-FLUID-OOCYTE-IDENTIFICATION-LAB

    CPT 89254: Oocyte Identification from Follicular Fluid

    CPT-89255-EMBRYO-PREPARATION-FOR-TRANSFER-ART

    CPT 89255: Embryo Preparation for Transfer (ART)

    CPT-89257-SPERM-IDENTIFICATION-FROM-ASPIRATION

    CPT 89257: Identification of Sperm from Non-Seminal Aspirates

    CPT-89259-SPERM-CRYOPRESERVATION-AND-PRESERVATION

    CPT 89259: Sperm Cryopreservation, Laboratory Processing

    CPT-89260-LABORATORY-SPERM-PREPARATION-SPERM-WASH-SWIM-UP

    CPT 89260: Sperm Isolation for Sperm Wash and Swim‑Up Preparation

    CPT-89261-SPERM-PREPARATION-AND-SEMEN-ANALYSIS

    CPT 89261: Complex Sperm Preparation and Semen Analysis

    CPT-89264-SPERM-IDENTIFICATION-TESTICULAR-TISSUE

    CPT 89264: Identification of Sperm from Testicular Tissue

    Showing 1–10 of 36

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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