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    v1.0.30
    OpenPayerBilling CodesReproductive Medicine ProceduresCPT cpt-89260-laboratory-sperm-preparation-sperm-wash-swim-up
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    CPT 89260: Sperm Isolation for Sperm Wash and Swim‑Up Preparation

    CPT code 89260 describes laboratory isolation of sperm using simple preparations such as sperm wash and swim‑up performed during a semen analysis to prepare sperm for insemination or diagnostic evaluation; this is a laboratory reproductive medicine procedure typically performed in an andrology or fertility clinic laboratory or hospital-based clinical laboratory as a specimen processing and sperm preparation service for intrauterine insemination or diagnostic semen assessment.

    For related coverage guidance, see recent payer policy updates: Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency, Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policy.

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    Clinical & Coding Specifications

    Clinical Context

    A 34-year-old couple presents to a fertility clinic after 12 months of unsuccessful conception. The male partner provides a fresh semen specimen on the day of an intrauterine insemination (IUI) procedure. In the andrology laboratory, a trained lab analyst performs a simple sperm preparation technique such as a sperm wash and swim-up to isolate motile sperm for insemination. The workflow includes receipt and accessioning of the specimen, semen analysis (volume, concentration, motility, morphology), performance of the sperm wash and swim-up isolation, post-preparation concentration and motility assessment, and handing the prepared inseminate to the clinical team for immediate use in an IUI or for diagnostic testing. Typical site of service is an outpatient fertility clinic or hospital-based reproductive endocrinology laboratory. The patient scenario also includes cases where the preparation is performed for diagnostic purposes (to assess sperm function) prior to assisted reproductive procedures.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    89260The lab analyst performs the isolation of sperm using a simple preparation such as sperm wash and swim–up during a semen analysis to prepare sperm for insemination or diagnosis.Primary procedure: isolated sperm preparation for IUI or diagnostic assessment
    89050Semen analysis; presence and/or motility, with sperm concentrationPreceding diagnostic step to quantify baseline semen parameters before or after preparation
    89262Sperm preparation for intrauterine insemination, simple preparation; with incubation and layering methodsAlternative or additional preparation technique often performed in the same clinical workflow
    89268Semen preparation for assisted reproductive technology, extended preparation (e.g., Percoll gradient)More complex sperm isolation used for in vitro fertilization or when enhanced separation is required
    89320Heterologous insemination (donor sperm), by intrauterine routeClinical procedure that uses prepared sperm delivered intrauterinely; prepared specimen from 89260 may be used

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207L00000X
    363L00000X
    207RA0201X
    101YP2500X
    225X00000X
    364SP0811X
    2278G1100X
    207XX0005X

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Carrier Testing Panels for Genetic Diseases (for Kentucky Only)UnitedHealthcare2026-10-01
    Genetic Testing for Neurological Disorders (for Kansas Only)UnitedHealthcare2026-10-01
    Genetic and Molecular TestingProvidence Health Plan2026-10-01
    Cell-Free Fetal DNA Testing (for Idaho Only)UnitedHealthcare2026-10-01
    Next Generation Sequencing for Minimal Residual Disease DetectionProvidence Health Plan2026-10-01
    Pharmacogenetic Panel Testing (for Kansas Only)UnitedHealthcare2026-10-01
    Non-Covered Genetic Panel TestsProvidence Health Plan2026-10-01

    Related Codes

    CPT-89250-SHORT-TERM-OOCYTE-EMBRYO-CULTURE-ART

    CPT 89250: Oocyte or Embryo Culture <4 Days

    CPT-89251-OOCYTE-EMBRYO-SHORT-TERM-COCULTURE-ART

    CPT 89251: Oocyte/Embryo Culture with Coculture (<4 days)

    CPT-89253-ASSISTED-EMBRYO-HATCHING-PARTIAL-ZONA-DISSECTION

    CPT 89253: Assisted Embryo Hatching, Partial Zona Dissection

    CPT-89254-FOLLICULAR-FLUID-OOCYTE-IDENTIFICATION-LAB

    CPT 89254: Oocyte Identification from Follicular Fluid

    CPT-89255-EMBRYO-PREPARATION-FOR-TRANSFER-ART

    CPT 89255: Embryo Preparation for Transfer (ART)

    CPT-89257-SPERM-IDENTIFICATION-FROM-ASPIRATION

    CPT 89257: Identification of Sperm from Non-Seminal Aspirates

    CPT-89258-EMBRYO-CRYOPRESERVATION-IVF

    CPT 89258: Embryo Cryopreservation During IVF Cycle

    CPT-89259-SPERM-CRYOPRESERVATION-AND-PRESERVATION

    CPT 89259: Sperm Cryopreservation, Laboratory Processing

    CPT-89261-SPERM-PREPARATION-AND-SEMEN-ANALYSIS

    CPT 89261: Complex Sperm Preparation and Semen Analysis

    CPT-89264-SPERM-IDENTIFICATION-TESTICULAR-TISSUE

    CPT 89264: Identification of Sperm from Testicular Tissue

    Showing 1–10 of 36

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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