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    v1.0.28
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86834-hla-class-i-antibody-solid-phase-immunoassay
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    CPT 86834: HLA Class I Antibody Solid Phase Immunoassay

    CPT code 86834 describes a laboratory solid phase immunoassay performed on patient serum to quantify specific antibodies to Class I human leukocyte antigens (HLA); the service is a serologic antibody testing procedure typically performed in a clinical immunology or transplant laboratory as an outpatient diagnostic laboratory service to assess HLA sensitization prior to transplantation or for immunologic evaluation.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immune Cell Function Assay, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 45-year-old female kidney transplant recipient presents for routine post-transplant immunologic monitoring and for evaluation of declining graft function. Her transplant nephrologist orders donor-specific antibody testing to quantify recipient serum antibodies directed against Class I human leukocyte antigens (HLA-A, HLA-B, HLA-C) to assess for antibody-mediated rejection risk. In the clinical workflow: the phlebotomy team collects a serum sample, labels it with patient and donor identifiers, and sends it to the hospital immunology laboratory. A laboratory analyst performs a solid-phase immunoassay to quantify specific anti–HLA Class I antibodies. The lab documents assay results, including mean fluorescence intensity or equivalent quantitative values, interprets positive/negative thresholds, and reports results to the ordering clinician. Results inform decisions about immunosuppression adjustment, further confirmatory testing, or protocol biopsies as clinically appropriate.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86834Solid phase immunoassay for HLA Class I antibodies, serum — Quantitative assay performed by lab analystPrimary code describing the performed assay to quantify specific Class I HLA antibodies in patient serum
    86830T and B lymphocyte crossmatch (complement-dependent cytotoxicity)Often performed alongside HLA antibody testing as a historical or complementary compatibility test prior to transplantation
    86900Blood compatibility tests; modified (e.g., enzyme treated)May be part of pre-transplant immunologic workup in conjunction with HLA antibody assays
    86701Antigen; respiratory virus serology (not directly related)Data not available in the input
    88291Interpretation and reporting of cytogenetic microarray testing; complex (not directly related)Data not available in the input
    86356Cellular antigen identification by flow cytometryMay be used adjunctively for assessing lymphocyte subsets or flow-based crossmatch in transplant workup

    Related Modifiers

    7 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207P00000XEmergency Medicine PhysicianAn emergency physician focuses on the immediate decision making and action necessary to prevent death or any further disability both in the pre-hospit…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    363A00000XPhysician AssistantA physician assistant is a person who has successfully completed an accredited education program for physician assistant, is licensed by the state and…
    251T00000XPACE Provider OrganizationA PACE provider organization is a not-for-profit private or public entity that is primarily engaged in providing PACE services(unique capitated manage…
    207SM0001XMolecular Genetic Pathology (Medical Genetics) PhysicianA board certified subspecialty, the molecular genetic pathologist is expert in the principles, theory and technologies of molecular biology and molecu…
    2086S0122XPlastic and Reconstructive Surgery PhysicianA surgeon who specializes in plastic and reconstructive surgery.
    103TF0000XFamily PsychologistA psychologist whose specialty is founded on principles of systems theory with the interpersonal system of the family the focus of assessment, interve…
    363LC1500XCommunity Health Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner

    Related ICD-10-CM Codes

    25 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    D47.1Chronic myeloproliferative disease
    D47.Z2Castleman disease
    D63.1Anemia in chronic kidney disease
    D72.829Elevated white blood cell count, unspecified
    E88.01Alpha-1-antitrypsin deficiency
    I12.9Hypertensive chronic kidney disease with stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
    I13.0Hypertensive heart and chronic kidney disease with heart failure and stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
    I50.9Heart failure, unspecified
    N18.4Chronic kidney disease, stage 4 (severe)

    Showing 1–10 of 25

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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