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    v1.0.30
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86825-hla-crossmatch-flow-cytometry
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    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    CPT code 86825 describes an HLA crossmatch assay in which a laboratory analyst tests donor lymphocytes against an initial recipient serum sample or dilution using non-cytotoxic methods (for example, flow cytometry) to assess human leukocyte antigen compatibility; this is a specialized immunogenetics service typically performed in a clinical immunology or histocompatibility laboratory and reported as a laboratory-based crossmatch procedure.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies, Immune Cell Function Assay.

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    Clinical & Coding Specifications

    Clinical Context

    A typical patient scenario involves a patient being evaluated for hematopoietic stem cell transplantation or a solid-organ transplant where HLA compatibility between donor lymphocytes and recipient serum is critical. A transplant coordinator orders an HLA crossmatch using flow cytometry after initial HLA typing to assess the presence of recipient anti-donor antibodies. The lab receives a donor lymphocyte sample and the recipient’s serum; a histocompatibility laboratory technologist prepares cell suspensions, incubates recipient serum with donor lymphocytes, and runs a flow cytometry–based crossmatch. Results document either a positive or negative crossmatch and antibody strength, which are reported back to the transplant team to guide donor selection and perioperative immunologic management. Typical sites of service include hospital-based histocompatibility laboratories, independent reference immunology laboratories, and transplant center laboratories. The patient is typically an adult or pediatric candidate for bone marrow, stem cell, or organ transplantation, often with ICD-10 diagnoses related to end-stage organ disease or hematologic malignancy leading to the need for transplant evaluation.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86900Crossmatch; major and minor blood groups (initial crossmatch)Blood bank crossmatching for red cell compatibility often performed as part of pre-transplant infectious/immunologic workup; complementary to HLA crossmatch for transfusion planning.
    86816HLA typing, low- to intermediate-resolutionHLA typing is performed before crossmatch to identify HLA antigens; this precedes or accompanies 86825 in donor-recipient compatibility evaluation.
    86850HLA typing, high-resolution sequence-based typingHigh-resolution HLA typing refines allele-level matches used in transplant matching and informs interpretation of crossmatch results.
    86830HLA antibody identification, any method (single antigen, Luminex)Antibody identification and specificity testing are frequently performed alongside or prior to flow cytometry crossmatch to define anti-HLA antibodies.
    86353Antibody; screen, qualitative (e.g., antinuclear antibody) (Note: general immunology test)Pre-transplant immunologic screening tests may be ordered in the same workflow; used when broader antibody screening is required.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RH0000X
    2084P0800X
    211D00000X
    2080P0008X
    251B00000X
    1835P1300X
    251F00000X
    207P00000X

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86826-HLA-CROSSMATCH-FLOW-CYTOMETRY-BASED-COMPATIBILITY-TEST

    CPT 86826: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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