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    v1.0.30
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86816-hla-class-ii-serologic-single-antigen-typing-dr-dq
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    CPT 86816: HLA Class II Single Antigen Serology

    CPT code 86816 describes a serologic laboratory test performed by a lab analyst to identify a single human leukocyte antigen (HLA) of the Class II types DR or DQ; this is a HLA Class II single antigen serology procedure typically performed as a specialized laboratory immunogenetics service on blood or cellular specimens in a clinical laboratory or reference lab setting.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies, Immune Cell Function Assay.

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old patient with end-stage renal disease is being evaluated for kidney transplantation. The transplant center orders HLA Class II typing focused on DR and DQ loci to identify recipient HLA antigens for donor-recipient matching and crossmatch interpretation. A phlebotomy technician obtains a blood sample in an anticoagulated tube and sends it to the immunogenetics laboratory. The laboratory analyst performs a serologic HLA Class II test for one or more DR and/or DQ specificities using standard microlymphocytotoxicity or flow-based serologic methods; results are reported to the transplant team and entered into the laboratory information system. The workflow includes specimen accessioning, cell separation or preparation, performance of the serologic assay, quality control, interpretation by a qualified laboratory professional, and issuance of a final report that feeds into donor selection and anti-HLA antibody assessment.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86816HLA serologic typing for one HLA Class II antigen (DR or DQ)Primary procedure: identification of a single HLA Class II antigen (DR or DQ) by serologic methods.
    86810HLA serologic typing, class I (A, B)Performed alongside class II typing to provide a full HLA profile for transplant matching when class I antigens are required.
    86830HLA molecular typing, each locus (example: DRB1)Molecular methods (PCR-based) often performed in addition to or as confirmatory testing to serologic class II typing.
    86900Antibody screening; platelet or red cell antibody screen (single or multiple antigen)Performed to assess recipient alloantibodies that may affect transplant compatibility and crossmatch interpretation.
    86860Crossmatch, lymphocyte (direct or indirect)Direct crossmatch testing between donor lymphocytes and recipient serum is used after HLA typing to determine immunologic compatibility.
    86353Antibody detection by indirect immunofluorescence or other techniqueUsed for detection of anti-HLA antibodies complementing serologic typing results.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    23Modifier -23 (Unusual Anesthesia) is used on anesthesia service CPT codes when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia…- Use modifier -23 only on anesthesia CPT codes (00100–01999) when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia because of…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RH0000X
    207L00000X
    302R00000X
    2279G0305X
    2472E0500X
    364SP0807X
    2279H0200X
    207KA0200X

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    CPT-86826-HLA-CROSSMATCH-FLOW-CYTOMETRY-BASED-COMPATIBILITY-TEST

    CPT 86826: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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