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    v1.0.28
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86828-hla-antibody-solid-phase-immunoassay
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    CPT 86828: HLA Antibody Solid Phase Immunoassay

    CPT code 86828 describes a laboratory solid phase immunoassay performed on patient serum to detect the presence or absence of human leukocyte antigen (HLA) Class I and Class II antibodies, a diagnostic immunology test used in transplant compatibility and alloimmune assessment; the service type is clinical laboratory testing and the typical site of service is a hospital or independent clinical laboratory or transplant reference laboratory performing serologic HLA antibody screening and identification.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immune Cell Function Assay, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 46-year-old woman with end-stage renal disease is being evaluated for kidney transplantation. The transplant center orders HLA antibody testing to assess donor-specific antibodies and sensitization status prior to listing and before a planned transplant. A phlebotomy technician collects a serum specimen and sends it to the hospital reference immunology laboratory. A clinical laboratory scientist performs a solid-phase immunoassay using patient serum to detect antibodies to HLA Class I and II antigens (CPT 86828). Results are reported in the electronic medical record and used by the transplant immunology team to determine crossmatch risk, guide donor selection, and inform desensitization strategies. Typical site of service is an outpatient laboratory or hospital clinical immunology lab; specimens may originate from outpatient transplant clinics, dialysis units, or inpatient wards. Turnaround time is usually 24–72 hours depending on workflow and urgency.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86828Antibody identification by solid phase (HLA) — Class I and IIPrimary assay to detect HLA class I and II antibodies; used for donor-specific antibody assessment.
    86829Crossmatch testing, flow cytometry or complement-dependent cytotoxicityPerformed to evaluate compatibility between donor cells and recipient serum; often follows HLA antibody detection.
    86900Blood compatibility tests (e.g., crossmatch)Serologic crossmatch procedures that may be used alongside HLA antibody testing during pre-transplant evaluation.
    83020Hemoglobin A1cExample of routine lab tests often ordered in transplant workup; not directly related to HLA but common in pre-transplant panels.
    86350Antibody identification (non-HLA), serologyOther antibody identification assays that may be ordered in parallel when immunologic history is complex.

    Note: If an institution bills separated components, TC or 26 modifiers may be appended to the technical or professional components of lab services as applicable.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    2084P0800XPsychiatry PhysicianA Psychiatrist specializes in the prevention, diagnosis, and treatment of mental disorders, emotional disorders, psychotic disorders, mood disorders, …
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    207ZP0101XAnatomic Pathology PhysicianA pathologist deals with the causes and nature of disease and contributes to diagnosis, prognosis and treatment through knowledge gained by the labora…
    3336N0007XNuclear PharmacyA pharmacy dedicated to the compounding and dispensing of radioactive materials for use in nuclear imaging and nuclear medical procedures.
    2081P0004XSpinal Cord Injury Medicine PhysicianA physician who addresses the prevention, diagnosis, treatment and management of traumatic spinal cord injury and non-traumatic etiologies of spinal c…
    261QV0200XVA Clinic/CenterAmbulatory Health Care Facilities · Clinic/Center
    163WI0600XInfection Control Registered NurseNursing Service Providers · Registered Nurse

    Related ICD-10-CM Codes

    26 rows

    ICD-10-CM CodeDescription
    D47.Z2Castleman disease
    I12.9Hypertensive chronic kidney disease with stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
    I50.9Heart failure, unspecified
    L93.0Discoid lupus erythematosus
    M05.79Rheumatoid arthritis with rheumatoid factor of multiple sites without organ or systems involvement
    M06.9Rheumatoid arthritis, unspecified
    M32.9Systemic lupus erythematosus, unspecified
    N18.5Chronic kidney disease, stage 5
    N18.6End stage renal disease
    T86.10Unspecified complication of kidney transplant

    Showing 1–10 of 26

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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