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    v1.0.28
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86831-hla-class-ii-antibody-solid-phase-immunoassay
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    CPT 86831: HLA Class II Antibody Solid Phase Immunoassay

    CPT code 86831 describes a solid phase immunoassay performed on patient serum to detect antibodies to HLA Class II antigens using a panel of complete Class II phenotypes; the service is a laboratory serologic test generally provided in a clinical laboratory or hospital laboratory setting and is used in immunology and transplant compatibility evaluation.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immune Cell Function Assay, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old woman with end-stage renal disease is being evaluated for kidney transplantation. The transplant team orders HLA Class II antibody testing to detect recipient antibodies against donor HLA-DR, -DQ, and -DP antigens. A phlebotomy specimen of patient serum is collected in the outpatient transplant clinic and sent to the immunology laboratory. A lab analyst performs a solid-phase immunoassay (CPT 86831) using a panel of complete HLA Class II phenotypes to identify specific anti-HLA Class II antibodies. Results are reported to the transplant coordinator and transplant nephrologist to inform donor selection and crossmatch planning. The typical workflow includes specimen receipt and accessioning, serum separation, testing on the solid-phase platform, data review by a qualified technologist, and electronic reporting to the electronic health record and transplant database. Typical sites of service are hospital clinical laboratories, freestanding reference immunology laboratories, and transplant center laboratories.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86831Solid phase immunoassay for HLA Class II antibodies using patient serumPrimary procedure described; identifies specific anti-HLA Class II antibodies for transplant compatibility.
    86816HLA Class I antibody identification, solid phasePerformed alongside Class II testing to assess antibodies against HLA Class I antigens (complements Class II results).
    86815HLA antibody screen, solid phase (single or multiple system)Often used as an initial screen prior to detailed Class I/II identification assays.
    86830Solid phase immunoassay for HLA Class I antibodies using patient serum (Note: similar to 86816)Used in parallel workflows for complete HLA antibody profiling.
    88360Immunohistochemistry, per specimen; initial single antibody stainMay be used in donor tissue typing workflows when tissue HLA expression needs confirmation.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207RG0100XGastroenterology PhysicianAn internist who specializes in diagnosis and treatment of diseases of the digestive organs including the stomach, bowels, liver and gallbladder. This…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    2278H0200XHome Health Certified Respiratory TherapistHome care fosters individual responsibility for self-management of chronic respiratory conditions. It includes individualized assessment based plans …
    207YS0012XSleep Medicine (Otolaryngology) PhysicianAn Otolaryngologist who practices Sleep Medicine is certified in the subspecialty of sleep medicine and specializes in the clinical assessment, physio…
    2083X0100XOccupational Medicine PhysicianOccupational medicine focuses on the health of workers, including the ability to perform work; the physical, chemical, biological, and social environm…
    208VP0000XPain Medicine PhysicianPain Medicine is a primary medical specialty based on a distinct body of knowledge and a well-defined scope of clinical practice that is founded on sc…
    341600000XAmbulanceAn emergency vehicle used for transporting patients to a health care facility after injury or illness. Types of ambulances used in the United States i…

    Related ICD-10-CM Codes

    27 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    C34.10Malignant neoplasm of upper lobe, unspecified bronchus or lung
    C34.90Malignant neoplasm of unspecified part of unspecified bronchus or lung
    C34.91Malignant neoplasm of unspecified part of right bronchus or lung
    C43.9Malignant melanoma of skin, unspecified
    D47.Z9Other specified neoplasms of uncertain behavior of lymphoid, hematopoietic and related tissue
    D86.9Sarcoidosis, unspecified
    D89.82Autoimmune lymphoproliferative syndrome [ALPS]
    I12.9Hypertensive chronic kidney disease with stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
    I50.9Heart failure, unspecified

    Showing 1–10 of 27

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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