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    v1.0.30
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86812-hla-class-i-single-antigen-serologic-test
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    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT code 86812 describes a serologic laboratory test for identification of a single human leukocyte antigen (HLA) Class I antigen (A, B, or C), performed by a laboratory analyst; service type is HLA serologic typing and the typical site of service is a clinical laboratory or hospital lab performing histocompatibility testing.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies, Immune Cell Function Assay.

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old patient undergoing evaluation for kidney transplantation is referred to the histocompatibility laboratory for HLA Class I antigen typing. The clinician orders a single-antigen serologic assay to determine whether the patient expresses a specific HLA-A, HLA-B, or HLA-C antigen that may affect donor compatibility or risk of sensitization. The typical workflow: the transplant coordinator obtains a blood sample and sends it to the hospital or reference immunogenetics laboratory; a medical technologist or lab analyst performs the serologic test using reagent antisera and microscopy or automated platforms; results are reviewed by a pathologist or HLA laboratory director and reported to the transplant team for donor selection and crossmatch planning. This service is commonly performed in an outpatient collection setting, hospital laboratory, or specialized reference immunogenetics laboratory and supports pre-transplant workup, organ allocation decisions, and transfusion or immunogenetics consultations.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86816HLA class II typing by serologic methodsPerformed when additional HLA Class II antigen information is needed for donor-recipient matching.
    86813HLA class I typing, each additional antigen identified (when applicable)Billed when multiple class I antigens are identified in addition to the primary antigen coded with 86812.
    86825HLA crossmatch, lymphocyte (e.g., AHG)Performed after HLA typing to assess recipient antibodies against donor lymphocytes for compatibility.
    86850HLA antibody identification by solid phase or other methodsUsed to identify specific anti-HLA antibodies complementing serologic antigen typing in sensitized patients.
    86900Autoantibody, each procedure (screening methods)May be performed in parallel when broader immunologic screening is indicated in transplant evaluation.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RH0000X
    207L00000X
    364SG0600X
    207RI0001X
    2251H1200X
    3747A0650X
    163WW0000X
    171R00000X

    Related ICD-10-CM Codes

    29 rows

    ICD-10-CM CodeDescription
    A34Obstetrical tetanus
    B20Human immunodeficiency virus [HIV] disease
    D47.Z9Other specified neoplasms of uncertain behavior of lymphoid, hematopoietic and related tissue
    D56.9Thalassemia, unspecified
    D57.1Sickle-cell disease without crisis
    D66Hereditary factor VIII deficiency
    D67Hereditary factor IX deficiency
    D68.9Coagulation defect, unspecified
    D69.3Immune thrombocytopenic purpura
    O35.4XX0Maternal care for (suspected) damage to fetus from alcohol, not applicable or unspecified

    Showing 1–10 of 29

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    CPT-86826-HLA-CROSSMATCH-FLOW-CYTOMETRY-BASED-COMPATIBILITY-TEST

    CPT 86826: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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