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    v1.0.28
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86830-hla-class-i-antibody-solid-phase-immunoassay
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    CPT 86830: HLA Class I Antibody Solid-Phase Immunoassay

    CPT code 86830 describes a laboratory solid-phase immunoassay performed on patient serum to detect antibodies to specific HLA Class I antigens from a panel of complete Class I phenotypes; the service is a histocompatibility (HLA) antibody testing procedure typically performed in a clinical immunology or histocompatibility laboratory setting, often as part of pre-transplant antibody screening or post-transplant monitoring.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immune Cell Function Assay, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies.

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    Clinical & Coding Specifications

    Clinical Context

    A 45-year-old patient with end-stage renal disease is evaluated for kidney transplantation. The transplant program orders antibody screening for donor-specific HLA Class I antibodies prior to listing and again during donor workup. A phlebotomy technician collects a serum sample which is sent to the laboratory. A medical laboratory scientist performs a solid-phase immunoassay using patient serum to detect antibodies to HLA Class I antigens from a complete panel of Class I phenotypes, documenting antibody specificity and strength. Results are reported to the transplant physician and transplant immunology team to guide donor selection, crossmatching decisions, and immunosuppression planning. Typical site of service is an outpatient clinical laboratory or hospital-based immunology lab performing specialized transplant testing.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86830The lab analyst performs a solid phase immunoassay using patient serum to identify the presence of specific antibodies to specific Class I human leukocyte antigens, or HLA, from a panel of complete HLA Class I phenotypes.Primary assay for detecting HLA Class I antibodies in transplant evaluation
    86850The lab analyst performs a solid phase immunoassay using patient serum to identify the presence of specific antibodies to HLA Class II antigens from a panel of complete HLA Class II phenotypes.Often performed alongside 86830 to assess Class II HLA antibodies for comprehensive donor compatibility assessment
    86816Crossmatch by anti-human globulin (AHG) enhanced complement-dependent cytotoxicity (CDC) technique.Performed in some transplant programs to assess donor-specific reactivity; complements solid-phase antibody testing
    86860HLA specific antibody identification, single antigen bead testing, per antigen.Used when more granular single antigen specificity and strength information is required following screening assays like 86830
    86465Antibody; HIV-1 and HIV-2, confirmatory by Western blot, or other confirmatory testing.Example of laboratory confirmatory testing workflow; not specific to HLA but illustrates specialized serologic testing performed in reference labs

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207R00000XInternal Medicine PhysicianA physician who provides long-term, comprehensive care in the office and the hospital, managing both common and complex illness of adolescents, adults…
    208000000XPediatrics PhysicianA pediatrician is concerned with the physical, emotional and social health of children from birth to young adulthood. Care encompasses a broad spectru…
    2084P0800XPsychiatry PhysicianA Psychiatrist specializes in the prevention, diagnosis, and treatment of mental disorders, emotional disorders, psychotic disorders, mood disorders, …
    363LF0000XFamily Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner
    156FX1700XOcularistAn ocularist is a thoroughly trained professional skilled in the art of fitting, painting, and fabricating custom ocular prostheses. In addition to cr…
    2080P0205XPediatric Endocrinology PhysicianA pediatrician who provides expert care to infants, children and adolescents who have diseases that result from an abnormality in the endocrine glands…
    207P00000XEmergency Medicine PhysicianAn emergency physician focuses on the immediate decision making and action necessary to prevent death or any further disability both in the pre-hospit…
    208C00000XColon & Rectal Surgery PhysicianA colon and rectal surgeon is trained to diagnose and treat various diseases of the intestinal tract, colon, rectum, anal canal and perianal area by m…

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    D56.9Thalassemia, unspecified
    D63.8Anemia in other chronic diseases classified elsewhere
    D86.0Sarcoidosis of lung
    D89.9Disorder involving the immune mechanism, unspecified
    E83.52Hypercalcemia
    I12.9Hypertensive chronic kidney disease with stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
    I21.9Acute myocardial infarction, unspecified
    I50.9Heart failure, unspecified
    K76.9Liver disease, unspecified

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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