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    v1.0.28
    OpenPayerBilling CodesTissue Typing Immunological ProceduresCPT cpt-86832-hla-class-i-antibody-solid-phase-immunoassay
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    CPT 86832: HLA Class I Single Antigen Solid Phase Immunoassay

    CPT code 86832 describes a laboratory solid phase immunoassay performed on patient serum to detect antibodies to specific Class I human leukocyte antigens (HLA) using a high‑definition method such as testing one antigen per bead; the service is a specialized immunogenetics antibody detection assay typically performed in a clinical immunology or histocompatibility laboratory (laboratory service) and is commonly done in outpatient or reference lab settings.

    For related coverage guidance, see recent payer policy updates: Biomarker Testing for Autoimmune Rheumatic Disease, Immune Cell Function Assay, Immunopharmacologic Monitoring of Therapeutic Serum Antibodies.

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    Clinical & Coding Specifications

    Clinical Context

    A 45-year-old woman with end-stage renal disease is evaluated prior to kidney transplantation. The transplant team orders testing to detect recipient anti–HLA Class I antibodies against potential donor antigens. Blood is drawn in the outpatient phlebotomy area and serum is sent to the immunology laboratory. A lab analyst performs a solid-phase, single-antigen bead immunoassay (86832) using patient serum to identify antibodies to specific Class I human leukocyte antigens (HLA-A, HLA-B, HLA-C). Results are reviewed by the histocompatibility specialist and incorporated into the crossmatch and donor selection workflow. The typical site of service is an outpatient hospital laboratory or freestanding reference immunology lab that supports transplant services. Turnaround time is usually 24–72 hours depending on batching and confirmatory testing.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86832Solid phase immunoassay for HLA Class I antibodies, single antigen bead, high-definition methodPrimary assay to detect specific anti–HLA Class I antibodies in recipient serum.
    86816Complement-dependent cytotoxicity crossmatchFunctional crossmatch performed to assess donor–recipient compatibility; may follow antibody identification.
    86840Flow cytometry crossmatchAlternative crossmatch technique used by transplant programs to confirm donor-specific antibodies detected by single-antigen assays.
    86255Solid phase assay for HLA antibodies (screening)Initial antibody screen that may precede single-antigen testing to define broad sensitization.
    86430Tissue typing; HLA antibody identification, non-specific (panel)Broader panel-based HLA antibody identification method that complements single-antigen high-definition testing.

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207Q00000XFamily Medicine PhysicianFamily Medicine is the medical specialty which is concerned with the total health care of the individual and the family. It is the specialty in breadt…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    152WV0400XVision Therapy OptometristOptometrists who specialize in vision therapy as a treatment process used to improve vision function. It includes a broad range of developmental and r…
    2084P2900XPain Medicine (Psychiatry & Neurology) PhysicianA neurologist, child neurologists or psychiatrist who provides a high level of care, either as a primary physician or consultant, for patients experie…
    2255R0406XBlind Rehabilitation Specialist/TechnologistRespiratory, Developmental, Rehabilitative and Restorative Service Providers · Specialist/Technologist
    246YR1600XRegistered Record AdministratorNone
    207RA0001XAdvanced Heart Failure and Transplant Cardiology PhysicianSpecialists in Advanced Heart Failure and Transplant Cardiology would participate in the inpatient and outpatient management of patients with advanced…
    207RX0202XMedical Oncology PhysicianAn internist who specializes in the diagnosis and treatment of all types of cancer and other benign and malignant tumors. This specialist decides on a…

    Related ICD-10-CM Codes

    27 rows

    ICD-10-CM CodeDescription
    D47.Z2Castleman disease
    D80.0Hereditary hypogammaglobulinemia
    D80.1Nonfamilial hypogammaglobulinemia
    D80.2Selective deficiency of immunoglobulin A [IgA]
    D84.9Immunodeficiency, unspecified
    I21.9Acute myocardial infarction, unspecified
    I50.9Heart failure, unspecified
    I63.9Cerebral infarction, unspecified
    L93.0Discoid lupus erythematosus
    M32.9Systemic lupus erythematosus, unspecified

    Showing 1–10 of 27

    Related Payer Policy Updates

    Specialty: Immunology/Rheumatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Biomarker Testing for Autoimmune Rheumatic DiseaseOscar Health2026-10-01
    Immune Cell Function AssayOscar Health2026-10-01
    Immunopharmacologic Monitoring of Therapeutic Serum AntibodiesOscar Health2026-10-01
    Biomarker Testing for Autoimmune Rheumatic DiseaseBlue Cross Blue Shield - New Mexico2026-09-04
    Inflammatory Conditions - Orencia Intravenous Preferred Specialty Management Policy for Legacy Prescription Drug ListsCigna2026-09-01
    Secukinumab (Cosentyx) — intravenous (Medicare Advantage)blue cross blue shield - louisiana2026-09-01
    Intravenous Immune Globulin (IVIG) — indications and authorization criteriaBlue Cross Blue Shield - Iowa2026-08-28
    Infliximab Intravenous Products Preferred Specialty Management PolicyCigna2026-08-15
    Kineret (anakinra) — Coverage CriteriaBlue Cross Blue Shield - Iowa2026-08-14
    Actimmune (interferon gamma-1b) — Prior Authorization and Coverage Criteriameridian_health_plan_of_michigan2026-08-01

    Related Codes

    CPT-86805-TRANSPLANT-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86805: Lymphocyte Crossmatch with Recipient Serum

    CPT-86806-SEROLOGIC-CROSSMATCH-DONOR-LYMPHOCYTE-RECIPIENT-SERUM

    CPT 86806: Lymphocyte Crossmatch, Donor Lymphocytes with Recipient Serum

    CPT-86807-HLA-ANTIBODY-SCREENING-TRANSPLANT-COMPATIBILITY

    CPT 86807: HLA Antibody Percent Reactive Screening

    CPT-86808-HLA-PERCENT-REACTIVE-ANTIBODY-SCREENING

    CPT 86808: HLA Percent Reactive Antibody Screen

    CPT-86812-HLA-CLASS-I-SINGLE-ANTIGEN-SEROLOGIC-TEST

    CPT 86812: HLA Class I Antigen Serologic Typing

    CPT-86813-HLA-CLASS-I-SEROLOGIC-TYPING

    CPT 86813: HLA Class I Serologic Typing (A, B, C)

    CPT-86816-HLA-CLASS-II-SEROLOGIC-SINGLE-ANTIGEN-TYPING-DR-DQ

    CPT 86816: HLA Class II Single Antigen Serology

    CPT-86817-HLA-CLASS-II-DR-DQ-SEROLOGIC-IDENTIFICATION

    CPT 86817: HLA Class II DR/DQ Serologic Typing

    CPT-86821-HLA-LYMPHOCYTE-CROSSMATCH-COMPATIBILITY-TEST

    CPT 86821: Lymphocyte Crossmatch for HLA Incompatibility

    CPT-86825-HLA-CROSSMATCH-FLOW-CYTOMETRY

    CPT 86825: HLA Crossmatch by Non-Cytotoxic Method

    Showing 1–10 of 19

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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