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    v1.0.30
    OpenPayerBilling CodesDefinitive Drug Testing ProceduresCPT cpt-80335-tricyclic-antidepressant-assay
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    CPT 80335: Tricyclic and Cyclical Antidepressant Assay

    CPT code 80335 describes a laboratory assay to measure the amount of or detect the presence of one or two tricyclic and other cyclical antidepressants in a patient specimen, a toxicology/therapeutic drug monitoring test typically performed by clinical laboratories using blood or urine samples and billed as a laboratory diagnostic service; the service type is drug assay/toxicology testing and the typical site of service is a clinical laboratory or hospital lab.

    For related coverage guidance, see recent payer policy updates: Hematology - Adzynma, Tumor Treating Fields for Pancreatic Cancer, Axicabtagene ciloleucel (Yescarta®) coverage.

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old patient treated for major depressive disorder presents to an outpatient laboratory after their psychiatrist requests drug monitoring to assess adherence and therapeutic drug levels of tricyclic and other cyclical antidepressants. A blood specimen is collected in the ambulatory phlebotomy area. The laboratory receives the specimen, performs an immunoassay or chromatographic testing per protocol to detect and quantify one or two tricyclic and related cyclical antidepressants, documents results in the electronic medical record, and reports findings to the ordering clinician for medication management and safety monitoring. Typical sites of service include outpatient hospital laboratories, independent clinical laboratories, and hospital inpatient labs when monitoring is ordered during admission. The clinical workflow includes order entry, specimen collection, specimen accessioning, analytical testing, quality control, result verification by a laboratory professional, and transmission of results to the treating psychiatrist or primary care provider.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80335Toxicology testing, tricyclic and other cyclical antidepressants; qualitative or quantitative, single or multiple analytes for one or two drugsPrimary assay to detect/measure tricyclic and cyclical antidepressants in patient specimens.
    80307Drug testing, presumptive, any number of drug classes; any number of devices or procedures, single or multiplexMay be used as initial broad-screen toxicology before specific antidepressant confirmation.
    80306Drug testing, presumptive, any number of drug classes; multiple drug class screenUsed for initial point-of-care or lab-based screening for many drug classes including antidepressants.
    80320Drug confirmation by chromatography, qualitative or quantitative, each specimenUsed when confirmation of antidepressant presence/levels is required after screening; often LC-MS/MS or GC-MS.
    80318Drug testing, confirmatory; each specimenAlternative confirmatory code for quantitation/confirmation methods following presumptive testing.

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    208D00000X
    208000000X
    2084P0800X
    2084S0012X
    3418M1110X
    207ZC0500X
    2084P0005X
    207QB0002X

    Related ICD-10-CM Codes

    26 rows

    ICD-10-CM CodeDescription
    F06.31Mood disorder due to known physiological condition with depressive features
    F12.20Cannabis dependence, uncomplicated
    F19.20Other psychoactive substance dependence, uncomplicated
    F31.4Bipolar disorder, current episode depressed, severe, without psychotic features
    F31.5Bipolar disorder, current episode depressed, severe, with psychotic features
    F32.0Major depressive disorder, single episode, mild
    F32.1Major depressive disorder, single episode, moderate
    F32.2Major depressive disorder, single episode, severe without psychotic features
    F32.9Major depressive disorder, single episode, unspecified
    F33.1Major depressive disorder, recurrent, moderate

    Showing 1–10 of 26

    Related Payer Policy Updates

    Specialty: Hematology/Oncology1 / 2

    10 rows

    PolicyPayerEffective Date
    Hematology - AdzynmaCigna2026-10-15
    Tumor Treating Fields for Pancreatic Cancerselect_health2026-10-01
    Axicabtagene ciloleucel (Yescarta®) coverageBaylor Scott & White Health Plan2026-10-01
    Electric Tumor Treatment Field Therapy (for Kentucky Only)UnitedHealthcare2026-10-01
    Electric Tumor Treatment Field Therapy (for Kansas Only)UnitedHealthcare2026-10-01
    Electric Tumor Treatment Field Therapy (for Idaho Only)UnitedHealthcare2026-10-01
    Brexucabtagene autoleucel (Tecartus) CoverageBaylor Scott & White Health Plan2026-10-01
    Electric Tumor Treatment Field Therapy (for Pennsylvania Only)UnitedHealthcare2026-10-01
    Epithelial Cell Cytology in Breast Cancer Risk AssessmentOscar Health2026-10-01
    Factor VIII ProductsCigna2026-09-15

    Related Codes

    CPT-80320-ALCOHOL-DETECTION-IN-SPECIMEN

    CPT 80320: Alcohol Detection and Quantification

    CPT-80321-ALCOHOL-BIOMARKER-ASSAY

    CPT 80321: Alcohol Biomarker Assay, One or Two Compounds

    CPT-80322-ALCOHOL-BIOMARKER-PANEL-MULTI-ANALYTE

    CPT 80322: Alcohol Biomarker Panel, Three or More Compounds

    CPT-80323-ALKALOID-DRUG-LEVEL-ANALYSIS

    CPT 80323: Alkaloid Drug Identification and Quantitation

    CPT-80324-AMPHETAMINE-DRUG-TESTING-LAB-ASSAY

    CPT 80324: Amphetamine Detection and Quantification

    CPT-80325-AMPHETAMINES-DETECTION-AND-QUANTIFICATION

    CPT 80325: Detection of Three or Four Amphetamines

    CPT-80326-AMPHETAMINES-PANEL-5-OR-MORE

    CPT 80326: Detection/Quantification of Five or More Amphetamines

    CPT-80334-SEROTONERGIC-ANTIDEPRESSANT-PANEL

    CPT 80334: Detection of Six or More Serotonergic Antidepressants

    CPT-80344-MULTI-ANTIPSYCHOTIC-PANEL-SEVEN-PLUS-DRUGS

    CPT 80344: Multiplex Antipsychotic Drug Detection (7+ agents)

    CPT-80345-BARBITURATE-DETECTION-SERUM-URINE

    CPT 80345: Barbiturate Detection in Serum or Urine

    Showing 1–10 of 24

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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