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    v1.0.30
    OpenPayerBilling CodesDefinitive Drug Testing ProceduresCPT cpt-80321-alcohol-biomarker-assay
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    CPT 80321: Alcohol Biomarker Assay, One or Two Compounds

    CPT code 80321 describes a laboratory assay in which a clinical laboratory analyst detects and quantifies one or two alcohol biomarker compounds in a patient specimen to indicate recent alcohol exposure; this is a clinical laboratory test typically performed in a clinical laboratory or hospital laboratory setting using blood or other biological specimens.

    For related coverage guidance, see recent payer policy updates: Hematology - Adzynma, Tumor Treating Fields for Pancreatic Cancer, Axicabtagene ciloleucel (Yescarta®) coverage.

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    Clinical & Coding Specifications

    Clinical Context

    A typical patient for 80321 is an adult undergoing laboratory testing for alcohol exposure assessment. Common scenarios include pre-employment or workplace screening, monitoring in substance use disorder treatment programs, forensic or legal testing (e.g., probation or custody cases), and clinical evaluation when alcohol ingestion is suspected in the emergency department or inpatient setting. The clinical workflow begins with specimen collection (blood, urine, or other validated matrix) under chain-of-custody when required. The specimen is labeled and delivered to a clinical laboratory. A laboratory analyst performs the 80321 assay to detect and quantify one or two alcohol biomarker compounds (for example ethyl glucuronide [EtG] or ethyl sulfate [EtS], or phosphatidylethanol [PEth] when present), documents results in the laboratory information system, and reports findings to the ordering clinician or program. Results inform clinical decisions such as treatment modification, workplace action, or legal reporting. Typical sites of service include outpatient laboratory collection centers, hospital outpatient laboratories, inpatient hospital laboratories, and specialized forensic collection sites.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80307Drug screening, qualitative; multiple drug classes by instrumented chemistry analyzer, each procedureCommonly performed alongside 80321 when comprehensive substance use screening is required

    80305 | Drug screening, qualitative; single drug class method (e.g., immunoassay) | May be used prior to or in conjunction with 80321 for initial rapid screening

    80324 | Measurement of ethanol (blood alcohol) | Direct blood ethanol measurement is often ordered with alcohol biomarker testing to assess recent ingestion

    80366 | Drug confirmation, quantitative, by definitive method (e.g., GC/MS, LC/MSMS) | Confirmatory testing for biomarkers or positive screens may follow 80321 when quantitation or definitive identification is required

    CPT 80053 | Comprehensive metabolic panel (Note: panel code example) | Routine metabolic panels may be ordered concurrently to assess organ function in patients with suspected alcohol use

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    208D00000X
    207Q00000X
    208000000X
    173000000X
    2080T0002X
    156FX1100X
    208U00000X
    1710I1003X

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    F10.10Alcohol abuse, uncomplicated
    F10.129Alcohol abuse with intoxication, unspecified
    F10.14Alcohol abuse with alcohol-induced mood disorder
    F10.159Alcohol abuse with alcohol-induced psychotic disorder, unspecified
    F10.182Alcohol abuse with alcohol-induced sleep disorder
    F10.20Alcohol dependence, uncomplicated
    F10.21Alcohol dependence, in remission
    F10.24Alcohol dependence with alcohol-induced mood disorder
    F10.27Alcohol dependence with alcohol-induced persisting dementia
    F10.920Alcohol use, unspecified with intoxication, uncomplicated

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Hematology/Oncology1 / 2

    10 rows

    PolicyPayerEffective Date
    Hematology - AdzynmaCigna2026-10-15
    Tumor Treating Fields for Pancreatic Cancerselect_health2026-10-01
    Axicabtagene ciloleucel (Yescarta®) coverageBaylor Scott & White Health Plan2026-10-01
    Electric Tumor Treatment Field Therapy (for Kentucky Only)UnitedHealthcare2026-10-01
    Electric Tumor Treatment Field Therapy (for Kansas Only)UnitedHealthcare2026-10-01
    Electric Tumor Treatment Field Therapy (for Idaho Only)UnitedHealthcare2026-10-01
    Brexucabtagene autoleucel (Tecartus) CoverageBaylor Scott & White Health Plan2026-10-01
    Electric Tumor Treatment Field Therapy (for Pennsylvania Only)UnitedHealthcare2026-10-01
    Epithelial Cell Cytology in Breast Cancer Risk AssessmentOscar Health2026-10-01
    Factor VIII ProductsCigna2026-09-15

    Related Codes

    CPT-80320-ALCOHOL-DETECTION-IN-SPECIMEN

    CPT 80320: Alcohol Detection and Quantification

    CPT-80322-ALCOHOL-BIOMARKER-PANEL-MULTI-ANALYTE

    CPT 80322: Alcohol Biomarker Panel, Three or More Compounds

    CPT-80323-ALKALOID-DRUG-LEVEL-ANALYSIS

    CPT 80323: Alkaloid Drug Identification and Quantitation

    CPT-80324-AMPHETAMINE-DRUG-TESTING-LAB-ASSAY

    CPT 80324: Amphetamine Detection and Quantification

    CPT-80325-AMPHETAMINES-DETECTION-AND-QUANTIFICATION

    CPT 80325: Detection of Three or Four Amphetamines

    CPT-80326-AMPHETAMINES-PANEL-5-OR-MORE

    CPT 80326: Detection/Quantification of Five or More Amphetamines

    CPT-80334-SEROTONERGIC-ANTIDEPRESSANT-PANEL

    CPT 80334: Detection of Six or More Serotonergic Antidepressants

    CPT-80335-TRICYCLIC-ANTIDEPRESSANT-ASSAY

    CPT 80335: Tricyclic and Cyclical Antidepressant Assay

    CPT-80344-MULTI-ANTIPSYCHOTIC-PANEL-SEVEN-PLUS-DRUGS

    CPT 80344: Multiplex Antipsychotic Drug Detection (7+ agents)

    CPT-80345-BARBITURATE-DETECTION-SERUM-URINE

    CPT 80345: Barbiturate Detection in Serum or Urine

    Showing 1–10 of 24

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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