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    v1.0.30
    OpenPayerBilling CodesHLA TypingCPT cpt-81375-hla-class-ii-low-intermediate-resolution-typing
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    CPT 81375: HLA Class II Low/Intermediate-Resolution Molecular Typing

    CPT code 81375 describes a laboratory molecular test for low- or intermediate-resolution typing of human leukocyte antigen (HLA) Class II loci, specifically HLA–DRB1/3/4/5 and HLA–DQB1; the service is a technical laboratory assay using molecular techniques and is typically performed in a clinical or reference laboratory setting rather than at bedside or in an outpatient clinic.

    For related coverage guidance, see recent payer policy updates: Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency, Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policy.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old patient undergoing evaluation for kidney transplantation is referred for HLA Class II typing to assess compatibility with a potential living donor. The ordering clinician is a transplant nephrologist coordinating pre-transplant immunogenetic workup. The patient presents to an outpatient phlebotomy clinic where a blood sample is collected and sent to a molecular diagnostics laboratory. In the lab, a molecular technologist performs low- or intermediate-resolution HLA Class II typing using sequence-specific oligonucleotide (SSO) or sequence-specific primer (SSP) methods to determine HLA–DRB1/3/4/5 and HLA–DQB1 alleles. Results are reported to the transplant team and entered into the transplant registry for donor-recipient matching and immunologic risk assessment. Typical workflow steps include order entry, specimen collection, accessioning, DNA extraction, PCR-based molecular typing, result interpretation by the lab analyst, and transmission of a coded report to the transplant program and electronic health record. Typical site of service is an outpatient molecular diagnostic laboratory or hospital-based immunogenetics laboratory with coordination from the transplant clinic.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    81376HLA class II high-resolution typing, DRB1/3/4/5, DQB1 by molecular methodsOften performed when high-resolution allele-level typing is required after low/intermediate resolution 81375 for donor selection or desensitization planning.
    81479Unlisted molecular pathology procedureUsed for specialized or emerging HLA molecular assays not captured by existing codes when required.
    86850Blood typing; ABOPerformed as part of pre-transplant immunohematology screening alongside HLA typing.
    86308HLA antibody identification, single antigen beadComplementary testing to HLA typing to assess recipient sensitization and antibody specificity in transplant evaluation.
    88240DNA probe; other specified probeAncillary molecular methods occasionally used in comprehensive immunogenetic workups in specialized labs.

    Related Modifiers

    7 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    208000000X
    207RC0000X
    207LP2900X
    207L00000X
    1223E0200X
    146M00000X
    261QE0002X
    2279H0200X

    Related ICD-10-CM Codes

    28 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    D73.3Abscess of spleen
    D82.1Di George's syndrome
    D89.89Other specified disorders involving the immune mechanism, not elsewhere classified
    K50.90Crohn's disease, unspecified, without complications
    K51.90Ulcerative colitis, unspecified, without complications
    L40.9Psoriasis, unspecified
    L93.0Discoid lupus erythematosus
    M05.79Rheumatoid arthritis with rheumatoid factor of multiple sites without organ or systems involvement
    M06.9Rheumatoid arthritis, unspecified

    Showing 1–10 of 28

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Carrier Testing Panels for Genetic Diseases (for Kentucky Only)UnitedHealthcare2026-10-01
    Genetic Testing for Neurological Disorders (for Kansas Only)UnitedHealthcare2026-10-01
    Genetic and Molecular TestingProvidence Health Plan2026-10-01
    Cell-Free Fetal DNA Testing (for Idaho Only)UnitedHealthcare2026-10-01
    Next Generation Sequencing for Minimal Residual Disease DetectionProvidence Health Plan2026-10-01
    Pharmacogenetic Panel Testing (for Kansas Only)UnitedHealthcare2026-10-01
    Non-Covered Genetic Panel TestsProvidence Health Plan2026-10-01

    Related Codes

    CPT-81370-HLA-CLASS-I-II-LOW-INTERMEDIATE-RESOLUTION-TESTING

    CPT 81370: HLA Class I and II Low/Intermediate Resolution Typing

    CPT-81371-HLA-A-B-DRB1-LOW-INTERMEDIATE-RESOLUTION-TYPING

    CPT 81371: Low/Intermediate-Resolution HLA-A, -B, and -DRB1 Typing

    CPT-81372-HLA-CLASS-I-LOW-INTERMEDIATE-RESOLUTION-TYPING

    CPT 81372: HLA Class I Low/Intermediate Resolution Typing

    CPT-81373-HLA-CLASS-I-LOW-INTERMEDIATE-RESOLUTION

    CPT 81373: HLA Typing, Low or Intermediate Resolution, One Class I Locus

    CPT-81374-HLA-CLASS-I-LOW-INTERMEDIATE-RESOLUTION

    CPT 81374: HLA Class I Allele Group Typing, Low/Intermediate Resolution

    CPT-81376-HLA-CLASS-II-TYPING-LOW-INTERMEDIATE-RESOLUTION-TECHNICAL

    CPT 81376: HLA Class II Low/Intermediate Resolution, Technical Test

    CPT-81377-HLA-CLASS-II-ALLELE-GROUP-TYPING-LOW-INTERMEDIATE-RESOLUTION

    CPT 81377: HLA Class II Allele Group Typing, Low/Intermediate Resolution

    CPT-81378-HLA-HIGH-RESOLUTION-MOLECULAR-TYPING-CLASS-I-II-DRB1

    CPT 81378: High-Resolution HLA Class I and II Typing

    CPT-81379-HLA-CLASS-I-HIGH-RESOLUTION-TYPING

    CPT 81379: HLA Class I High-Resolution Molecular Typing

    CPT-81380-HLA-HIGH-RESOLUTION-CLASS-I-TYPING

    CPT 81380: HLA High-Resolution Molecular Typing, One Class I Locus

    Showing 1–10 of 13

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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