Loading...

    Explore
    • Policy Monitoring
    • Live Policy Feed
    • Billing Codes
    • Healthcare Providers
    • Health Insurance Payers
    • States
    • Service Lines
    • Taxonomy Codes
    Lookup tools
    • Billing Code Lookup
    • CPT Modifier Lookup
    • Provider Taxonomy Code Lookup
    • Healthcare Provider & Facility Lookup
    • Health Insurance Payer Lookup
    • Payer Contact Directory

    Policy & Benchmark Tools

    • Prior Authorization Lookup
    • Coverage Criteria Lookup
    • Timely Filing Lookup
    • Billing Code Reimbursement Rates
    • Payer Comparison Dashboard
    • Facility Comparison Dashboard
    • All tools

    Company

    • About OpenPayer
    • Data Sources
    • Trek Health
    OpenPayerby Trek Health
    LinkedInYouTube

    © 2026 Trek Health. All rights reserved.

    OpenPayer is built and maintained by Trek Health.

    v1.0.30
    OpenPayerBilling CodesReproductive Medicine ProceduresCPT cpt-89354-thawing-cryopreserved-reproductive-tissue
    Ask Trek AI about this page

    CPT 89354: Thawing of Cryopreserved Testicular or Ovarian Tissue

    CPT code 89354 describes laboratory thawing of previously cryopreserved testicular or ovarian reproductive tissue for later clinical use; this is a laboratory reproductive tissue processing service typically performed in an assisted reproductive technology (ART) laboratory or specialized fertility clinic laboratory setting and supports procedures such as tissue transplantation or use in fertility preservation workflows.

    For related coverage guidance, see recent payer policy updates: Prenatal (Fetal) Diagnostic Testing, Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency.

    Customize your policy alerts

    Sign up for cpt 89354 policy alerts

    Get alerted when payer policies referencing 89354 are released or updated.

    Monitor payer policy activity

    Receive email alerts as soon as relevant payer policies are published or updated.

    Table of contents

    Progress1 / 7

    Explore openpayer tools

    • Billing CodesCPT, HCPCS, DRG & more
    • Reimbursement RatesCommercial vs. Medicare rate reports
    • Facility ComparisonBeds, revenue & payer mix
    • ProvidersHospitals & facilities
    • PayersPlans & market footprint
    • Taxonomy CodesNUCC specialty codes
    • ModifiersCPT modifiers & usage
    • Prior AuthorizationPA policies by payer & drug
    • Timely FilingFiling deadlines by payer
    • Coverage CriteriaMedical necessity by payer
    • Payer ContactsClaims, PA & appeals contacts
    View all tools

    Clinical & Coding Specifications

    Clinical Context

    A 32-year-old patient with a prior diagnosis of chemotherapy-induced infertility requests use of previously cryopreserved testicular or ovarian tissue for assisted reproduction. The reproductive endocrinology team coordinates scheduling with the laboratory to retrieve and prepare the tissue on the day of the fertility procedure. The lab analyst receives a thaw request, confirms tissue identity and chain of custody, performs controlled-rate thawing of the cryopreserved testicular or ovarian reproductive tissue, evaluates viability, documents thaw parameters, and transfers the thawed tissue to the receiving clinical team or embryology suite for subsequent fertilization, ovarian tissue transplantation, or implantation procedures. Typical workflow steps include verification of patient and specimen identifiers, removal of cryovial(s) from long-term storage, stepwise warming and cryoprotectant removal, assessment of tissue integrity, and documentation in the laboratory information system. Typical site of service is an outpatient ambulatory surgery center or hospital-based fertility laboratory associated with an assisted reproductive technology program.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    89250Recovery of oocytes from ovaries; transvaginalOften precedes cryopreservation of ovarian tissue or follows thaw when combined with assisted reproduction.
    89258Microinjection of oocyte (intracytoplasmic sperm injection)May follow thawing when oocytes or ovarian tissue-derived oocytes are used for fertilization.
    89342Transfer of human embryos to the uterus, intrauterineMay occur after thawing and embryo preparation in the ART workflow.
    89260Assisted embryo hatching, any methodMay be performed on embryos derived from thawed gametes or tissue prior to transfer.
    89280Semen or sperm processing; preparation of specimen for intrauterine insemination (IUI)Sperm preparation commonly occurs in parallel to thawing of partner/donor tissue in ART cycles.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    55Modifier –55 indicates the billing provider furnished only postoperative management for a procedure that has a global surgical period. According to AAPC, this modifier is appended to the same CPT…- Use modifier -55 when billing the same CPT surgical code that has a 10- or 90-day global period and you, as a provider other than the original surgeon, furnished ONLY postoperative management (no…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207L00000X
    363A00000X
    332800000X
    251X00000X
    111NP0017X
    207QB0505X
    103TM1800X
    1835N1003X

    Related ICD-10-CM Codes

    22 rows

    ICD-10-CM CodeDescription
    C56.9Malignant neoplasm of unspecified ovary
    D27.9Benign neoplasm of unspecified ovary
    D28.9Benign neoplasm of female genital organ, unspecified
    E23.0Hypopituitarism
    E29.1Testicular hypofunction
    N91.2Amenorrhea, unspecified
    N97.0Female infertility associated with anovulation
    N97.1Female infertility of tubal origin
    N97.2Female infertility of uterine origin
    N97.8Female infertility of other origin

    Showing 1–10 of 22

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Prenatal (Fetal) Diagnostic TestingBlue Cross Blue Shield - Rhode Island2027-01-01
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Polygenic Risk ScoreBlue Cross Blue Shield - Rhode Island2026-12-01
    Gene Expression Profiling for Cutaneous MelanomaBlue Cross Blue Shield - Rhode Island2026-12-01
    Genetic Testing for Diagnosis and Management of Mental Health ConditionsBlue Cross Blue Shield - Rhode Island2026-11-01
    Molecular Testing in the Management of Pulmonary NodulesBlue Cross Blue Shield - Rhode Island2026-11-01
    Genetic Testing for Hereditary Cancer (for North Carolina Only)Colorado Rocky Mountain Health Plans2026-10-01
    Expanded Molecular Testing of Cancers to Select Targeted TherapiesBlue Cross Blue Shield - North Dakota2026-10-01

    Related Codes

    CPT-89250-SHORT-TERM-OOCYTE-EMBRYO-CULTURE-ART

    CPT 89250: Oocyte or Embryo Culture <4 Days

    CPT-89251-OOCYTE-EMBRYO-SHORT-TERM-COCULTURE-ART

    CPT 89251: Oocyte/Embryo Culture with Coculture (<4 days)

    CPT-89253-ASSISTED-EMBRYO-HATCHING-PARTIAL-ZONA-DISSECTION

    CPT 89253: Assisted Embryo Hatching, Partial Zona Dissection

    CPT-89254-FOLLICULAR-FLUID-OOCYTE-IDENTIFICATION-LAB

    CPT 89254: Oocyte Identification from Follicular Fluid

    CPT-89255-EMBRYO-PREPARATION-FOR-TRANSFER-ART

    CPT 89255: Embryo Preparation for Transfer (ART)

    CPT-89257-SPERM-IDENTIFICATION-FROM-ASPIRATION

    CPT 89257: Identification of Sperm from Non-Seminal Aspirates

    CPT-89258-EMBRYO-CRYOPRESERVATION-IVF

    CPT 89258: Embryo Cryopreservation During IVF Cycle

    CPT-89259-SPERM-CRYOPRESERVATION-AND-PRESERVATION

    CPT 89259: Sperm Cryopreservation, Laboratory Processing

    CPT-89260-LABORATORY-SPERM-PREPARATION-SPERM-WASH-SWIM-UP

    CPT 89260: Sperm Isolation for Sperm Wash and Swim‑Up Preparation

    CPT-89261-SPERM-PREPARATION-AND-SEMEN-ANALYSIS

    CPT 89261: Complex Sperm Preparation and Semen Analysis

    Showing 1–10 of 36

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
    Published by

    Trek Health powers OpenPayer with a leading commercial payer intelligence platform, seamlessly converting fragmented payer data into actionable insights for provider organizations.

    Trek Health ingests and normalizes Transparency in Coverage data and payer policy updates to give provider organizations a clear view of how commercial reimbursement behaves across markets, payers, and services. Our platform transforms raw payer disclosures into structured intelligence that supports contract evaluation, payer negotiations, and service line strategy. By combining market benchmarks with ongoing policy visibility, Trek helps teams identify variability, risk, and opportunity in commercial reimbursement. The result is faster insight, stronger negotiating positions, and more informed financial decisions.