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    v1.0.30
    OpenPayerBilling CodesFluid washings, Hybridization and Sex Chromatin Identification ProceduresCPT cpt-88130-sex-chromatin-barr-body-analysis
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    CPT 88130: Sex Chromatin (Barr Body) Analysis

    CPT code 88130 describes a laboratory technical test in which a lab analyst examines sex chromatin in cells to detect Barr bodies for sex determination; the service is a cytogenetic or pathology laboratory procedure (technical component) typically performed in a clinical laboratory or hospital lab setting and reported as a specimen-based microscopic or cytogenetic analysis.

    For related coverage guidance, see recent payer policy updates: Automated Ambulatory Blood Pressure Monitoring for Diagnosis of Hypertension in Patients with Elevated Office Blood Pressure, Cardiology - Ivabradine, Cardiac Computed Tomography (CCT)/Coronary Computed Tomographic Angiography (CCTA).

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    Clinical & Coding Specifications

    Clinical Context

    A cytogenetics laboratory receives a peripheral blood sample from a pediatrics clinic to evaluate a newborn with ambiguous external genitalia. The lab technician performs 88130 sex chromatin (Barr body) analysis as a technical test to stain interphase nuclei and assess the presence or absence of Barr bodies, which may help determine the likely chromosomal sex (presence of inactive X chromosomes).

    Typical workflow: the ordering clinician (pediatrician or neonatologist) requests testing to assist in sex determination. The specimen (usually blood or buccal smear) is accessioned into the lab, processed, fixed, and stained for sex chromatin. A trained laboratory analyst performs the microscopic technical component, documents slide counts and staining quality, and prepares a technical report. If a professional (interpretive) component is required, a pathologist or clinical cytogeneticist reviews slides and issues an interpretive report billed separately. Results are communicated to the ordering clinician for integration with physical exam, imaging, hormonal studies, and definitive karyotyping when indicated.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    88271Chromosome analysis, blood, 20 cells, cultured or uncultured, with banding; per cultureCommon follow-up to 88130 for definitive karyotype analysis when sex chromatin results are inconclusive or for confirmation.
    88272Chromosome analysis, blood, 40 cells, cultured with banding; per cultureExtended karyotype analysis providing increased resolution beyond sex chromatin screening.
    89320Tissue typing; HLA class I or class II (serologic or molecular methods)Occasionally ordered in complex diagnostic panels for disorders of sex development; not routine but may accompany genetic workup.
    84702Gonadal steroid assay (e.g., testosterone, estradiol)Hormone assays often performed alongside chromatin testing to assess sexual differentiation and guide management.
    88237FISH, each probe, including probe sets; tissue or cell cultureFluorescence in situ hybridization is commonly used after or instead of sex chromatin testing for targeted chromosome or sex-determining region evaluation.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207Q00000X
    207L00000X
    363A00000X
    207K00000X
    208000000X
    385HR2060X
    2080P0210X
    390200000X

    Related ICD-10-CM Codes

    25 rows

    ICD-10-CM CodeDescription
    C50.911Malignant neoplasm of unspecified site of right female breast
    C56.9Malignant neoplasm of unspecified ovary
    D47.2Monoclonal gammopathy
    D82.1Di George's syndrome
    E28.2Polycystic ovarian syndrome
    E29.1Testicular hypofunction
    E34.9Endocrine disorder, unspecified
    N80.9Endometriosis, unspecified
    N84.0Polyp of corpus uteri
    N92.6Irregular menstruation, unspecified

    Showing 1–10 of 25

    Related Payer Policy Updates

    Specialty: Cardiology1 / 3

    10 rows

    PolicyPayerEffective Date
    Automated Ambulatory Blood Pressure Monitoring for Diagnosis of Hypertension in Patients with Elevated Office Blood PressureBluecross Idaho2026-11-01
    Cardiology - IvabradineCigna2026-10-15
    Cardiac Computed Tomography (CCT)/Coronary Computed Tomographic Angiography (CCTA)univerahealthcare2026-10-15
    Percutaneous Ventricular Assist DeviceUnitedHealthcare2026-10-01
    Cardiovascular Disease Risk AssessmentOscar Health2026-10-01
    Biomarkers for Myocardial Infarction and Chronic Heart FailureOscar Health2026-10-01
    Multiple Procedure Payment Reduction for Diagnostic Cardiovascular ProceduresBlue Cross Blue Shield - Maine2026-10-01
    Left Atrial Appendage Closure Devicesexcellus_health_plan_inc2026-09-17
    Left Atrial Appendage Closure Devicesuniverahealthcare2026-09-17
    Cardiac Omnibus CodesCigna2026-09-15

    Related Codes

    CPT-88104-CYTOPATHOLOGY-SMEAR-PREP-AND-INTERPRETATION

    CPT 88104: Cytopathology Smear Preparation and Interpretation

    CPT-88106-NON-GYNECOLOGIC-CYTOPATHOLOGY-SMEAR-SIMPLE-FILTER

    CPT 88106: Cytopathology Smear of Non-Cervical Fluids

    CPT-88108-CYTOPATHOLOGY-FLUID-SMEAR-CONCENTRATION-TECHNIQUE

    CPT 88108: Cytopathology Concentration Smear Preparation and Interpretation

    CPT-88112-LIQUID-BASED-CYTOPATHOLOGY-SELECTIVE-CELLULAR-ENHANCEMENT

    CPT 88112: Selective Cellular Enhancement for Cytopathology

    CPT-88120-URINARY-TRACT-IN-SITU-HYBRIDIZATION-MORPHOMETRIC-ANALYSIS

    CPT 88120: In Situ Hybridization on Urinary Tract Specimen, 3–5 Probes

    CPT-88121-URINARY-TRACT-IN-SITU-HYBRIDIZATION-MORPHOMETRY

    CPT 88121: Urine In Situ Hybridization with 3–5 Probes

    CPT-88125-FORENSIC-CYTOPATHOLOGY-TECHNICAL-SERVICE

    CPT 88125: Cytopathology Specimen Analysis for Forensic Testing

    CPT-88140-PERIPHERAL-BLOOD-SMEAR-SEX-CHROMATIN-IDENTIFICATION

    CPT 88140: Peripheral Blood Smear for Sex Chromatin Identification

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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