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    OpenPayerBilling CodesFluid washings, Hybridization and Sex Chromatin Identification ProceduresCPT cpt-88120-urinary-tract-in-situ-hybridization-morphometric-analysis
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    CPT 88120: In Situ Hybridization on Urinary Tract Specimen, 3–5 Probes

    Medicare pays $378 on average nationally for this procedure.

    CPT code 88120 describes a technical laboratory procedure performing in situ hybridization on a urinary tract specimen using three to five molecular probes for manual morphometric analysis to evaluate the number and form of cellular structures; service type: molecular cytogenetic diagnostic test; typical site of service: clinical laboratory or hospital pathology lab.

    For related coverage guidance, see recent payer policy updates: Automated Ambulatory Blood Pressure Monitoring for Diagnosis of Hypertension in Patients with Elevated Office Blood Pressure, Cardiology - Ivabradine, Cardiac Computed Tomography (CCT)/Coronary Computed Tomographic Angiography (CCTA).

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    Clinical & Coding Specifications

    Clinical Context

    A 62-year-old patient with a history of recurrent urinary tract symptoms and a prior abnormal cytology presents for evaluation of possible urothelial carcinoma. A urine specimen is collected and sent to the pathology laboratory for molecular cytogenetic evaluation. In the laboratory, a histology/laboratory analyst performs in situ hybridization on cells from the urinary tract specimen using three to five molecular probes for manual morphometric analysis to assess copy number changes and cellular morphology. The workflow includes specimen accessioning, fixation and slide preparation, probe hybridization, fluorescence or chromogenic signal development, manual morphometric counting of signals and cellular structures, pathologist review of technical results, and generation of a technical report. Typical indications include abnormal urine cytology, hematuria with suspicion for urothelial neoplasm, surveillance after prior bladder cancer, or equivocal cytology requiring adjunct molecular testing. Typical site of service is a clinical pathology laboratory within a hospital or independent diagnostic laboratory, billed as a laboratory technical service.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    88112Cytopathology, smears, interpretation by physician (non-gynecologic)Often performed as initial urine cytology screening before reflexing to molecular in situ hybridization.
    88342Immunohistochemistry or immunocytochemistry, per specimen; initial single antibody stainMay be used on cell blocks or concurrent specimens to provide additional diagnostic information.
    88365Morphometric analysis, imaging, computer-assistedMay be used for quantitative morphometric analysis when automated imaging supplements manual counts.
    88313Special stains (e.g., cytochemical), per specimenAncillary stains on urine cell preparations can be performed alongside molecular probes for diagnostic correlation.
    88120In situ hybridization with 3–5 probes (technical component described)The primary technical molecular cytogenetic assay performed on the urinary tract specimen for which this coding guidance applies.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RH0000X
    207RP1001X
    2080P0207X
    363L00000X
    1835P2201X
    207QP0002X
    163WN1003X
    344600000X

    Related ICD-10-CM Codes

    29 rows

    ICD-10-CM CodeDescription
    C67.2Malignant neoplasm of lateral wall of bladder
    C67.3Malignant neoplasm of anterior wall of bladder
    C67.4Malignant neoplasm of posterior wall of bladder
    C67.9Malignant neoplasm of bladder, unspecified
    N10Acute pyelonephritis
    N12Tubulo-interstitial nephritis, not specified as acute or chronic
    N13.6Pyonephrosis
    N20.0Calculus of kidney
    N20.1Calculus of ureter
    N20.2Calculus of kidney with calculus of ureter

    Showing 1–10 of 29

    Related Payer Policy Updates

    Specialty: Cardiology1 / 3

    10 rows

    PolicyPayerEffective Date
    Automated Ambulatory Blood Pressure Monitoring for Diagnosis of Hypertension in Patients with Elevated Office Blood PressureBluecross Idaho2026-11-01
    Cardiology - IvabradineCigna2026-10-15
    Cardiac Computed Tomography (CCT)/Coronary Computed Tomographic Angiography (CCTA)univerahealthcare2026-10-15
    Percutaneous Ventricular Assist DeviceUnitedHealthcare2026-10-01
    Cardiovascular Disease Risk AssessmentOscar Health2026-10-01
    Biomarkers for Myocardial Infarction and Chronic Heart FailureOscar Health2026-10-01
    Multiple Procedure Payment Reduction for Diagnostic Cardiovascular ProceduresBlue Cross Blue Shield - Maine2026-10-01
    Left Atrial Appendage Closure Devicesexcellus_health_plan_inc2026-09-17
    Left Atrial Appendage Closure Devicesuniverahealthcare2026-09-17
    Cardiac Omnibus CodesCigna2026-09-15

    Related Codes

    CPT-88104-CYTOPATHOLOGY-SMEAR-PREP-AND-INTERPRETATION

    CPT 88104: Cytopathology Smear Preparation and Interpretation

    CPT-88106-NON-GYNECOLOGIC-CYTOPATHOLOGY-SMEAR-SIMPLE-FILTER

    CPT 88106: Cytopathology Smear of Non-Cervical Fluids

    CPT-88108-CYTOPATHOLOGY-FLUID-SMEAR-CONCENTRATION-TECHNIQUE

    CPT 88108: Cytopathology Concentration Smear Preparation and Interpretation

    CPT-88112-LIQUID-BASED-CYTOPATHOLOGY-SELECTIVE-CELLULAR-ENHANCEMENT

    CPT 88112: Selective Cellular Enhancement for Cytopathology

    CPT-88121-URINARY-TRACT-IN-SITU-HYBRIDIZATION-MORPHOMETRY

    CPT 88121: Urine In Situ Hybridization with 3–5 Probes

    CPT-88125-FORENSIC-CYTOPATHOLOGY-TECHNICAL-SERVICE

    CPT 88125: Cytopathology Specimen Analysis for Forensic Testing

    CPT-88130-SEX-CHROMATIN-BARR-BODY-ANALYSIS

    CPT 88130: Sex Chromatin (Barr Body) Analysis

    CPT-88140-PERIPHERAL-BLOOD-SMEAR-SEX-CHROMATIN-IDENTIFICATION

    CPT 88140: Peripheral Blood Smear for Sex Chromatin Identification

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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