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    v1.0.28
    OpenPayerBilling CodesQualitative or Semiquantitative ImmunoassaysCPT cpt-86226-ssdna-antibody-assay-serologic
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    CPT 86226: ssDNA Antibody Assay, Serum

    CPT code 86226 describes a laboratory immunoassay performed by a lab analyst to detect antibodies to single-stranded DNA (ssDNA) in a patient’s serum, a clinical serology test commonly used in autoimmune evaluation; service type: laboratory serology assay, typical site of service: clinical laboratory or hospital lab.

    For related coverage guidance, see recent payer policy updates: Diagnosis of Idiopathic Environmental Intolerance, Allergen Testing, Allergen Testing.

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    Table of contents

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    • Billing CodesCPT, HCPCS, DRG & more
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    Clinical & Coding Specifications

    Clinical Context

    A 34-year-old female with a history of systemic lupus erythematosus (SLE) presents to the outpatient rheumatology clinic with increased joint pain, fatigue, and a recent low-grade fever. The clinician orders autoimmune serology to assess disease activity and clarify serologic markers. A venous blood specimen is collected in the clinic phlebotomy area and sent to the hospital or reference laboratory. The laboratory analyst performs an assay to detect antibodies to single-stranded DNA (ssDNA) in the patient’s serum using immunoassay or other validated serologic techniques. Results are reported to the ordering rheumatologist and incorporated into the clinical assessment of autoimmune disease activity and differential diagnosis. Typical site of service: outpatient clinic or clinical laboratory. Service type: laboratory diagnostic serologic test.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80101Therapeutic drug assay, qualitative or quantitative; multiple drugs, multiple classesMay be ordered in parallel when monitoring medication levels in autoimmune patients (e.g., immunosuppressants).
    86038Antinuclear antibody (ANA), titer and patternCommonly ordered alongside 86226 as an initial screening test for systemic autoimmune diseases.
    86255Antibodies to double-stranded DNA (dsDNA)Frequently ordered with ssDNA antibodies to further characterize anti-DNA reactivity in SLE and related disorders.
    86431Antibodies to extractable nuclear antigens (ENA) panelOften performed in conjunction to identify specific autoantibodies associated with connective tissue diseases.
    99000Handling and/or conveyance of specimenAdministrative/handling code sometimes used for special specimen transport to reference labs where 86226 may be sent.

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207RH0000XHematology (Internal Medicine) PhysicianAn internist with additional training who specializes in diseases of the blood, spleen and lymph. This specialist treats conditions such as anemia, cl…
    208M00000XHospitalist PhysicianHospitalists are physicians whose primary professional focus is the general medical care of hospitalized patients. Their activities include patient ca…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    363L00000XNurse Practitioner(1) A registered nurse provider with a graduate degree in nursing prepared for advanced practice involving independent and interdependent decision mak…
    385HR2065XChild Physical Disabilities Respite CareA facility or distinct part of a facility that providers short term, residential care to children, diagnosed with complex or profound disabilities as …
    174400000XSpecialistAn individual educated and trained in an applied knowledge discipline used in the performance of work at a level requiring knowledge and skills beyond…
    2472R0900XRenal Dialysis TechnicianTechnologists, Technicians & Other Technical Service Providers · Technician, Other

    Related ICD-10-CM Codes

    26 rows

    ICD-10-CM CodeDescription
    D68.9Coagulation defect, unspecified
    D69.6Thrombocytopenia, unspecified
    E10.9Type 1 diabetes mellitus without complications
    E11.9Type 2 diabetes mellitus without complications
    E78.5Hyperlipidemia, unspecified
    F45.41Pain disorder exclusively related to psychological factors
    I10Essential (primary) hypertension
    J98.4Other disorders of lung
    L93.0Discoid lupus erythematosus
    M05.9Rheumatoid arthritis with rheumatoid factor, unspecified

    Showing 1–10 of 26

    Related Payer Policy Updates

    Specialty: Allergy/Clinical Immunology1 / 3

    10 rows

    PolicyPayerEffective Date
    Diagnosis of Idiopathic Environmental IntoleranceOscar Health2026-10-01
    Allergen Testingblue cross blue shield - louisiana2026-09-15
    Allergen TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Hereditary Angioedema - EkterlyCigna2026-08-15
    Omalizumab products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab Products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab ProductsPremera Bluecross2026-08-01
    Allergy Testinguniverahealthcare2026-07-16
    Omalizumab (Xolair) and related biologics coverageMass General Brigham Health Plan2026-07-01
    Xolair (omalizumab) (Subcutaneous) — Concomitant therapy restrictions and dosing guidanceeocco2026-07-01

    Related Codes

    86803-QUALITATIVE-OR-SEMIQUANTITATIVE-IMMUNOASSAYS-HEPATITIS-C

    CPT 86803: Hepatitis C Antibody Immunoassay in Serum

    CPT-86000-FEBRILE-AGGLUTININS-SEROLOGIC-TEST

    CPT 86000: Febrile Agglutinins (Serologic Agglutination) Test

    CPT-86001-ALLERGEN-SPECIFIC-IGG-LABORATORY-TEST

    CPT 86001: Allergen-Specific IgG Assay, Technical Component

    CPT-86003-ALLERGEN-SPECIFIC-IGE-ASSAY-TECHNICAL

    CPT 86003: Allergen‑Specific IgE Assay Using Crude Allergen Extract

    CPT-86005-MULTI-ALLERGEN-IGE-SCREENING-QUALITATIVE

    CPT 86005: Multi-Allergen Allergen-Specific IgE Screening

    CPT-86008-ALLERGEN-COMPONENT-SPECIFIC-IGE-ASSAY

    CPT 86008: Allergen Component–Specific IgE Assay

    CPT-86015-ANTI-ACTIN-SMOOTH-MUSCLE-ANTIBODY-ASMA

    CPT 86015: Actin (Smooth Muscle) Antibody (ASMA) Immunoassay

    CPT-86021-LEUKOCYTE-ANTIBODY-IDENTIFICATION-LAB-TECHNICAL

    CPT 86021: Leukocyte Antibody Identification Laboratory Test

    CPT-86022-PLATELET-ANTIBODY-IDENTIFICATION-TECHNICAL-TEST

    CPT 86022: Platelet Antibody Detection and Identification

    CPT-86023-PLATELET-ASSOCIATED-IMMUNOGLOBULIN-DETECTION

    CPT 86023: Platelet-Associated Immunoglobulin Assay

    Showing 1–10 of 199

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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