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    v1.0.28
    OpenPayerBilling CodesQualitative or Semiquantitative ImmunoassaysCPT cpt-86325-immunoelectrophoresis-body-fluid-immunoglobulin-analysis
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    CPT 86325: Immunoelectrophoresis of Body Fluid with Concentration

    Medicare pays $18 on average nationally for this procedure.

    CPT code 86325 describes immunoelectrophoresis (IEP) performed on a body fluid other than serum (for example, urine or cerebrospinal fluid) with concentration to evaluate the amounts and types of immunoglobulins (antibodies); this is a laboratory diagnostic procedure used to identify and characterize protein/antibody patterns, typically billed as a laboratory service and usually performed in a clinical laboratory or hospital laboratory setting.

    For related coverage guidance, see recent payer policy updates: Diagnosis of Idiopathic Environmental Intolerance, Allergen Testing, Allergen Testing.

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    Clinical & Coding Specifications

    Clinical Context

    A 62-year-old patient with a history of unexplained neurologic symptoms and episodic headaches presents with concern for possible central nervous system inflammation or monoclonal gammopathy. The treating neurologist collects cerebrospinal fluid (CSF) via lumbar puncture and sends a concentrated CSF specimen to the clinical laboratory for immunoelectrophoresis to evaluate immunoglobulin types and patterns. The laboratory analyst performs 86325 (immunoelectrophoresis of body fluid other than serum, with concentration) to detect oligoclonal bands or abnormal immunoglobulin production. Results are used by the neurologist and consulting specialists to support diagnoses such as multiple sclerosis, CNS infection, or leptomeningeal malignancy and to guide further testing (e.g., serum/CSF paired studies) and management decisions. Typical site of service is an outpatient hospital laboratory or independent clinical laboratory; specimen collection occurs in an ambulatory clinic, emergency department, or inpatient setting prior to laboratory testing. The workflow includes specimen collection and labeling, concentration of the non-serum body fluid (for example, CSF or urine), performance of immunoelectrophoresis by a trained lab technologist, interpretation and reporting by laboratory staff, and transmission of results to the ordering clinician.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    36415Collection of venous blood by venipunctureOften performed prior to or concurrent with body fluid collection to obtain serum for paired comparisons with 86325.
    62270Spinal puncture, lumbar; diagnosticProcedure used to obtain cerebrospinal fluid specimen that may be sent for 86325.
    84157Albumin; urine (qualitative or quantitative)Urine/CSF protein testing that may be ordered alongside immunoelectrophoresis for protein quantitation.
    83970Protein; total, direct measurementMay be performed on the same specimen to quantify total protein before electrophoretic separation.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    208000000XPediatrics PhysicianA pediatrician is concerned with the physical, emotional and social health of children from birth to young adulthood. Care encompasses a broad spectru…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    363LP0200XPediatric Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner
    225X00000XOccupational TherapistAn occupational therapist is a person who has graduated from an entry-level occupational therapy program accredited by the Accreditation Council for O…
    261QC0050XCritical Access Hospital Clinic/CenterAn outpatient entity, facility, or distinct part of a facility within or affiliated with a Critical Access Hospital that provides access to primary ca…
    226300000XKinesiotherapistA provider trained and educated in the applied science of medically prescribed therapeutic exercise, education and adapted physical activities designe…
    163WX0800XOrthopedic Registered NurseNursing Service Providers · Registered Nurse

    Related Payer Policy Updates

    Specialty: Allergy/Clinical Immunology1 / 3

    9 rows

    PolicyPayerEffective Date
    Diagnosis of Idiopathic Environmental IntoleranceOscar Health2026-10-01
    Allergen Testingblue cross blue shield - louisiana2026-09-15
    Allergen TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Hereditary Angioedema - EkterlyCigna2026-08-15
    Omalizumab products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab Products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab ProductsPremera Bluecross2026-08-01
    Allergy Testinguniverahealthcare2026-07-16
    Omalizumab (Xolair) and related biologics coverageMass General Brigham Health Plan2026-07-01

    Related Codes

    86803-QUALITATIVE-OR-SEMIQUANTITATIVE-IMMUNOASSAYS-HEPATITIS-C

    CPT 86803: Hepatitis C Antibody Immunoassay in Serum

    CPT-86000-FEBRILE-AGGLUTININS-SEROLOGIC-TEST

    CPT 86000: Febrile Agglutinins (Serologic Agglutination) Test

    CPT-86001-ALLERGEN-SPECIFIC-IGG-LABORATORY-TEST

    CPT 86001: Allergen-Specific IgG Assay, Technical Component

    CPT-86003-ALLERGEN-SPECIFIC-IGE-ASSAY-TECHNICAL

    CPT 86003: Allergen‑Specific IgE Assay Using Crude Allergen Extract

    CPT-86005-MULTI-ALLERGEN-IGE-SCREENING-QUALITATIVE

    CPT 86005: Multi-Allergen Allergen-Specific IgE Screening

    CPT-86008-ALLERGEN-COMPONENT-SPECIFIC-IGE-ASSAY

    CPT 86008: Allergen Component–Specific IgE Assay

    CPT-86015-ANTI-ACTIN-SMOOTH-MUSCLE-ANTIBODY-ASMA

    CPT 86015: Actin (Smooth Muscle) Antibody (ASMA) Immunoassay

    CPT-86021-LEUKOCYTE-ANTIBODY-IDENTIFICATION-LAB-TECHNICAL

    CPT 86021: Leukocyte Antibody Identification Laboratory Test

    CPT-86022-PLATELET-ANTIBODY-IDENTIFICATION-TECHNICAL-TEST

    CPT 86022: Platelet Antibody Detection and Identification

    CPT-86023-PLATELET-ASSOCIATED-IMMUNOGLOBULIN-DETECTION

    CPT 86023: Platelet-Associated Immunoglobulin Assay

    Showing 1–10 of 199

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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