CPT code 86225 represents a laboratory immunology assay to detect native or double‑stranded DNA (dsDNA) antibodies in patient serum. This test is clinically important for the evaluation and monitoring of autoimmune diseases where anti‑dsDNA antibodies are relevant, and it is commonly ordered by rheumatology, nephrology, and primary care clinicians. Nationally, CPT code 86225 is a standard laboratory service used across hospital and independent clinical laboratory settings and factors into utilization and laboratory billing patterns.

Key payers in this analysis include Aetna, Blue Cross Blue Shield, Cigna Health, UnitedHealthcare, and Medicare. Readers will find an overview of the clinical context for the assay, typical sites of service, common modifiers associated with laboratory billing, and the payers that commonly cover the test. The publication summarizes benchmark pricing and utilization considerations, highlights relevant billing and coding nuances for laboratories, and outlines where to find supporting clinical and billing resources.

This summary is intended to provide a concise national view of CPT code 86225, helping clinical laboratory managers, billing professionals, and policy analysts understand the code’s clinical purpose, typical use settings, and payer landscape. Data not available in the input is noted where applicable.