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    v1.0.28
    OpenPayerBilling CodesTherapeutic Drug AssaysCPT cpt-80358-methadone-detection-or-quantification
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    CPT 80358: Methadone Detection in Patient Specimen

    CPT code 80358 describes a laboratory test in which the analyst measures the amount of or detects the presence of methadone in a patient specimen; the service type is toxicology/drug testing and the typical site of service is a clinical laboratory or hospital laboratory where specimens (urine, blood, or other validated sample types) are analyzed for methadone presence or concentration.

    For related coverage guidance, see recent payer policy updates: Epithelial Cell Cytology in Breast Cancer Risk Assessment, Chemotherapy Observation or Inpatient Hospitalization (for New Mexico Only), Umbilical Cord Blood Harvesting and Storage for Future Use (for Indiana Only).

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    Table of contents

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    • Billing CodesCPT, HCPCS, DRG & more
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    Clinical & Coding Specifications

    Clinical Context

    A typical patient is an adult enrolled in an outpatient addiction treatment program or a pain management clinic who is prescribed methadone for opioid use disorder or chronic pain. The clinician orders a laboratory urine or serum drug test to measure methadone concentration or to confirm presence for monitoring therapeutic compliance, detecting diversion, or assessing potential toxicity. Specimen collection occurs at a clinic or outpatient laboratory; the specimen is sent to a clinical chemistry/toxicology laboratory where a laboratory analyst performs an assay (e.g., immunoassay screening followed by confirmatory chromatographic testing) and documents quantitative or qualitative methadone results in the laboratory information system. Results are reviewed by the treating clinician to guide dosing, counseling, or treatment adjustments. Typical sites of service include hospital outpatient laboratories, independent clinical reference laboratories, and outpatient drug testing facilities.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80305Drug assay, presumptive, any number of drug classes; any number of devices or procedures, capable of being read by direct optical observation (e.g., dipsticks, cups, cards, cartridges), each drug classCommonly used as an initial presumptive screen before or alongside methadone-specific testing
    80307Drug assay, presumptive, any number of drug classes by instrumented chemistry analyzers, each drug classAlternative screening method performed prior to confirmatory methadone measurement
    80306Drug assay, presumptive, any number of drug classes by instrumented chemistry analyzers, multiple drug classesUsed for multi-analyte panels that may include methadone metabolites
    80358Drug test for methadoneThe specific confirmatory or quantitative methadone assay performed by the laboratory analyst
    80359Drug test, other opioid or metabolite (e.g., buprenorphine, oxycodone), quantitative or qualitativePerformed alongside 80358 when broader opioid monitoring is required
    G0480Drug testing, definitive, any number of drug classes, confirmation by definitive methods, each drug classUsed when a definitive confirmatory test (e.g., GC-MS/LC-MS/MS) is required for methadone confirmation

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207Q00000XFamily Medicine PhysicianFamily Medicine is the medical specialty which is concerned with the total health care of the individual and the family. It is the specialty in breadt…
    208D00000XGeneral Practice PhysicianA physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
    163W00000XRegistered Nurse(1) A registered nurse is a person qualified by graduation from an accredited nursing school (depending upon schooling, a registered nurse may receive…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    273100000XEpilepsy Hospital UnitAn Epilepsy Unit is a distinct unit of a hospital that provides services that may include observation, urgent care, diagnostic testing, treatment, and…
    261QH0100XHealth Service Clinic/CenterAmbulatory Health Care Facilities · Clinic/Center
    225800000XRecreation TherapistA recreation therapist uses recreational activities for intervention in some physical, social or emotional behavior to bring about a desired change in…
    3336L0003XLong Term Care PharmacyA pharmacy that dispenses medicinal preparations delivered to patients residing within an intermediate or skilled nursing facility, including intermed…

    Related ICD-10-CM Codes

    25 rows

    ICD-10-CM CodeDescription
    E66.9Obesity, unspecified
    F11.10Opioid abuse, uncomplicated
    F11.120Opioid abuse with intoxication, uncomplicated
    F11.129Opioid abuse with intoxication, unspecified
    F11.14Opioid abuse with opioid-induced mood disorder
    F11.20Opioid dependence, uncomplicated
    F11.21Opioid dependence, in remission
    F11.220Opioid dependence with intoxication, uncomplicated
    F11.229Opioid dependence with intoxication, unspecified
    F11.23Opioid dependence with withdrawal

    Showing 1–10 of 25

    Related Payer Policy Updates

    Specialty: Hematology/Oncology1 / 2

    10 rows

    PolicyPayerEffective Date
    Epithelial Cell Cytology in Breast Cancer Risk AssessmentOscar Health2026-10-01
    Chemotherapy Observation or Inpatient Hospitalization (for New Mexico Only)UnitedHealthcare2026-08-01
    Umbilical Cord Blood Harvesting and Storage for Future Use (for Indiana Only)UnitedHealthcare2026-08-01
    Antibody-Drug ConjugatesPremera Bluecross2026-08-01
    Chimeric Antigen Receptor (CAR) T-Cell TherapyColorado Rocky Mountain Health Plans2026-08-01
    Allogeneic Hematopoietic Progenitor Cell TherapyAmbetter Georgia2026-08-01
    Umbilical Cord Blood Harvesting and Storage for Future Use (for Idaho Only)UnitedHealthcare2026-08-01
    Umbilical Cord Blood Harvesting and Storage for Future Use (for New Jersey Only)UnitedHealthcare2026-08-01
    Umbilical Cord Blood Harvesting and Storage for Future Use (for Ohio Only)UnitedHealthcare2026-08-01
    Reblozyl (luspatercept-aamt) Medical Benefit Drug PolicyUnitedHealthcare2026-08-01

    Related Codes

    CPT-80143-ACETAMINOPHEN-QUANTITATIVE-SERUM-ASSAY

    CPT 80143: Acetaminophen Quantitative Assay

    CPT-80145-ADALIMUMAB-LEVEL-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80145: Adalimumab Quantitative Drug Level Assay

    CPT-80150-AMIKACIN-QUANTITATIVE-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80150: Amikacin Quantitative Serum Level

    CPT-80151-AMIODARONE-QUANTITATIVE-SERUM-ASSAY

    CPT 80151: Amiodarone Quantitative Drug Level Assay

    CPT-80155-CAFFEINE-LEVEL-TESTING

    CPT 80155: Caffeine Level Quantification

    CPT-80156-CARBAMAZEPINE-TOTAL-LEVEL-LAB-ASSAY

    CPT 80156: Carbamazepine Total Level (Serum)

    CPT-80157-FREE-CARBAMAZEPINE-ASSAY

    CPT 80157: Free Carbamazepine Quantitative Assay

    CPT-80158-CYCLOSPORINE-QUANTITATIVE-ASSAY-LAB

    CPT 80158: Cyclosporine Quantitation, Laboratory

    CPT-80159-CLOZAPINE-LEVEL-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80159: Clozapine Level Measurement

    CPT-80161-CARBAMAZEPINE-EPOXIDE-QUANTITATIVE-ASSAY

    CPT 80161: Carbamazepine 10,11-Epoxide Level, Quantitative

    Showing 1–10 of 87

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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