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    v1.0.30
    OpenPayerBilling CodesTherapeutic Drug AssaysCPT cpt-80143-acetaminophen-quantitative-serum-assay
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    CPT 80143: Acetaminophen Quantitative Assay

    CPT code 80143 describes a laboratory test in which a clinical laboratory performs the technical analysis to quantify acetaminophen concentration in a patient specimen (for example, serum); this is a toxicology/pharmacology quantitative assay typically performed as a clinical laboratory service in an outpatient or hospital laboratory setting to assess for therapeutic levels or potential overdose.

    For related coverage guidance, see recent payer policy updates: Long-Acting Injectable Antiretroviral Agents for HIV, B-Hemolytic Streptococcus Testing, Human Immunodeficiency Virus (HIV) — Coverage Criteria for Testing and Resistance Assays.

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    Clinical & Coding Specifications

    Clinical Context

    A patient arrives to the emergency department after an accidental or intentional medication ingestion and reports nausea, vomiting, and abdominal discomfort. The treating clinician obtains a medication history that suggests possible recent acetaminophen (paracetamol) overdose or uncertain timing/dose. Blood is drawn for stat toxicology testing, including a quantitative acetaminophen level to guide risk assessment and eligibility for N-acetylcysteine antidote therapy. The specimen (typically serum) is routed to the clinical laboratory. A medical laboratory scientist or clinical chemist performs the analytic assay to quantify acetaminophen, documents the result in the laboratory information system, and communicates critical values to the treating clinician. Typical site of service includes hospital inpatient laboratories, hospital emergency departments (phlebotomy or stat lab), and independent clinical laboratories receiving serum specimens. The service is a technical laboratory procedure performed by the lab analyst and reported for reimbursement under the quantitative acetaminophen assay code.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80143Acetaminophen; quantitativePrimary technical assay that quantifies acetaminophen in serum/plasma for overdose evaluation.
    80307Drug screen, qualitative; other drug classes (e.g., synthetic cannabinoids)Often ordered alongside acetaminophen for broader toxicology screening in overdose workups.
    80305Drug screen, qualitative; multiple drug classesUsed for initial toxicology screening when multiple agents are suspected.
    84148Bilirubin; totalOrdered in liver injury workup when acetaminophen toxicity is suspected to assess hepatic function.
    84460Thyroxine; total or free (T4)Not directly related but examples of other biochemical assays performed in hospital labs (included only when clinically relevant panels are ordered).
    82043Drug confirmation, specific drug, quantitativeUsed when confirmatory quantitative testing for a specific drug (other than acetaminophen) is required.

    Note: If any listed related CPT codes are not applicable in a local workflow, standard laboratory and toxicology panels will determine which adjunct tests are performed.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207Q00000X
    207R00000X
    208M00000X
    363LF0000X
    207RR0500X
    163WC1600X
    207NS0135X
    261QR0206X

    Related ICD-10-CM Codes

    29 rows

    ICD-10-CM CodeDescription
    E86.0Dehydration
    F10.129Alcohol abuse with intoxication, unspecified
    F11.20Opioid dependence, uncomplicated
    G40.909Epilepsy, unspecified, not intractable, without status epilepticus
    G93.40Encephalopathy, unspecified
    I63.9Cerebral infarction, unspecified
    R42Dizziness and giddiness
    R50.9Fever, unspecified
    R65.21Severe sepsis with septic shock
    R73.9Hyperglycemia, unspecified

    Showing 1–10 of 29

    Related Payer Policy Updates

    Specialty: Infectious Disease1 / 3

    10 rows

    PolicyPayerEffective Date
    Long-Acting Injectable Antiretroviral Agents for HIVColorado Rocky Mountain Health Plans2026-10-01
    B-Hemolytic Streptococcus TestingOscar Health2026-10-01
    Human Immunodeficiency Virus (HIV) — Coverage Criteria for Testing and Resistance AssaysOscar Health2026-10-01
    Hepatitis Testing (A, B, C, D) Coverage CriteriaOscar Health2026-10-01
    Lyme Disease Testing Coverage CriteriaOscar Health2026-10-01
    Pathogen Panel TestingOscar Health2026-10-01
    Diagnostic Testing of Common Sexually Transmitted InfectionsOscar Health2026-10-01
    Testing for Vector-Borne InfectionsOscar Health2026-10-01
    Testing for Diagnosis of Active or Latent TuberculosisOscar Health2026-10-01
    Coronavirus Testing in the Outpatient SettingOscar Health2026-10-01

    Related Codes

    CPT-80145-ADALIMUMAB-LEVEL-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80145: Adalimumab Quantitative Drug Level Assay

    CPT-80150-AMIKACIN-QUANTITATIVE-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80150: Amikacin Quantitative Serum Level

    CPT-80151-AMIODARONE-QUANTITATIVE-SERUM-ASSAY

    CPT 80151: Amiodarone Quantitative Drug Level Assay

    CPT-80155-CAFFEINE-LEVEL-TESTING

    CPT 80155: Caffeine Level Quantification

    CPT-80156-CARBAMAZEPINE-TOTAL-LEVEL-LAB-ASSAY

    CPT 80156: Carbamazepine Total Level (Serum)

    CPT-80157-FREE-CARBAMAZEPINE-ASSAY

    CPT 80157: Free Carbamazepine Quantitative Assay

    CPT-80158-CYCLOSPORINE-QUANTITATIVE-ASSAY-LAB

    CPT 80158: Cyclosporine Quantitation, Laboratory

    CPT-80159-CLOZAPINE-LEVEL-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80159: Clozapine Level Measurement

    CPT-80161-CARBAMAZEPINE-EPOXIDE-QUANTITATIVE-ASSAY

    CPT 80161: Carbamazepine 10,11-Epoxide Level, Quantitative

    CPT-80162-DIGOXIN-TOTAL-THERAPEUTIC-DRUG-MONITORING

    CPT 80162: Total Digoxin Level in Serum

    Showing 1–10 of 87

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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