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    v1.0.30
    OpenPayerBilling CodesIntroduction Procedures on the Corpus UteriCPT cpt-58323-sperm-preparation-for-insemination
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    CPT 58323: Sperm Preparation for Insemination

    Medicare pays $15 on average nationally for this procedure.

    CPT code 58323 describes laboratory preparation of sperm for insemination, a sperm washing/processing service performed to remove debris, antibodies, bacteria, and abnormal sperm prior to intrauterine or other assisted insemination; the service is a clinical laboratory reproductive procedure typically provided in an andrology or fertility clinic laboratory setting as part of assisted reproductive technology workflows.

    For related coverage guidance, see recent payer policy updates: Abortion Services, Prolonged Services for Labor Management, Prolonged Services for Labor Management.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 34-year-old woman and her partner present to a fertility clinic after 12 months of unsuccessful attempts to conceive. Semen analysis from the male partner shows suboptimal motility and the presence of debris and leukocytes. The reproductive endocrinologist orders sperm preparation for intrauterine insemination (IUI). The male partner provides a fresh semen specimen at the clinic on the day of the procedure. In the andrology laboratory, a trained laboratory technologist performs sperm washing and processing to remove seminal plasma, debris, bacteria, antisperm antibodies, and immotile or abnormal sperm, concentrating motile sperm for insemination.

    The typical workflow includes patient arrival and consent, specimen collection and labeling, initial semen analysis, sperm processing using density gradient centrifugation or swim-up techniques, post-processing analysis of concentration and motility, and preparation of the specimen for transport to the procedure room for IUI. The typical site of service is an outpatient fertility clinic or an andrology/laboratory within a reproductive endocrinology practice or ambulatory surgical center. The service type is laboratory-based assisted reproductive technology (sperm preparation) provided the same day as insemination.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    58322Injection of semen into the cervical canal; intracervical insemination (ICI)May be performed the same day as sperm preparation when ICI is the planned insemination technique following processing
    58350Interruption of pregnancy (Not applicable)Data not relevant to sperm preparation; not performed in routine fertility workflows
    89250Semen analysis; presence and motility of sperm, with microscopic examinationOften performed before and after sperm preparation to document baseline parameters and post-wash concentration and motility
    89258Sperm wash for intrauterine insemination — Note: 58323 specifically describes sperm preparation for inseminationPerformed as part of the andrology lab workflow; some labs may report adjunct semen processing and post-wash analyses with these lab CPTs
    89420Sperm capacitation by swim-up technique (assisted reproductive technique)Alternative or adjunct sperm processing technique commonly used to prepare motile sperm for IUI
    89340Sperm count, motility and morphology (complete semen analysis)Baseline and post-processing semen assessments that support and document the effectiveness of 58323

    Related Modifiers

    11 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    66Modifier 66 (Surgical Team) is used when three or more surgeons act as primary surgeons on a single, highly complex procedure. The AMA defines it for use when a surgical team — often composed of…- Use modifier 66 when three or more surgeons act as primary surgeons working collaboratively as a surgical team on a single, highly complex or difficult procedure (for example organ…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Showing 1–10 of 11

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    103K00000X
    213E00000X
    124Q00000X
    2279G0305X
    156FX1100X
    207ZP0213X
    367500000X
    2086S0105X

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    E23.0Hypopituitarism
    E29.1Testicular hypofunction
    E29.9Testicular dysfunction, unspecified
    F52.8Other sexual dysfunction not due to a substance or known physiological condition
    N46.9Male infertility, unspecified
    N48.1Balanitis
    N50.1Vascular disorders of male genital organs
    N70.01Acute salpingitis
    N71.0Acute inflammatory disease of uterus
    N71.1Chronic inflammatory disease of uterus

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Obstetrics/Gynecology1 / 2

    10 rows

    PolicyPayerEffective Date
    Abortion ServicesNew Mexico Health Care Authority2027-01-01
    Prolonged Services for Labor ManagementPremera Bluecross2027-01-01
    Prolonged Services for Labor ManagementPremera Bluecross2027-01-01
    Obstetrical Home Care ProgramsAmbetter Georgia2026-11-01
    Maternal Serum Testing for Prediction of Adverse Obstetric OutcomesPremera Bluecross2026-10-01
    Maternal Serum Testing for Prediction of Adverse Obstetric OutcomesPremera Bluecross2026-10-01
    Maternal Serum Testing for Prediction of Adverse Obstetric OutcomesBlue Cross Blue Shield - Massachusetts2026-10-01
    Cervical Cancer ScreeningOscar Health2026-10-01
    Prenatal Testing for Fetal AneuploidyOscar Health2026-10-01
    Diagnosis of VaginitisOscar Health2026-10-01

    Related Codes

    CPT-58300-IUD-INSERTION-CONTRACEPTIVE-DEVICE-PLACEMENT

    CPT 58300: Insertion of Contraceptive Intrauterine Device

    CPT-58301-IUD-REMOVAL

    CPT 58301: Removal of Intrauterine Contraceptive Device

    CPT-58321-INTRACERVICAL-INSEMINATION-PREPARED-SPERM

    CPT 58321: Intra-cervical Insemination, Insertion of Prepared Sperm

    CPT-58322-INTRAUTERINE-INSEMINATION-IUI-INSERTION-OF-PREPARED-SPERM

    CPT 58322: Intrauterine Insemination with Prepared Sperm

    CPT-58340-UTERINE-FALLOPIAN-TUBE-CONTRAST-OR-SALINE-CATHETERIZATION

    CPT 58340: Uterine and Fallopian Tube Catheterization with Contrast

    CPT-58345-TRANSCERVICAL-TUBAL-CATHETERIZATION-INFERTILITY

    CPT 58345: Fallopian Tube Catheterization with Hysterosalpingogram

    CPT-58346-HEYMAN-CAPSULE-UTERINE-BRACHYTHERAPY-INSERTION

    CPT 58346: Insertion of Heyman Capsules for Uterine Brachytherapy

    CPT-58350-CHROMOTUBATION-FALLOPIAN-TUBE-PATENCY-ASSESSMENT

    CPT 58350: Chromotubation (Fallopian Tube Patency Test)

    CPT-58353-THERMAL-ENDOMETRIAL-ABLATION-WITHOUT-HYSTEROSCOPY

    CPT 58353: Thermal Endometrial Ablation Without Hysteroscopic Guidance

    CPT-58356-ENDOMETRIAL-CRYOABLATION-UTERINE-BLEEDING

    CPT 58356: Endometrial Cryodestruction with Ultrasound Guidance

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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