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    v1.0.30
    OpenPayerBilling CodesExcision Procedures on the Femur (Thigh Region) and Knee JointCPT cpt-27330-knee-synovial-biopsy-surgical-diagnostic
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    CPT 27330: Open Biopsy of Synovium of Knee

    Medicare pays $422 on average nationally for this procedure.

    CPT code 27330 describes an open surgical synovial biopsy of the knee, in which the provider makes an incision over the knee joint and obtains synovium for diagnostic evaluation; the service type is open surgical biopsy and the typical site of service is an operating room or procedure suite with the patient under appropriate anesthesia for a knee procedure.

    For related coverage guidance, see recent payer policy updates: Removal of Implantable, Outpatient Surgical Procedures - Site of Service, Surgical Procedures.

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    Table of contents

    Progress1 / 7

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    Clinical & Coding Specifications

    Clinical Context

    A 46-year-old patient presents with chronic knee pain, swelling, and intermittent locking despite conservative management. Imaging shows persistent joint effusion and synovial thickening suggestive of inflammatory arthritis or synovial neoplasm. The orthopedic surgeon schedules a diagnostic open synovial biopsy of the knee to obtain tissue for histopathology and microbiology. The patient receives preoperative evaluation, informed consent, and regional or general anesthesia in an ambulatory surgical center or hospital operating room. Intraoperatively, a longitudinal incision is made over the affected knee, representative synovium is excised and sent for pathology, hemostasis is achieved, and the incision is closed. Postoperative care includes analgesia, wound instructions, pathology review, and follow-up to discuss results and finalize diagnosis and further management.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    29881Arthroscopy, knee, surgical; synovectomy (separate procedure)Minimally invasive alternative to open synovial biopsy for tissue sampling and debridement

    20610 | Arthrocentesis, aspiration and/or injection; major joint or bursa (e.g., shoulder, hip, knee) with or without ultrasound guidance | Diagnostic joint aspiration often performed preoperatively to obtain synovial fluid for culture, cell count, crystals prior to biopsy

    27331 | Excision or biopsy, deep soft tissue of knee (e.g., synovium) — Note: if listed in payer guides as adjunct code | Related per anatomic site; verify payer policy when performed with 27330

    99024 | Postoperative follow-up visit global period, for routine postoperative follow-up | Represents routine global postoperative care after the biopsy

    88305 | Level IV surgical pathology, gross and microscopic examination | Typical primary pathology CPT code used to report histologic evaluation of synovial biopsy specimens

    Related Modifiers

    10 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    23Modifier -23 (Unusual Anesthesia) is used on anesthesia service CPT codes when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia…- Use modifier -23 only on anesthesia CPT codes (00100–01999) when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia because of…
    50Modifier –50 (Bilateral Procedure) is appended to a CPT code when an identical procedure is performed on paired anatomical structures during the same operative session. The AMA states to add…- Use modifier -50 when the same CPT surgical, diagnostic, or radiology procedure is performed on paired anatomical structures (both sides of the body) during the same operative/session by the same…
    51Modifier –51 (Multiple Procedures) is used when the same provider performs multiple procedures in the same session to identify secondary, non‑exempt procedures and indicate payment reduction logic…- Use modifier -51 when the same provider performs multiple distinct procedures during the same patient encounter: report the highest‑valued procedure first (no modifier) and append -51 to…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    63Modifier‑63 is appended to surgical CPT codes (20000–69990) when a procedure is performed on a neonate or infant weighing ≤ 4 kg (about 8.8 lbs). The AMA/AAPC definition specifies this use and notes…- Use modifier -63 only for surgical procedures in the CPT 20000–69990 series performed on a neonate or infant who weighs ≤ 4 kg at the time of the procedure; it indicates increased complexity due to…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    81Modifier -81 indicates a physician served as a minimal assistant surgeon for part—not all—of an operative procedure. Use when a physician’s assisting role is limited and does not represent full…- Use CPT modifier -81 when a physician acts as a minimum assistant surgeon who assists during only part (a limited portion) of an operative procedure; append -81 to the primary surgical CPT code to…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207L00000X
    207P00000X
    208000000X
    2084P0800X
    225XM0800X
    207RA0002X
    261QM0801X
    2471R0002X

    Related ICD-10-CM Codes

    24 rows

    ICD-10-CM CodeDescription
    B49Unspecified mycosis
    C71.9Malignant neoplasm of brain, unspecified
    D47.1Chronic myeloproliferative disease
    M00.061Staphylococcal arthritis, right knee
    M00.069Staphylococcal arthritis, unspecified knee
    M00.169Pneumococcal arthritis, unspecified knee
    M05.79Rheumatoid arthritis with rheumatoid factor of multiple sites without organ or systems involvement
    M06.9Rheumatoid arthritis, unspecified
    M08.20Juvenile rheumatoid arthritis with systemic onset, unspecified site
    M10.071Idiopathic gout, right ankle and foot

    Showing 1–10 of 24

    Related Payer Policy Updates

    Specialty: General Surgery1 / 3

    10 rows

    PolicyPayerEffective Date
    Removal of ImplantableBlue Cross Blue Shield - Rhode Island2026-10-01
    Outpatient Surgical Procedures - Site of ServiceUnitedHealthcare2026-09-01
    Surgical ProceduresUnitedHealthcare2026-09-01
    Site of Service Ambulatory Service Center (ASC) Select — Surgical or Diagnostic Procedures in AdultsPremera Bluecross2026-09-01
    Site of Service Ambulatory Surgical Center (ASC) Select — Surgical or Diagnostic Procedures in AdultsPremera Bluecross2026-09-01
    Site of Service Ambulatory Service Center (ASC) SelectPremera Bluecross2026-09-01
    Site of Service Ambulatory Surgical Center (ASC) Select — Site of Service for Ambulatory Surgical Procedures (ASC Select)Premera Bluecross2026-09-01
    Site of Service Ambulatory Service Center (ASC) SelectPremera Bluecross2026-09-01
    Medical Necessity Guidelines Inpatient Surgical CareMass General Brigham Health Plan2026-08-01
    Outpatient Surgical Procedures - Site of Servicesierra health and life2026-08-01

    Related Codes

    CPT-27323-EXCISIONAL-BIOPSY-THIGH-KNEE-SUPERFICIAL-SOFT-TISSUE

    CPT 27323: Excisional Biopsy of Superficial Soft Tissue, Thigh or Knee

    CPT-27324-DEEP-SOFT-TISSUE-BIOPSY-THIGH-KNEE

    CPT 27324: Deep Tissue/Muscle Biopsy of Thigh or Knee

    CPT-27325-HAMSTRING-NEURECTOMY-NERVE-EXCISION

    CPT 27325: Neurectomy of Nerve Along Hamstring

    CPT-27326-POPLITEAL-GASTROCNEMIUS-NERVE-EXCISION-FOR-SPASM

    CPT 27326: Neurectomy of Popliteal/Gastrocnemius Nerve for Spasm

    CPT-27327-SUBCUTANEOUS-TUMOR-EXCISION-THIGH-KNEE

    CPT 27327: Excision of Subcutaneous Tumor of Thigh or Knee (<3 cm)

    CPT-27328-INTRAMUSCULAR-THIGH-KNEE-TUMOR-EXCISION-SMALL

    CPT 27328: Excision of Intramuscular Tumor of Thigh or Knee (<5 cm)

    CPT-27329-EXTENSIVE-EXCISION-SOFT-TISSUE-TUMOR-THIGH-KNEE-LESS-5CM

    CPT 27329: Extensive Excision of Soft-Tissue Tumor, Thigh/Knee (<5 cm)

    CPT-27331-KNEE-ARTHROTOMY-BIOPSY-LOOSE-BODY-REMOVAL

    CPT 27331: Knee Arthrotomy with Synovial Biopsy and Loose Body Removal

    CPT-27332-KNEE-ARTHROTOMY-MENISCAL-EXCISION

    CPT 27332: Arthrotomy with Excision of Medial or Lateral Meniscus

    CPT-27333-KNEE-PARTIAL-MENISCECTOMY-MEDIAL-AND-LATERAL

    CPT 27333: Meniscectomy, Medial and Lateral Knee

    Showing 1–10 of 25

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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