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    v1.0.30
    OpenPayerBilling CodesAutologous Muscle Cell Therapy ProceduresCPT cpt-0999t-autologous-muscle-progenitor-cell-collection
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    CPT 0999T: Muscle Progenitor Cell Collection

    CPT code 0999T describes collection of a patient’s own immature muscle cells (muscle progenitor cells) for future therapeutic use, typically performed as a biologic/tissue harvest procedure; the service involves percutaneous muscle cell collection and the typical site of service is an outpatient surgical suite or ambulatory procedure center where local anesthesia and image guidance (for example, ultrasound) may be used.

    For related coverage guidance, see recent payer policy updates: Orthopedic Joint Procedures of the Knee for Managed Medicaid (MMC), Health and Recovery Plan (HARP), Child Health Plus (CHP), and Essential Plan (EP) Members, Autologous Chondrocyte Transplantation or Implantation Corporate Medical Policy, Autologous Platelet-Derived Growth Factors (Platelet-Rich Plasma [PRP]).

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    Clinical & Coding Specifications

    Clinical Context

    A typical patient is an adult with a focal muscle injury or a progressive neuromuscular condition being evaluated for autologous cell–based therapy. The patient presents to an outpatient procedural suite or ambulatory surgery center for harvesting of immature muscle cells (muscle progenitor cells) to be cryopreserved for potential future use. Pre-procedure evaluation includes medical history, medication reconciliation (anticoagulant management if applicable), informed consent specific to autologous cell collection, and ultrasound review of target muscle anatomy. On the day of service, the patient is positioned so the target muscle is accessible; local anesthesia is administered at the harvest site. The provider uses sterile technique and may employ real-time ultrasound guidance to identify optimal sites within the muscle and to avoid neurovascular structures. A small-volume biopsy or aspiration is obtained, placed into appropriate transport medium, and sent to the processing laboratory for isolation, expansion, and cryopreservation of muscle progenitor cells. Post-procedure monitoring occurs in the recovery area for hemostasis and any immediate complications; the patient receives discharge instructions including wound care and return precautions. Follow-up visits document collection success, any adverse events, and coordination with the processing facility for storage and future therapeutic use.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    0499TProcessing and storage of autologous muscle-derived cells (example: cell isolation, expansion, cryopreservation)Typically performed by the laboratory or vendor following collection under 0999T; documents downstream processing and storage services.
    0589TAutologous cell administration into a target site (example: injection of cultured cells)Represents the therapeutic administration that may follow later after cells collected with 0999T are prepared.
    76942Ultrasonic guidance for needle placement (eg, biopsy, aspiration, injection)Used when real-time ultrasound guidance is employed during muscle progenitor cell collection as noted in 0999T description.
    20550Injection(s) single tendon sheath, or ligament; without ultrasound guidanceMay be paired if a therapeutic injection is performed in the same episode of care, though ultrasound guidance code 76942 is more specific when used.
    99024Postoperative follow-up visit, typically global period servicesUsed to report routine postoperative follow-up related to the collection when payer recognizes this service code.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    25CPT modifier 25 indicates that on the same day as another procedure or service, the same physician or other qualified health care professional provided a significant, separately identifiable…- When to apply: Use modifier 25 only on an E/M code to indicate the same provider delivered a significant, separately identifiable E/M service on the same day as another procedure or service — i.e.,…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    79Modifier 79 indicates a surgical procedure performed during the postoperative global period of a prior surgery that is unrelated to the original procedure. Per AMA/CPT and CMS guidance, it is…- Use modifier 79 when the same physician (or same‑specialty physician in the same group) performs a surgical procedure during the postoperative global period of a prior surgery and the new procedure…
    24Modifier 24 denotes an unrelated evaluation and management (E/M) service performed by the same physician or qualified healthcare professional during a postoperative global period. Per CPT guidance,…- Use modifier 24 when you report an evaluation and management (E/M) service performed by the same physician or same-specialty group during a procedure’s postoperative global period that is unrelated…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RC0000X
    208000000X
    208U00000X
    3336C0002X
    261QP2400X
    246ZB0600X
    246QB0000X
    275N00000X

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    G12.21Amyotrophic lateral sclerosis
    G71.3Mitochondrial myopathy, not elsewhere classified
    M05.79Rheumatoid arthritis with rheumatoid factor of multiple sites without organ or systems involvement
    M06.9Rheumatoid arthritis, unspecified
    M17.11Unilateral primary osteoarthritis, right knee
    M19.071Primary osteoarthritis, right ankle and foot
    M32.9Systemic lupus erythematosus, unspecified
    M51.26Other intervertebral disc displacement, lumbar region
    M51.27Other intervertebral disc displacement, lumbosacral region
    M60.9Myositis, unspecified

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Orthopedics/Musculoskeletal1 / 3

    10 rows

    PolicyPayerEffective Date
    Orthopedic Joint Procedures of the Knee for Managed Medicaid (MMC), Health and Recovery Plan (HARP), Child Health Plus (CHP), and Essential Plan (EP) Membersuniverahealthcare2027-01-01
    Autologous Chondrocyte Transplantation or Implantation Corporate Medical Policybcbsvermont2026-12-01
    Autologous Platelet-Derived Growth Factors (Platelet-Rich Plasma [PRP])Cigna2026-10-15
    Metatarsophalangeal Joint ReplacementCigna2026-10-15
    Shoulder Arthroplasty (Total, Partial, Reverse, Revision and Resurfacing)univerahealthcare2026-10-15
    Hip Arthroplasty (partial and total hip replacement)univerahealthcare2026-10-15
    Artificial Lumbar Intervertebral Disc (Lumbar Total Disc Arthroplasty)univerahealthcare2026-10-15
    Osteochondral Grafting of the Kneeuniverahealthcare2026-10-15
    Metal-on-Metal Total Hip Resurfacinguniverahealthcare2026-10-15
    Lumbar Fusion (arthrodesis) with decompressionuniverahealthcare2026-10-15

    Related Codes

    CPT-1000T-URETHRAL-SPHINCTER-MUSCLE-PROGENITOR-CELL-INJECTION

    CPT 1000T: Urethral Sphincter Muscle Progenitor Cell Injection

    CPT-1001T-AUTOLOGOUS-MUSCLE-PROGENITOR-INJECTION-ANAL-SPHINCTER

    CPT 1001T: Injection of Muscle Progenitor Cells into External Anal Sphincter

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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