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    v1.0.30
    OpenPayerBilling CodesCytogenetic StudiesCPT cpt-88245-chromosome-analysis-sister-chromatid-exchange-sce
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    CPT 88245: Chromosome Analysis for Sister Chromatid Exchange

    CPT code 88245 describes a laboratory cytogenetic test performing chromosome analysis for Sister Chromatid Exchange (SCE) on cultured lymphocyte cells from blood or bone marrow, evaluating approximately 20–25 cells; service type: cytogenetic chromosome analysis for SCE testing; typical site of service: clinical cytogenetics laboratory or hospital laboratory performing cultured cell analysis.

    For related coverage guidance, see recent payer policy updates: Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency, Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policy.

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    Clinical & Coding Specifications

    Clinical Context

    A 32-year-old patient referred from hematology presents with unexplained chromosomal instability and suspected chromosomal breakage disorder after recurrent unexplained cytopenias and abnormal peripheral blood smear. The clinician orders a Sister Chromatid Exchange (SCE) analysis to evaluate chromosomal breakage characteristics using cultured lymphocytes from peripheral blood. Blood is collected in appropriate anticoagulant and sent to the cytogenetics laboratory. In the lab, a technologist cultures lymphocytes, incorporates bromodeoxyuridine (BrdU) to label sister chromatids, harvests metaphase cells, and prepares slides. A cytogeneticist or qualified laboratory analyst examines 20–25 metaphase cells microscopically to count SCEs per chromosome and documents increased exchange frequency when present. Results are reported to the ordering hematologist or genetics specialist for correlation with clinical findings and potential further genetic evaluation.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    88240Chromosome analysis, bone marrow, blood, or lymph node; culture and harvestOften used for standard karyotyping in parallel with SCE when broader chromosomal analysis is needed
    88244Chromosome analysis, blood or bone marrow; enhanced study requiring specialized techniquesMay be performed when additional cytogenetic techniques are required beyond routine karyotype and SCE
    88247Chromosome analysis, blood or bone marrow; FISH probe study (single probe)Fluorescence in situ hybridization may be used adjunctively to detect specific chromosomal abnormalities suggested by SCE findings
    88342Immunohistochemistry, per single antigen; initial single antibody stainMay be performed on concurrent specimens in hematologic workups that accompany SCE testing
    81229BRCA1 analysis, full sequence analysis (example of molecular testing)Molecular genetic testing may follow abnormal SCE or cytogenetic findings to identify gene-level defects

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207Q00000X
    208000000X
    207RC0000X
    207L00000X
    163WP1700X
    2083C0008X
    101YM0800X
    163WN0003X

    Related ICD-10-CM Codes

    24 rows

    ICD-10-CM CodeDescription
    C92.90Myeloid leukemia, unspecified, not having achieved remission
    C93.10Chronic myelomonocytic leukemia not having achieved remission
    C95.90Leukemia, unspecified not having achieved remission
    D46.9Myelodysplastic syndrome, unspecified
    D47.9Neoplasm of uncertain behavior of lymphoid, hematopoietic and related tissue, unspecified
    D59.9Acquired hemolytic anemia, unspecified
    D61.9Aplastic anemia, unspecified
    D70.9Neutropenia, unspecified
    E03.9Hypothyroidism, unspecified
    E34.9Endocrine disorder, unspecified

    Showing 1–10 of 24

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Carrier Testing Panels for Genetic Diseases (for Kentucky Only)UnitedHealthcare2026-10-01
    Genetic Testing for Neurological Disorders (for Kansas Only)UnitedHealthcare2026-10-01
    Genetic and Molecular TestingProvidence Health Plan2026-10-01
    Cell-Free Fetal DNA Testing (for Idaho Only)UnitedHealthcare2026-10-01
    Next Generation Sequencing for Minimal Residual Disease DetectionProvidence Health Plan2026-10-01
    Pharmacogenetic Panel Testing (for Kansas Only)UnitedHealthcare2026-10-01
    Non-Covered Genetic Panel TestsProvidence Health Plan2026-10-01

    Related Codes

    CPT-88230-LYMPHOCYTE-TISSUE-CULTURE-NONNEOPLASTIC-DISORDER

    CPT 88230: Lymphocyte Culture for Nonneoplastic Disorder

    CPT-88233-TISSUE-CULTURE-SKIN-SOLID-TISSUE-NONNEOPLASTIC

    CPT 88233: Tissue Culture for Nonneoplastic Disorders

    CPT-88235-AMNIOTIC-FLUID-CHORIONIC-VILLUS-TISSUE-CULTURE

    CPT 88235: Tissue Culture Preparation from Amniotic Fluid or Chorionic Villi

    CPT-88237-BONE-MARROW-BLOOD-CELL-CULTURE-NEOPLASTIC-DISORDER

    CPT 88237: Cell Culture for Neoplastic Disorders

    CPT-88239-SOLID-TUMOR-CELL-CULTURE-NEOPLASTIC-DISORDER

    CPT 88239: Cell Culture From Solid Tumor for Neoplastic Disorder

    CPT-88240-CELL-LINE-CRYOPRESERVATION-STORAGE

    CPT 88240: Cryopreservation and Storage of Cell Line

    CPT-88241-THAW-AND-EXPAND-FROZEN-CELLS-SINGLE-ALIQUOT

    CPT 88241: Thaw and Expand Previously Frozen Cells, Single Aliquot

    CPT-88248-CHROMOSOME-ANALYSIS-BREAKAGE-SYNDROME

    CPT 88248: Chromosome Analysis for Breakage Syndromes

    CPT-88249-CHROMOSOME-ANALYSIS-BREAKAGE-SYNDROME-CLASTOGEN-STRESS

    CPT 88249: Chromosome Analysis for Breakage Syndrome, 100-Cell Clastogen Test

    CPT-88261-CHROMOSOME-ANALYSIS-KARYOTYPE-BANDING

    CPT 88261: Chromosome Analysis, Karyotype with Banding

    Showing 1–10 of 26

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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