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    v1.0.28
    OpenPayerBilling CodesTransfusion Medicine ProceduresCPT cpt-86921-blood-crossmatch-incubation-compatibility-test
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    CPT 86921: Antiglobulin Crossmatch, Incubation Technique

    CPT code 86921 describes an antiglobulin crossmatch performed by a laboratory analyst to assess compatibility between a donor blood unit and a patient’s serum using an incubation technique; this service is a blood bank transfusion compatibility test typically performed in a clinical laboratory or hospital blood bank setting and is billed as a laboratory procedure for pretransfusion testing.

    For related coverage guidance, see recent payer policy updates: Epithelial Cell Cytology in Breast Cancer Risk Assessment, Chemotherapy Observation or Inpatient Hospitalization (for New Mexico Only), Antibody-Drug Conjugates.

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    Clinical & Coding Specifications

    Clinical Context

    A hospitalized adult patient requires transfusion of packed red blood cells for symptomatic anemia following major surgery. The patient has a recent transfusion history and a positive antibody screen. A blood bank technologist performs a compatibility test using an incubation technique to determine if a selected donor unit is compatible with the patient's serum; this is documented as a crossmatch. Specimen collection occurs in the inpatient ward or preoperative area, the sample and donor unit are processed in the hospital blood bank or transfusion laboratory, and results are reported to the ordering clinician to authorize transfusion. Typical workflow: order placed by physician or transfusion service → patient specimen labeled and sent to transfusion lab → antibody screen and identification if needed → 86921 incubation crossmatch performed against selected donor unit → results documented and communicated; compatible units are issued and transported to the floor for administration.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86900Compatibility test, immediate spin crossmatch for alloantibody-negative patientsOften performed prior to or instead of an incubation crossmatch when no clinically significant antibodies are detected; simpler, faster compatibility assessment.
    86901Blood compatibility test; major crossmatch with automation or electronic crossmatch proceduresRelated as alternative methods of compatibility testing when automation or electronic crossmatch is appropriate.
    86850Antibody identification, including titer and specificity when indicatedPerformed when the antibody screen is positive and identification is required before performing 86921 to select compatible units.
    86909Antihuman globulin crossmatch (AHG), incompatibility testingAHG techniques are part of compatibility testing strategy and may be performed in conjunction with or as part of incubation methods.
    87880Infectious agent detection by immunoassay, qualitative or semiquantitative; Hepatitis B surface antigenPerformed previously for donor unit testing in blood center workflows; complements compatibility testing by ensuring donor safety.

    Related Modifiers

    7 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207ZP0105XClinical Pathology/Laboratory Medicine PhysicianA pathologist deals with the causes and nature of disease and contributes to diagnosis, prognosis and treatment through knowledge gained by the labora…
    207RC0000XCardiovascular Disease PhysicianAn internist who specializes in diseases of the heart and blood vessels and manages complex cardiac conditions such as heart attacks and life-threaten…
    207RH0002XHospice and Palliative Medicine (Internal Medicine) PhysicianAn internal medicine physician with special knowledge and skills to prevent and relieve the suffering experienced by patients with life-limiting illne…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    172A00000XDriverA person employed to operate a motor vehicle as a carrier of persons or property.
    2278G1100XGeneral Care Certified Respiratory TherapistThis level of care includes diagnostics testing, therapeutics, monitoring, rehabilitation of patients with disorders of the cardiopulmonary system, as…
    163WN0002XNeonatal Intensive Care Registered NurseNursing Service Providers · Registered Nurse
    363LN0005XCritical Care Neonatal Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner

    Related ICD-10-CM Codes

    28 rows

    ICD-10-CM CodeDescription
    A41.9Sepsis, unspecified organism
    C34.90Malignant neoplasm of unspecified part of unspecified bronchus or lung
    C50.912Malignant neoplasm of unspecified site of left female breast
    C82.90Follicular lymphoma, unspecified, unspecified site
    C91.10Chronic lymphocytic leukemia of B-cell type not having achieved remission
    D50.9Iron deficiency anemia, unspecified
    D62Acute posthemorrhagic anemia
    D64.9Anemia, unspecified
    D66Hereditary factor VIII deficiency
    D67Hereditary factor IX deficiency

    Showing 1–10 of 28

    Related Payer Policy Updates

    Specialty: Hematology/Oncology1 / 3

    4 rows

    PolicyPayerEffective Date
    Epithelial Cell Cytology in Breast Cancer Risk AssessmentOscar Health2026-10-01
    Chemotherapy Observation or Inpatient Hospitalization (for New Mexico Only)UnitedHealthcare2026-08-01
    Antibody-Drug ConjugatesPremera Bluecross2026-08-01
    Umbilical Cord Blood Harvesting and Storage for Future Use (for Indiana Only)UnitedHealthcare2026-08-01

    Related Codes

    CPT-86850-RBC-ANTIBODY-SCREENING

    CPT 86850: Screening Test for Non‑A/B Red Blood Cell Antibodies

    CPT-86860-RBC-ELUTION-IMMUNOHEMATOLOGY-BLOOD-BANK

    CPT 86860: RBC Elution for Antibody Detection

    CPT-86870-RBC-ANTIBODY-IDENTIFICATION-IMMUNOHEMATOLOGY

    CPT 86870: Antibody Identification to Red Blood Cells

    CPT-86880-DIRECT-ANTIGLOBULIN-TEST-DAT-COOMBS

    CPT 86880: Direct Antihuman Globulin (Coombs) Test

    CPT-86885-INDIRECT-ANTIGLOBULIN-COOMBS-TEST

    CPT 86885: Indirect Antiglobulin (Coombs) Test for Serum Antibodies

    CPT-86886-INDIRECT-ANTIGLOBULIN-COOMBS-TEST-SERIAL-DILUTIONS

    CPT 86886: Indirect Antihuman Globulin (Coombs) Test

    CPT-86890-AUTOLOGOUS-BLOOD-COLLECTION-PROCESSING-STORAGE

    CPT 86890: Autologous Blood Collection, Processing, and Storage

    CPT-86891-INTRAOPERATIVE-BLOOD-SALVAGE-AUTOTRANSFUSION

    CPT 86891: Intraoperative Blood Salvage (Cell Salvage)

    CPT-86900-ABO-BLOOD-TYPING-SERUM-PLASMA

    CPT 86900: ABO Blood Typing Using Serum or Plasma

    CPT-86901-RH-BLOOD-GROUP-TYPING-SEROLOGIC-PLASMA-SERUM

    CPT 86901: Rh Blood Group Serologic Typing, Serum/Plasma

    Showing 1–10 of 38

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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