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    v1.0.28
    OpenPayerBilling CodesQualitative or Semiquantitative ImmunoassaysCPT cpt-86664-ebv-ebna-antibody-immunoassay
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    CPT 86664: EBV EBNA Antibody Immunoassay

    CPT code 86664 reports an immunoassay performed by a laboratory analyst to detect antibodies to Epstein–Barr virus nuclear antigen (EBNA) in patient serum; this is a serologic antibody test used to assess prior EBV infection or immune status and is typically performed as a laboratory serology test in a clinical laboratory or hospital laboratory setting.

    For related coverage guidance, see recent payer policy updates: Diagnosis of Idiopathic Environmental Intolerance, Allergen Testing, Allergen Testing.

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    Clinical & Coding Specifications

    Clinical Context

    A young adult presents to an outpatient clinic with fever, sore throat, cervical lymphadenopathy, fatigue, and atypical lymphocytosis on a complete blood count. The primary care clinician orders serologic testing for Epstein–Barr virus (EBV) to distinguish acute infection from past exposure. A venous blood specimen is collected at the clinic or a phlebotomy draw station and sent to the hospital or reference laboratory. The laboratory performs an immunoassay targeting Epstein–Barr nuclear antigen (EBNA) IgG antibodies using automated analyzers. Results are reported to the ordering clinician; a positive EBNA IgG with negative acute markers suggests past infection, while absence of EBNA with positive viral capsid antigen (VCA) IgM suggests recent or acute EBV infection. Typical sites of service include outpatient clinics, hospital outpatient laboratories, community phlebotomy centers, and independent reference laboratories. Billing reflects a laboratory immunoassay service performed by clinical laboratory personnel under CPT code 86664.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    86664Antibody; Epstein-Barr virus nuclear antigen (EBNA) (e.g., IgG)Primary code for the EBNA IgG immunoassay described.
    86663Antibody; Epstein-Barr virus viral capsid antigen (VCA), IgMCommonly ordered alongside 86664 to identify acute EBV infection (VCA IgM positive with EBNA negative indicates recent infection).
    86665Antibody; Epstein-Barr virus viral capsid antigen (VCA), IgGUsed with 86664 to determine immune status versus past infection (VCA IgG and EBNA IgG positive consistent with past infection).
    86669Antibody; mononucleosis (Epstein-Barr) heterophile test (e.g., Monospot)Rapid screening test often used initially in outpatient settings; positive Monospot may prompt confirmatory testing with 86663/86664.
    80053Comprehensive metabolic panelFrequently ordered in same encounter to assess hepatic function in suspected infectious mononucleosis.

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    95Modifier 95 indicates that a service was provided via synchronous, real‑time audio‑video telehealth rather than in‑person. Per CMS, AAPC and professional associations, append modifier 95 to claim…- Use modifier 95 to indicate a service was furnished via synchronous, real‑time audiovisual telehealth (a live audio‑video conference between patient and provider) rather than in‑person. - Pair…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207Q00000XFamily Medicine PhysicianFamily Medicine is the medical specialty which is concerned with the total health care of the individual and the family. It is the specialty in breadt…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    208D00000XGeneral Practice PhysicianA physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
    207R00000XInternal Medicine PhysicianA physician who provides long-term, comprehensive care in the office and the hospital, managing both common and complex illness of adolescents, adults…
    208000000XPediatrics PhysicianA pediatrician is concerned with the physical, emotional and social health of children from birth to young adulthood. Care encompasses a broad spectru…
    163WR0400XRehabilitation Registered NurseNursing Service Providers · Registered Nurse
    2279E0002XEmergency Care Registered Respiratory TherapistThe immediate availability of diagnostic and therapeutic cardiopulmonary services in the assessment and management of trauma victims, patients requiri…
    363LG0600XGerontology Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    A54.02Gonococcal vulvovaginitis, unspecified
    B27.90Infectious mononucleosis, unspecified without complication
    B34.9Viral infection, unspecified
    B95.8Unspecified staphylococcus as the cause of diseases classified elsewhere
    D69.6Thrombocytopenia, unspecified
    H66.90Otitis media, unspecified, unspecified ear
    H92.09Otalgia, unspecified ear
    J02.9Acute pharyngitis, unspecified
    J06.9Acute upper respiratory infection, unspecified
    L29.9Pruritus, unspecified

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Allergy/Clinical Immunology1 / 3

    9 rows

    PolicyPayerEffective Date
    Diagnosis of Idiopathic Environmental IntoleranceOscar Health2026-10-01
    Allergen Testingblue cross blue shield - louisiana2026-09-15
    Allergen TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Hereditary Angioedema - EkterlyCigna2026-08-15
    Omalizumab products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab Products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab ProductsPremera Bluecross2026-08-01
    Allergy Testinguniverahealthcare2026-07-16
    Omalizumab (Xolair) and related biologics coverageMass General Brigham Health Plan2026-07-01

    Related Codes

    86803-QUALITATIVE-OR-SEMIQUANTITATIVE-IMMUNOASSAYS-HEPATITIS-C

    CPT 86803: Hepatitis C Antibody Immunoassay in Serum

    CPT-86000-FEBRILE-AGGLUTININS-SEROLOGIC-TEST

    CPT 86000: Febrile Agglutinins (Serologic Agglutination) Test

    CPT-86001-ALLERGEN-SPECIFIC-IGG-LABORATORY-TEST

    CPT 86001: Allergen-Specific IgG Assay, Technical Component

    CPT-86003-ALLERGEN-SPECIFIC-IGE-ASSAY-TECHNICAL

    CPT 86003: Allergen‑Specific IgE Assay Using Crude Allergen Extract

    CPT-86005-MULTI-ALLERGEN-IGE-SCREENING-QUALITATIVE

    CPT 86005: Multi-Allergen Allergen-Specific IgE Screening

    CPT-86008-ALLERGEN-COMPONENT-SPECIFIC-IGE-ASSAY

    CPT 86008: Allergen Component–Specific IgE Assay

    CPT-86015-ANTI-ACTIN-SMOOTH-MUSCLE-ANTIBODY-ASMA

    CPT 86015: Actin (Smooth Muscle) Antibody (ASMA) Immunoassay

    CPT-86021-LEUKOCYTE-ANTIBODY-IDENTIFICATION-LAB-TECHNICAL

    CPT 86021: Leukocyte Antibody Identification Laboratory Test

    CPT-86022-PLATELET-ANTIBODY-IDENTIFICATION-TECHNICAL-TEST

    CPT 86022: Platelet Antibody Detection and Identification

    CPT-86023-PLATELET-ASSOCIATED-IMMUNOGLOBULIN-DETECTION

    CPT 86023: Platelet-Associated Immunoglobulin Assay

    Showing 1–10 of 199

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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