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    v1.0.28
    OpenPayerBilling CodesQualitative or Semiquantitative ImmunoassaysCPT cpt-86334-immunofixation-electrophoresis-serum
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    CPT 86334: Immunofixation Electrophoresis, Serum

    Medicare pays $18 on average nationally for this procedure.

    CPT code 86334 reports immunofixation electrophoresis performed on serum to identify and characterize monoclonal immunoglobulins for diagnosis or monitoring of monoclonal gammopathies and multiple myeloma; this is a laboratory diagnostic test typically provided by clinical laboratories or hospital lab services with the typical site of service being an outpatient laboratory or hospital laboratory where serum specimens are processed and analyzed.

    For related coverage guidance, see recent payer policy updates: Diagnosis of Idiopathic Environmental Intolerance, Allergen Testing, Allergen Testing.

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    Clinical & Coding Specifications

    Clinical Context

    A 62-year-old outpatient with progressive anemia, renal insufficiency, hypercalcemia, and unexplained bone pain is referred to hematology for evaluation of a suspected monoclonal gammopathy. The clinician orders serum protein electrophoresis followed by immunofixation electrophoresis to characterize and confirm a monoclonal (M) protein. A phlebotomist draws a serum sample in the ambulatory clinic; the specimen is transported to the clinical laboratory. A medical technologist performs immunofixation electrophoresis (86334) on the serum to identify immunoglobulin heavy- and light-chain isotypes (for example, IgG kappa). Results are reported to the ordering hematologist for diagnostic correlation, staging, and treatment planning. Typical sites of service include hospital outpatient laboratories, independent reference laboratories, and ambulatory clinic laboratories when specimen processing is performed onsite. The workflow commonly follows: order entry → specimen collection → specimen accessioning → electrophoresis and immunofixation assay → interpretive review by a qualified laboratory physician or pathologist → result reporting to the ordering provider.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80053Comprehensive metabolic panelProvides renal function and calcium results often used in the workup and monitoring of multiple myeloma and related disorders; commonly ordered at the same encounter.
    80061Lipid panelOften part of general baseline labs; less directly related but commonly ordered during comprehensive evaluations.
    84166Free light chains, quantitativeMeasures serum free kappa and lambda light chains; frequently used alongside immunofixation to detect and monitor light-chain disease.
    86336Protein electrophoresis, quantitative; serumOften performed prior to or with immunofixation to detect and quantify M-protein bands that immunofixation then characterizes.
    88175Flow cytometry, cell surface markers, per specimenUsed on bone marrow or peripheral blood to characterize clonal plasma cell populations when malignancy is suspected; complements immunofixation findings.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…
    95Modifier 95 indicates that a service was provided via synchronous, real‑time audio‑video telehealth rather than in‑person. Per CMS, AAPC and professional associations, append modifier 95 to claim…- Use modifier 95 to indicate a service was furnished via synchronous, real‑time audiovisual telehealth (a live audio‑video conference between patient and provider) rather than in‑person. - Pair…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207RC0000XCardiovascular Disease PhysicianAn internist who specializes in diseases of the heart and blood vessels and manages complex cardiac conditions such as heart attacks and life-threaten…
    2080P0206XPediatric Gastroenterology PhysicianA pediatrician who specializes in the diagnosis and treatment of diseases of the digestive systems of infants, children and adolescents. This speciali…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    207LP2900XPain Medicine (Anesthesiology) PhysicianAn anesthesiologist who provides a high level of care, either as a primary physician or consultant, for patients experiencing problems with acute, chr…
    207RN0300XNephrology PhysicianAn internist who treats disorders of the kidney, high blood pressure, fluid and mineral balance and dialysis of body wastes when the kidneys do not fu…
    251G00000XCommunity Based Hospice Care AgencyAgencies · Hospice Care, Community Based
    231HA2400XAssistive Technology Practitioner AudiologistSpeech, Language and Hearing Service Providers · Audiologist
    320600000XIntellectual and/or Developmental Disabilities Residential Treatment FacilityA residential facility that provides habilitation services and other care and treatment to adults or children diagnosed with developmental and intelle…

    Related ICD-10-CM Codes

    25 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    C90.00Multiple myeloma not having achieved remission
    C90.01Multiple myeloma in remission
    C90.02Multiple myeloma in relapse
    C90.10Plasma cell leukemia not having achieved remission
    D47.2Monoclonal gammopathy
    D61.9Aplastic anemia, unspecified
    D63.0Anemia in neoplastic disease
    D89.9Disorder involving the immune mechanism, unspecified
    E83.50Unspecified disorder of calcium metabolism

    Showing 1–10 of 25

    Related Payer Policy Updates

    Specialty: Allergy/Clinical Immunology1 / 3

    10 rows

    PolicyPayerEffective Date
    Diagnosis of Idiopathic Environmental IntoleranceOscar Health2026-10-01
    Allergen Testingblue cross blue shield - louisiana2026-09-15
    Allergen TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Hereditary Angioedema - EkterlyCigna2026-08-15
    Omalizumab products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab Products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab ProductsPremera Bluecross2026-08-01
    Allergy Testinguniverahealthcare2026-07-16
    Omalizumab (Xolair) and related biologics coverageMass General Brigham Health Plan2026-07-01
    Xolair (omalizumab) (Subcutaneous) — Concomitant therapy restrictions and dosing guidanceeocco2026-07-01

    Related Codes

    86803-QUALITATIVE-OR-SEMIQUANTITATIVE-IMMUNOASSAYS-HEPATITIS-C

    CPT 86803: Hepatitis C Antibody Immunoassay in Serum

    CPT-86000-FEBRILE-AGGLUTININS-SEROLOGIC-TEST

    CPT 86000: Febrile Agglutinins (Serologic Agglutination) Test

    CPT-86001-ALLERGEN-SPECIFIC-IGG-LABORATORY-TEST

    CPT 86001: Allergen-Specific IgG Assay, Technical Component

    CPT-86003-ALLERGEN-SPECIFIC-IGE-ASSAY-TECHNICAL

    CPT 86003: Allergen‑Specific IgE Assay Using Crude Allergen Extract

    CPT-86005-MULTI-ALLERGEN-IGE-SCREENING-QUALITATIVE

    CPT 86005: Multi-Allergen Allergen-Specific IgE Screening

    CPT-86008-ALLERGEN-COMPONENT-SPECIFIC-IGE-ASSAY

    CPT 86008: Allergen Component–Specific IgE Assay

    CPT-86015-ANTI-ACTIN-SMOOTH-MUSCLE-ANTIBODY-ASMA

    CPT 86015: Actin (Smooth Muscle) Antibody (ASMA) Immunoassay

    CPT-86021-LEUKOCYTE-ANTIBODY-IDENTIFICATION-LAB-TECHNICAL

    CPT 86021: Leukocyte Antibody Identification Laboratory Test

    CPT-86022-PLATELET-ANTIBODY-IDENTIFICATION-TECHNICAL-TEST

    CPT 86022: Platelet Antibody Detection and Identification

    CPT-86023-PLATELET-ASSOCIATED-IMMUNOGLOBULIN-DETECTION

    CPT 86023: Platelet-Associated Immunoglobulin Assay

    Showing 1–10 of 199

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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