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    OpenPayerBilling CodesHematology and Coagulation ProceduresCPT cpt-85335-coagulation-factor-inhibitor-assessment-plasma
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    CPT 85335: Plasma Analysis for Coagulation Factor Inhibitor Assessment

    CPT code 85335 describes a laboratory procedure in which a lab analyst evaluates patient plasma to assess the results of a positive coagulation factor inhibitor screen; this is a specialized coagulation/hematology test performed in a clinical laboratory setting, typically as a follow-up diagnostic assay after screening for inhibitor activity.

    For related coverage guidance, see recent payer policy updates: Bendamustine Products Coverage Criteria, T-Cell Lymphoma Agents: Beleodaq, Istodax, Lymphir, Poteligeo, and Romidepsin, Brentuximab Vedotin (Adcetris®) Coverage Criteria.

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    Clinical & Coding Specifications

    Clinical Context

    A 52-year-old inpatient presents with spontaneous bruising and prolonged bleeding after a minor dental procedure. Initial coagulation studies demonstrate an isolated prolonged activated partial thromboplastin time (aPTT) that does not fully correct on a mixing study, suggesting a possible coagulation factor inhibitor. A plasma specimen is sent to the hospital clinical laboratory for specialized testing. The laboratory analyst performs an assay using patient plasma to assess the results of a positive coagulation factor inhibitor screen (neutralization or Bethesda-type assay) to quantify inhibitor titer and identify the specific factor affected (commonly factor VIII).

    Typical clinical workflow:

    • Clinician orders coagulation studies for bleeding evaluation, which include aPTT and prothrombin time (PT).

    • If aPTT is prolonged and a mixing study suggests an inhibitor, the laboratory performs confirmatory testing, including factor activity assays and an inhibitor assay represented by 85335.

    • The laboratory analyst prepares patient plasma, performs the inhibitor assay (e.g., Bethesda or Nijmegen-modified Bethesda), records inhibitor titer, and reports results to the ordering clinician.

    • Results guide hematology consultation and management decisions such as immunosuppression, bypassing agents, or factor replacement.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    85335Assay for coagulation factor inhibitor; patient plasma assay to assess results of a positive inhibitor screenPrimary procedure described; quantifies inhibitor titer and confirms presence of a factor-specific inhibitor.
    85730Coagulation time measurement, reported as prothrombin time (PT) and/or international normalized ratio (INR)Often ordered earlier in the bleeding workup alongside 85335 to evaluate extrinsic pathway.
    85731Activated partial thromboplastin time (aPTT)Initial screening test that frequently leads to performance of 85335 when mixing studies suggest an inhibitor.
    85376Coagulation factor activity assay, multiple factors, performed by one-stage clotting methods (each factor reported separately)Performed to measure specific factor activity (e.g., factor VIII) when inhibitor is suspected; results guide interpretation of 85335.
    85342Mixing study interpretation (therapeutic drug monitoring/coagulation)Mixing studies are used before performing inhibitor assays; represents related laboratory analyses in the diagnostic sequence.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RC0000X
    207L00000X
    213E00000X
    2251X0800X
    163WP0809X
    207QA0000X
    146M00000X
    125J00000X

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    D65Disseminated intravascular coagulation [defibrination syndrome]
    D68.4Acquired coagulation factor deficiency
    D68.8Other specified coagulation defects
    D68.9Coagulation defect, unspecified
    D69.0Allergic purpura
    D69.3Immune thrombocytopenic purpura
    D69.6Thrombocytopenia, unspecified
    I48.91Unspecified atrial fibrillation
    I50.9Heart failure, unspecified
    N17.9Acute kidney failure, unspecified

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Hematology/Oncology1 / 3

    10 rows

    PolicyPayerEffective Date
    Bendamustine Products Coverage Criteriablue cross blue shield - tennessee2027-01-30
    T-Cell Lymphoma Agents: Beleodaq, Istodax, Lymphir, Poteligeo, and RomidepsinMass General Brigham Health Plan2027-01-04
    Brentuximab Vedotin (Adcetris®) Coverage Criteriablue cross blue shield - tennessee2026-12-31
    Pemetrexed Coverageblue cross blue shield - tennessee2026-12-31
    Tumor Treatment Fields - Medical PolicyHealth-New-England2026-12-01
    Extracorporeal PhotopheresisCigna2026-11-15
    VonvendiCigna2026-11-15
    Hematology - AphexdaCigna2026-11-15
    Extracorporeal PhotopheresisCigna2026-11-15
    Yartemlea (narsoplimab-wuug) Coverage for TA-TMANeighborhood Health Plan of Rhode Island2026-11-01

    Related Codes

    CPT-85002-BLEEDING-TIME-TEST-LABORATORY

    CPT 85002: Bleeding Time Test, Technical Component

    CPT-85004-AUTOMATED-DIFFERENTIAL-WHITE-BLOOD-CELL-COUNT

    CPT 85004: Automated White Blood Cell Differential

    CPT-85007-MANUAL-DIFFERENTIAL-WHITE-BLOOD-CELL-COUNT

    CPT 85007: Manual Differential White Blood Cell Count

    CPT-85008-BLOOD-SMEAR-MORPHOLOGIC-REVIEW-NO-DIFFERENTIAL

    CPT 85008: Microscopic Blood Smear Examination, No Differential

    CPT-85009-MANUAL-WBC-COUNT-BUFFY-COAT

    CPT 85009: Manual White Blood Cell Count Using Buffy Coat

    CPT-85013-MICROHEMATOCRIT-TEST-LABORATORY-DIAGNOSTIC

    CPT 85013: Microhematocrit Test by Tube Centrifugation

    CPT-85014-HEMATOCRIT-VOLUME-PERCENT-RED-BLOOD-CELLS

    CPT 85014: Hematocrit (Volume Percent of Red Blood Cells)

    CPT-85018-HEMOGLOBIN-ASSAY-WHOLE-BLOOD

    CPT 85018: Hemoglobin Measurement in Whole Blood

    CPT-85025-COMPLETE-BLOOD-COUNT-CBC

    CPT 85025: Complete Blood Count (CBC)

    CPT-85027-COMPLETE-BLOOD-COUNT-HGB-HCT

    CPT 85027: Complete Blood Count with Hemoglobin and Hematocrit

    Showing 1–10 of 99

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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    85342