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    v1.0.28
    OpenPayerBilling CodesChemistry ProceduresCPT cpt-84165-serum-protein-electrophoresis-spep
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    CPT 84165: Serum Protein Electrophoresis (SPEP)

    Medicare pays $18 on average nationally for this procedure.

    CPT code 84165 describes the laboratory technical procedure for serum protein electrophoresis (SPE or SPEP), a test in which a lab analyst separates and quantifies serum protein fractions to generate an electrophoretic pattern used in diagnosing plasma cell dyscrasias, liver and kidney disease, and immune disorders; service type: clinical laboratory test (serum protein fractionation); typical site of service: clinical laboratory or hospital outpatient laboratory.

    For related coverage guidance, see recent payer policy updates: Salivary Hormone Testing, Parathyroid Hormone, Phosphorus, Calcium, and Magnesium Testing, Bone Turnover Markers Testing.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 62-year-old outpatient with progressive fatigue, unexplained weight loss, and an elevated total protein on basic metabolic panel is referred for serum protein electrophoresis. The clinician orders 84165 to fractionate and quantitate serum protein components to detect monoclonal gammopathy, evaluate suspected multiple myeloma, assess chronic liver disease with abnormal albumin/globulin ratio, or investigate renal protein loss. The typical workflow: the provider places an order for 84165 in the electronic health record, the patient presents to the outpatient phlebotomy or hospital laboratory, a venous blood sample is collected and processed, the laboratory analyst performs serum protein electrophoresis (SPEP) using electrophoretic separation and densitometry to quantitate fractions (albumin, alpha, beta, gamma), the lab issues a report with fraction percentages and M‑protein characterization, and the ordering clinician interprets results in the context of clinical findings and may order reflex testing (immunofixation, quantitative immunoglobulins) as indicated.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    85662Immunoelectrophoresis, serumOften used after 84165 when an abnormal SPEP requires further characterization of immunoglobulin classes and presence of monoclonal proteins.
    86353Immunoglobulin, quantitative; IgGUsed alongside SPEP to quantify specific immunoglobulin classes when evaluating hypogammaglobulinemia or monoclonal gammopathy.
    82784Protein; total (total protein)Frequently ordered prior to or with SPEP as part of initial protein assessment and can prompt SPEP when abnormal.
    84156Albumin; serumProvides albumin concentration used with SPEP fractionation to calculate absolute values for albumin and globulin fractions.
    83303Therapeutic drug assay, immunoassay, quantitative; trough, miscellaneousIn contexts where monoclonal antibody therapies or other biologics are monitored, quantitative assays may be ordered in related workflows.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207R00000XInternal Medicine PhysicianA physician who provides long-term, comprehensive care in the office and the hospital, managing both common and complex illness of adolescents, adults…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    207RG0300XGeriatric Medicine (Internal Medicine) PhysicianAn internist who has special knowledge of the aging process and special skills in the diagnostic, therapeutic, preventive and rehabilitative aspects o…
    207LP2900XPain Medicine (Anesthesiology) PhysicianAn anesthesiologist who provides a high level of care, either as a primary physician or consultant, for patients experiencing problems with acute, chr…
    281PC2000XChildren's Chronic Disease HospitalHospitals · Chronic Disease Hospital
    2865X1600XOperational (Transportable) Military General Acute Care HospitalA Department of Defense (DoD) health care organization furnishing inpatient care 24 hours per day in "non-fixed" or deployed facilities. Entity is not…
    101YP2500XProfessional CounselorBehavioral Health & Social Service Providers · Counselor

    Related ICD-10-CM Codes

    26 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    C90.00Multiple myeloma not having achieved remission
    C90.02Multiple myeloma in relapse
    D47.2Monoclonal gammopathy
    D50.9Iron deficiency anemia, unspecified
    D63.8Anemia in other chronic diseases classified elsewhere
    D89.9Disorder involving the immune mechanism, unspecified
    E10.9Type 1 diabetes mellitus without complications
    E11.9Type 2 diabetes mellitus without complications
    E78.5Hyperlipidemia, unspecified

    Showing 1–10 of 26

    Related Payer Policy Updates

    Specialty: Endocrinology1 / 3

    10 rows

    PolicyPayerEffective Date
    Salivary Hormone TestingOscar Health2026-10-01
    Parathyroid Hormone, Phosphorus, Calcium, and Magnesium TestingOscar Health2026-10-01
    Bone Turnover Markers TestingOscar Health2026-10-01
    Testosterone — Coverage Criteria for Measurement and MonitoringOscar Health2026-10-01
    Diabetes Mellitus Testing — Coverage CriteriaOscar Health2026-10-01
    Thyroid Disease TestingOscar Health2026-10-01
    Thyroid Disease Testingblue cross blue shield - louisiana2026-09-15
    Thyroid Disease TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Parathyroid Hormone, Phosphorous, Calcium and Magnesium TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Testosterone laboratory testing and managementPremera Bluecross2026-09-04

    Related Codes

    CPT-82009-KETONE-BODIES-QUALITATIVE-TEST

    CPT 82009: Qualitative Ketone Bodies Test

    CPT-82010-QUANTITATIVE-KETONE-BODIES-ASSAY-SERUM-PLASMA

    CPT 82010: Quantitative Ketone Bodies in Serum or Plasma

    CPT-82013-ACETYLCHOLINESTERASE-QUANTITATIVE-ASSAY

    CPT 82013: Quantitative Acetylcholinesterase (Acetylhydrolase) Assay

    CPT-82016-QUALITATIVE-ACYLCARNITINES-METABOLIC-SCREEN

    CPT 82016: Qualitative Acylcarnitines, Plasma/Serum/Urine

    CPT-82017-QUANTITATIVE-ACYLCARNITINE-ANALYSIS

    CPT 82017: Quantitative Acylcarnitine Analysis

    CPT-82024-QUANTITATIVE-CORTICOTROPIN-ACTH-ASSAY

    CPT 82024: Adrenocorticotropic Hormone (ACTH) Quantitative Assay

    CPT-82030-QUANTITATIVE-CAMP-PLASMA-URINE-ASSAY

    CPT 82030: Quantitative cyclic AMP assay on plasma or urine

    CPT-82040-QUANTITATIVE-ALBUMIN-MEASUREMENT

    CPT 82040: Quantitative Albumin Measurement

    CPT-82042-QUANTITATIVE-ALBUMIN-NONBLOOD-FLUID

    CPT 82042: Albumin Quantitation in Non-Blood Body Fluid

    CPT-82043-URINE-ALBUMIN-QUANTITATION-KIDNEY-DISEASE

    CPT 82043: Urine Albumin Quantitative Assay

    Showing 1–10 of 411

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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