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    v1.0.28
    OpenPayerBilling CodesChemistry ProceduresCPT cpt-82261-biotinidase-enzyme-assay-blood
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    CPT 82261: Biotinidase Enzyme Assay

    CPT code 82261 describes a laboratory measurement of the enzyme biotinidase, typically performed on a blood sample to identify biotinidase deficiency or to follow up a previously positive newborn screen; the service is a clinical chemistry/diagnostic laboratory test usually provided in a clinical laboratory or hospital laboratory setting and is reported by laboratories performing the enzymatic assay.

    For related coverage guidance, see recent payer policy updates: Salivary Hormone Testing, Parathyroid Hormone, Phosphorus, Calcium, and Magnesium Testing, Bone Turnover Markers Testing.

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    Clinical & Coding Specifications

    Clinical Context

    A newborn screening or outpatient metabolic evaluation patient presents after a positive newborn screen for biotinidase deficiency or with clinical signs such as unexplained seizures, feeding intolerance, hypotonia, or developmental delay. A clinician orders quantitative measurement of the enzyme biotinidase on a blood sample (usually serum or dried blood spot) to confirm deficiency. The clinical workflow includes specimen collection (venipuncture or heel stick), proper labeling and transport to a clinical laboratory, accessioning, and performance of the enzymatic assay by a laboratory technologist or clinical chemist. Results are reviewed by a pathologist or laboratory director and communicated to the ordering pediatrician, geneticist, or metabolic specialist for diagnostic confirmation, family counseling, and management decisions such as biotin supplementation and further genetic testing if indicated.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80055General health panel (includes metabolic screening tests)May be ordered concurrently as part of broader metabolic evaluation in an ill infant.
    80400Phenylketonuria (PKU) screening — quantitative (example of newborn metabolic screen component)Often part of the newborn screening program alongside biotinidase testing in population screening workflows.
    81406Assay of multiple gene sequences (targeted sequence analysis, example)May be used subsequently for confirmatory genetic testing of the BTD gene if enzyme deficiency is detected.
    36415Collection of venous blood by venipunctureCommonly performed to obtain serum for the biotinidase assay when heel stick is not used.
    88172Cytopathology/flow — note: not directly relatedData not available in the input; included only when clinically necessary.

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207Q00000XFamily Medicine PhysicianFamily Medicine is the medical specialty which is concerned with the total health care of the individual and the family. It is the specialty in breadt…
    208M00000XHospitalist PhysicianHospitalists are physicians whose primary professional focus is the general medical care of hospitalized patients. Their activities include patient ca…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    208D00000XGeneral Practice PhysicianA physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
    363LX0106XOccupational Health Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner
    163WP0218XPediatric Oncology Registered NurseNursing Service Providers · Registered Nurse
    341600000XAmbulanceAn emergency vehicle used for transporting patients to a health care facility after injury or illness. Types of ambulances used in the United States i…

    Related ICD-10-CM Codes

    25 rows

    ICD-10-CM CodeDescription
    E44.0Moderate protein-calorie malnutrition
    E44.1Mild protein-calorie malnutrition
    E67.8Other specified hyperalimentation
    E70.0Classical phenylketonuria
    E71.0Maple-syrup-urine disease
    E83.39Other disorders of phosphorus metabolism
    F50.9Eating disorder, unspecified
    F98.4Stereotyped movement disorders
    G40.309Generalized idiopathic epilepsy and epileptic syndromes, not intractable, without status epilepticus
    G40.401Other generalized epilepsy and epileptic syndromes, not intractable, with status epilepticus

    Showing 1–10 of 25

    Related Payer Policy Updates

    Specialty: Endocrinology1 / 3

    10 rows

    PolicyPayerEffective Date
    Salivary Hormone TestingOscar Health2026-10-01
    Parathyroid Hormone, Phosphorus, Calcium, and Magnesium TestingOscar Health2026-10-01
    Bone Turnover Markers TestingOscar Health2026-10-01
    Testosterone — Coverage Criteria for Measurement and MonitoringOscar Health2026-10-01
    Diabetes Mellitus Testing — Coverage CriteriaOscar Health2026-10-01
    Thyroid Disease TestingOscar Health2026-10-01
    Thyroid Disease Testingblue cross blue shield - louisiana2026-09-15
    Thyroid Disease TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Parathyroid Hormone, Phosphorous, Calcium and Magnesium TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Testosterone laboratory testing and managementPremera Bluecross2026-09-04

    Related Codes

    CPT-82009-KETONE-BODIES-QUALITATIVE-TEST

    CPT 82009: Qualitative Ketone Bodies Test

    CPT-82010-QUANTITATIVE-KETONE-BODIES-ASSAY-SERUM-PLASMA

    CPT 82010: Quantitative Ketone Bodies in Serum or Plasma

    CPT-82013-ACETYLCHOLINESTERASE-QUANTITATIVE-ASSAY

    CPT 82013: Quantitative Acetylcholinesterase (Acetylhydrolase) Assay

    CPT-82016-QUALITATIVE-ACYLCARNITINES-METABOLIC-SCREEN

    CPT 82016: Qualitative Acylcarnitines, Plasma/Serum/Urine

    CPT-82017-QUANTITATIVE-ACYLCARNITINE-ANALYSIS

    CPT 82017: Quantitative Acylcarnitine Analysis

    CPT-82024-QUANTITATIVE-CORTICOTROPIN-ACTH-ASSAY

    CPT 82024: Adrenocorticotropic Hormone (ACTH) Quantitative Assay

    CPT-82030-QUANTITATIVE-CAMP-PLASMA-URINE-ASSAY

    CPT 82030: Quantitative cyclic AMP assay on plasma or urine

    CPT-82040-QUANTITATIVE-ALBUMIN-MEASUREMENT

    CPT 82040: Quantitative Albumin Measurement

    CPT-82042-QUANTITATIVE-ALBUMIN-NONBLOOD-FLUID

    CPT 82042: Albumin Quantitation in Non-Blood Body Fluid

    CPT-82043-URINE-ALBUMIN-QUANTITATION-KIDNEY-DISEASE

    CPT 82043: Urine Albumin Quantitative Assay

    Showing 1–10 of 411

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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