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    v1.0.28
    OpenPayerBilling CodesAutografts/Tissue Cultured AutograftCPT cpt-15040-skin-harvest-tissue-cultured-autograft
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    CPT 15040: Harvest of Skin for Tissue Cultured Skin Autograft (≤100 cm2)

    Medicare pays $283 on average nationally for this procedure.

    CPT code 15040 describes harvesting up to 100 cm2 of skin for a tissue cultured skin autograft, a surgical skin harvest procedure typically performed in an operating room or procedure suite as part of skin grafting and reconstructive care; the service involves obtaining a small skin specimen that will be processed for cultured epithelial autograft preparation and is classified as a surgical harvesting procedure rather than a standalone graft application.

    For related coverage guidance, see recent payer policy updates: Onychomycosis diagnostic testing and coverage, Topical Acne-Non-Retinoid Products, Dermatology - Vtama Drug Quantity Management Policy - Per Days.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A typical patient for 15040 is an adult with a full-thickness or deep partial-thickness burn, chronic non-healing wound, or extensive traumatic skin loss who requires tissue cultured skin autograft harvested from a donor site measuring 100 cm2 or less. The clinical workflow begins with initial wound assessment in an acute care or outpatient specialty setting (burn center, plastic surgery clinic, or wound care center). Preoperative evaluation includes wound bed preparation (debridement, infection control), informed consent, and medical optimization. On the day of service the patient is brought to an operating room or procedure suite; the provider harvests a small split-thickness skin sample (≤100 cm2) which is then sent to a laboratory for culture and expansion into autologous cultured epithelial autograft. The donor site is dressed and the patient is monitored for donor and recipient site complications. Follow-up visits include graft application when cultured product is ready, wound assessments, and rehabilitation. Typical sites of service are hospital outpatient departments, ambulatory surgical centers, and specialized burn centers. Common payors include Aetna, Blue Cross Blue Shield, Cigna Health, UnitedHealthcare, BUCA, and Medicare.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    11042Debridement, subcutaneous tissue (includes epidermis and dermis), first 20 sq cm or lessOften performed prior to harvesting skin for cultured autograft to prepare a healthy wound bed.
    15002Harvest of skin autograft, first 25 sq cm or less, or as specified (split-thickness)Alternative/adjacent harvesting codes for larger or different graft types; used when immediate autograft is applied rather than cultured product.
    15271Application of skin substitute graft to trunk, arms, legs; first 100 sq cm or lessMay be used when applying cultured skin substitute product to the recipient site once available.
    15740Muscle, myocutaneous, or fasciocutaneous flap; trunkUsed when more complex reconstruction beyond cultured autograft is required.
    99499Unlisted evaluation and management serviceUsed for billing atypical perioperative care not captured by standard E/M codes when payor allows.

    Related Modifiers

    12 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    23Modifier -23 (Unusual Anesthesia) is used on anesthesia service CPT codes when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia…- Use modifier -23 only on anesthesia CPT codes (00100–01999) when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia because of…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    50Modifier –50 (Bilateral Procedure) is appended to a CPT code when an identical procedure is performed on paired anatomical structures during the same operative session. The AMA states to add…- Use modifier -50 when the same CPT surgical, diagnostic, or radiology procedure is performed on paired anatomical structures (both sides of the body) during the same operative/session by the same…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    66Modifier 66 (Surgical Team) is used when three or more surgeons act as primary surgeons on a single, highly complex procedure. The AMA defines it for use when a surgical team — often composed of…- Use modifier 66 when three or more surgeons act as primary surgeons working collaboratively as a surgical team on a single, highly complex or difficult procedure (for example organ…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…

    Showing 1–10 of 12

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207P00000XEmergency Medicine PhysicianAn emergency physician focuses on the immediate decision making and action necessary to prevent death or any further disability both in the pre-hospit…
    2080P0208XPediatric Infectious Diseases PhysicianA pediatrician trained to care for children in the diagnosis, treatment and prevention of infectious diseases. This specialist can apply specific know…
    213E00000XPodiatristA podiatrist is a person qualified by a Doctor of Podiatric Medicine (D.P.M.) degree, licensed by the state, and practicing within the scope of that l…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    364S00000XClinical Nurse SpecialistA registered nurse who, through a graduate degree program in nursing, or through a formal post-basic education program or continuing education courses…
    207VM0101XMaternal & Fetal Medicine PhysicianAn obstetrician/gynecologist who cares for, or provides consultation on, patients with complications of pregnancy. This specialist has advanced knowle…
    2081N0008XNeuromuscular Medicine (Physical Medicine & Rehabilitation) PhysicianA physician who specializes in neuromuscular medicine possesses specialized knowledge in the science, clinical evaluation and management of these diso…
    251T00000XPACE Provider OrganizationA PACE provider organization is a not-for-profit private or public entity that is primarily engaged in providing PACE services(unique capitated manage…

    Related ICD-10-CM Codes

    15 rows

    ICD-10-CM CodeDescription
    B35.1Tinea unguium
    C44.311Basal cell carcinoma of skin of nose
    E10.621Type 1 diabetes mellitus with foot ulcer
    E11.621Type 2 diabetes mellitus with foot ulcer
    I83.90Asymptomatic varicose veins of unspecified lower extremity
    K13.21Leukoplakia of oral mucosa, including tongue
    L90.5Scar conditions and fibrosis of skin
    L97.405Non-pressure chronic ulcer of unspecified heel and midfoot with muscle involvement without evidence of necrosis
    L97.412Non-pressure chronic ulcer of right heel and midfoot with fat layer exposed
    L98.9Disorder of the skin and subcutaneous tissue, unspecified

    Showing 1–10 of 15

    Related Payer Policy Updates

    Specialty: Dermatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Onychomycosis diagnostic testing and coverageOscar Health2026-10-01
    Topical Acne-Non-Retinoid ProductsCigna2026-08-15
    Dermatology - Vtama Drug Quantity Management Policy - Per DaysCigna2026-08-15
    Medical Necessity Guidelines: Acne and Rosacea Medicationsharvard pilgrim health care2026-08-11
    Pharmacologic Treatment of PsoriasisPremera Bluecross2026-08-01
    Winlevi (clascoterone 1% cream) — Topical AcneCigna2026-08-01
    Spevigo (spesolimab‑sbzo) for generalized pustular psoriasisUnitedHealthcare2026-08-01
    Onychomycosis Testingblue cross blue shield - louisiana2026-08-01
    Spesolimab-sbzo (Spevigoblue cross blue shield - tennessee2026-07-31
    Home ultraviolet light therapy equipment for skin conditionsselect_health2026-07-01

    Related Codes

    CPT-15050-PINCH-GRAFT-SKIN-GRAFTING-SMALL-DEFECT

    CPT 15050: Pinch Graft, Skin, Up to 2 cm

    CPT-15100-SPLIT-THICKNESS-AUTOGRAFT-TRUNK-ARMS-LEGS

    CPT 15100: Split-Thickness Autograft from Trunk/Arms/Legs

    CPT-15101-SPLIT-THICKNESS-AUTOGRAFT-ADDITIONAL-100CM2

    CPT 15101: Split-Thickness Autograft, Additional 100 cm²

    CPT-15110-EPIDERMAL-AUTOGRAFT-TRUNK-ARMS-LEGS

    CPT 15110: Epidermal Autograft, Trunk/Arms/Legs

    CPT-15111-EPIDERMAL-AUTOGRAFT-TRUNK-ARMS-LEGS-ADDITIONAL

    CPT 15111: Epidermal Autograft, Trunk/Arms/Legs (per 100 cm2)

    CPT-15115-EPIDERMAL-AUTOGRAFT-FACE-AND-SENSITIVE-AREAS

    CPT 15115: Epidermal Autograft for Face/Scalp/Hands/Feet

    CPT-15116-EPIDERMAL-AUTOGRAFT-FACE-SCALP-HANDS-FEET

    CPT 15116: Epidermal Autograft for Face, Hands, Feet, Multiple Digits

    CPT-15120-SPLIT-THICKNESS-AUTOGRAFT-FACE-HANDS-FEET

    CPT 15120: Split‑Thickness Autograft, Face/Scalp (first 100 sq cm)

    CPT-15121-SPLIT-THICKNESS-AUTOGRAFT-ADDITIONAL-100-SQ-CM

    CPT 15121: Split-Thickness Autograft for Face, Scalp, Eyelids, Mouth, Neck, Ears

    CPT-15130-DERMAL-AUTOGRAFT-HARVEST-PLACEMENT-TRUNK-ARMS-LEGS

    CPT 15130: Dermal Autograft Harvest and Placement

    Showing 1–10 of 27

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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