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CPT 88368: In Situ Hybridization Single Probe with Pathologist Evaluation
CPT code 88368 covers in situ hybridization (single probe) testing where a lab analyst performs the initial stain on slide(s) derived from a single patient specimen and a qualified provider—typically a pathologist—evaluates the stained slides, including manual counting or estimation of probe number and location. This code is central to molecular pathology workflows for tumor and other tissue-based diagnostics and supports clinical decision-making for targeted therapies and prognostic assessment. Nationally, accurate coding of 88368 ensures appropriate recognition of the professional interpretive component of single-probe ISH testing and aligns billing with laboratory and pathology service delivery.
Key payers referenced in the analysis include Aetna, Blue Cross Blue Shield, Cigna Health, UnitedHealthcare, and Medicare. Readers will find a concise overview of the code’s clinical role, expected sites of service, and common billing considerations. The publication outlines benchmark metrics for utilization and reimbursement where available, notes relevant policy updates affecting molecular pathology reporting, and situates 88368 within related pathology service lines. Data not available in the input will be identified as such in the detailed sections. The coverage is intended for a national audience of health policy analysts, billing professionals, and clinical laboratory leaders.
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Billing Code Overview
CPT code 88368 describes an in situ hybridization (single probe) procedure in which a laboratory analyst performs the initial staining on slide(s) prepared from a single patient specimen, commonly tumor tissue. A qualified provider, typically a pathologist, subsequently evaluates the stained slide(s), which includes manual counting or estimation of probe number and location.
Service type: Laboratory pathology procedure — in situ hybridization (single probe) with professional interpretation and manual probe counting.
Typical site of service: Hospital pathology laboratory, independent pathology or molecular diagnostics laboratory, or outpatient surgical pathology lab where tumor or other tissue specimens are prepared and reviewed by a pathologist.