CPT code 88367 covers in situ hybridization (ISH) single probe staining performed on slide(s) from a single patient specimen with computer-assisted enumeration and subsequent pathologist interpretation. This code captures a combined laboratory workflow where a trained lab analyst performs the initial staining and digital or automated counts, and a qualified provider issues the diagnostic interpretation. Nationally, ISH testing supports diagnosis, prognostication, and therapeutic decision-making in oncology and other specialty pathology areas, making accurate coding essential for clinical documentation and payment integrity.

Key payers addressed in this analysis include Aetna, Blue Cross Blue Shield, Cigna Health, UnitedHealthcare, and Medicare. Readers will find a practical overview of what 88367 represents, benchmarks and payer coverage patterns where available, coding and billing considerations tied to the service description, and clinical context for use in tumor tissue evaluation. Data not available in the input will be flagged explicitly. The publication focuses on policy and billing implications at a national level and provides reference points for laboratories, pathology groups, and compliance teams seeking concise guidance on the code's clinical and administrative role.