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    v1.0.30
    OpenPayerBilling CodesSurgical Pathology ProceduresCPT cpt-88311-decalcification-specimen-preparation
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    CPT 88311: Decalcification Procedure for Tissue Specimens

    Medicare pays $14 on average nationally for this procedure.

    CPT code 88311 describes a laboratory decalcification procedure in which a lab analyst dissolves calcium from a tissue or specimen to facilitate microscopic examination; this is a pathology/laboratory service typically performed in a hospital or independent clinical laboratory setting to enable histologic processing and diagnostic review.

    For related coverage guidance, see recent payer policy updates: Destruction of Pre-Malignant Skin Lesions, Onychomycosis diagnostic testing and coverage, Treatment of Capillary Malformations (Port Wine Stains).

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    Clinical & Coding Specifications

    Clinical Context

    A 62-year-old female presents after surgical excision of a portion of her mandible for a chronic osteomyelitis lesion. The surgical specimen includes bone fragments that require histologic evaluation for infection and tumor exclusion. The pathology laboratory receives the bony specimen and documents requests for routine H&E staining and possible special stains. Because bone is heavily mineralized, the histology technician performs a decalcification procedure to dissolve calcium salts and soften the specimen, enabling proper sectioning, embedding, and microscopic evaluation. The typical workflow: accessioning and labeling of the specimen, gross description by the pathologist, fixation in formalin, decalcification using acid or chelating agents, processing, embedding, microtomy, staining, and pathologist interpretation. This service is performed in the anatomic pathology laboratory setting associated with hospital-based or independent outpatient surgical pathology services. Common clinical scenarios include evaluation of bone removed for infection (osteomyelitis), benign or malignant bone tumors, dental/mandibular resections, and bone biopsy specimens for metabolic or inflammatory bone disease.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    88305Level IV surgical pathology, gross and microscopic examinationCommonly billed for the diagnostic microscopic interpretation performed after decalcification and slide preparation.
    88312Decalcification, for bone marrow or bone specimens; with additional consultation or special staining (Note: 88311 is decalcification alone)Billed when decalcification includes additional steps or special processing beyond standard decalcification.
    88342Immunohistochemistry, per single antibody stainMay be performed after decalcification to characterize tumor markers or infectious agents on bone tissue.
    88331Frozen section; preparation and interpretationPerformed intraoperatively prior to decalcification when immediate diagnosis is required; decalcification occurs later for permanent sections.
    88302Level I surgical pathology, gross onlyBilled when only gross description is provided and no microscopic examination is performed because specimen is entirely decalcified and non-diagnostic.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    51Modifier –51 (Multiple Procedures) is used when the same provider performs multiple procedures in the same session to identify secondary, non‑exempt procedures and indicate payment reduction logic…- Use modifier -51 when the same provider performs multiple distinct procedures during the same patient encounter: report the highest‑valued procedure first (no modifier) and append -51 to…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RC0000X
    363A00000X
    207L00000X
    364SX0204X
    207QP0002X
    2084P0015X
    156FX1700X
    207WX0110X

    Related ICD-10-CM Codes

    25 rows

    ICD-10-CM CodeDescription
    C41.9Malignant neoplasm of bone and articular cartilage, unspecified
    C79.51Secondary malignant neoplasm of bone
    D16.9Benign neoplasm of bone and articular cartilage, unspecified
    D48.5Neoplasm of uncertain behavior of skin
    K80.10Calculus of gallbladder with chronic cholecystitis without obstruction
    M80.08XAAge-related osteoporosis with current pathological fracture, vertebra(e), initial encounter for fracture
    M80.88XAOther osteoporosis with current pathological fracture, vertebra(e), initial encounter for fracture
    M84.50XAPathological fracture in neoplastic disease, unspecified site, initial encounter for fracture
    M84.571APathological fracture in neoplastic disease, right ankle, initial encounter for fracture
    M84.572APathological fracture in neoplastic disease, left ankle, initial encounter for fracture

    Showing 1–10 of 25

    Related Payer Policy Updates

    Specialty: Dermatology1 / 3

    10 rows

    PolicyPayerEffective Date
    Destruction of Pre-Malignant Skin LesionsAnthem2026-10-01
    Onychomycosis diagnostic testing and coverageOscar Health2026-10-01
    Treatment of Capillary Malformations (Port Wine Stains)universityofutahhealthplans2026-09-28
    AnzupgoCigna2026-09-15
    Zevaskyn Coverage for Recessive Dystrophic Epidermolysis BullosaCigna2026-09-01
    Topical Acne-Non-Retinoid ProductsCigna2026-08-15
    Dermatology - Vtama Drug Quantity Management Policy - Per DaysCigna2026-08-15
    Medical Necessity Guidelines: Acne and Rosacea Medicationsharvard pilgrim health care2026-08-11
    Dermatology - Anzupgo Prior Authorization PolicyCigna2026-08-05
    Light Therapy for Dermatologic Conditionsbcbsvermont2026-08-01

    Related Codes

    CPT-88300-LEVEL-I-SURGICAL-PATHOLOGY-GROSS-EXAMINATION-ACCESSIONING

    CPT 88300: Surgical Pathology, Level I Gross Examination

    CPT-88302-SURGICAL-PATHOLOGY-LEVEL-II-GROSS-AND-MICROSCOPIC-EXAMINATION

    CPT 88302: Surgical Pathology, Level II Gross and Microscopic Exam

    CPT-88304-SURGICAL-PATHOLOGY-LEVEL-III-GROSS-AND-MICROSCOPIC

    CPT 88304: Surgical Pathology, Level III Examination

    CPT-88305-SURGICAL-PATHOLOGY-LEVEL-IV-EXAMINATION

    CPT 88305: Surgical Pathology, Gross and Microscopic Examination, Level IV

    CPT-88307-SURGICAL-PATHOLOGY-LEVEL-V-EXAMINATION

    CPT 88307: Surgical Pathology Level V Examination

    CPT-88309-LEVEL-VI-SURGICAL-PATHOLOGY-EXAMINATION

    CPT 88309: Level VI Surgical Pathology Examination

    CPT-88312-SPECIAL-STAIN-MICROORGANISMS-TECHNICAL-COMPONENT

    CPT 88312: Special Stain, Technical Component for Microorganisms

    CPT-88313-SPECIAL-STAIN-TECHNICAL-COMPONENT

    CPT 88313: Technical Special Stain for Group II (e.g., iron, trichrome)

    CPT-88314-FROZEN-TISSUE-HISTOCHEMICAL-SPECIAL-STAIN

    CPT 88314: Frozen Tissue Histochemical Special Stain, Interpretation

    CPT-88319-ENZYME-SPECIAL-STAIN-PATHOLOGY-INTERPRETATION

    CPT 88319: Special Stain for Enzyme Constituents, With Interpretation

    Showing 1–10 of 47

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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