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    v1.0.30
    OpenPayerBilling CodesVirus Isolation ProceduresCPT cpt-87252-viral-culture-identification-tissue-culture
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    CPT 87252: Viral Isolation in Tissue Culture

    CPT code 87252 describes laboratory viral isolation by inoculating a known cell line tissue culture, allowing the virus to propagate, and identifying the virus through observation of cytopathic effects; this is a viral isolation in tissue culture service typically performed by a clinical microbiology or virology laboratory and commonly provided in an inpatient or outpatient hospital laboratory or reference laboratory setting.

    For related coverage guidance, see recent payer policy updates: Long-Acting Injectable Antiretroviral Agents for HIV, B-Hemolytic Streptococcus Testing, Human Immunodeficiency Virus (HIV) — Coverage Criteria for Testing and Resistance Assays.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 6-year-old child presents to a hospital pediatric emergency department with 3 days of fever, cough, and increasing respiratory distress. A nasopharyngeal swab and throat specimen are collected and sent to the hospital virology laboratory. The laboratory technologist inoculates a tissue culture of a known susceptible cell line with the patient specimen, incubates the culture, and monitors for cytopathic effects. When viral growth is observed, the lab analyst performs identification (eg, neutralization, immunofluorescence, or PCR on culture material) to confirm the specific viral etiology. Results are reported to the ordering clinician and documented in the electronic medical record; public health notifications occur when appropriate.

    Typical site of service: hospital clinical virology laboratory or reference microbiology laboratory performing diagnostic viral culture.

    Typical service type: laboratory diagnostic procedure involving viral isolation in tissue culture and identification.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    87252Culture, viral; tissue, shell vial culture, inoculation and diagnostic identificationPrimary code describing viral isolation by inoculating tissue culture and identifying virus via cytopathic effects.
    87480Infectious agent antigen detection by immunoassay technique, influenza, eachPerformed on direct specimen or culture material to rapidly detect influenza antigens; may precede or follow culture.
    87536Infectious agent detection by nucleic acid (DNA or RNA); influenza A and B, multiplexMolecular testing commonly performed alongside or instead of culture for faster viral identification; culture may be used for confirmation or surveillance.
    87804Infectious agent antigen detection by immunoassay technique, respiratory syncytial virusRapid antigen testing that may be performed on the original specimen; culture used for definitive identification or further characterization.
    87140Culture, bacterial; any other source except urine, blood or stool, aerobic isolateBacterial cultures that may be ordered concurrently when bacterial coinfection is suspected; different lab workflow but often part of the infectious workup.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207RH0000X
    207L00000X
    174400000X
    207V00000X
    225CA2500X
    163WR0400X
    207ZC0008X
    282N00000X

    Related ICD-10-CM Codes

    27 rows

    ICD-10-CM CodeDescription
    A49.9Bacterial infection, unspecified
    A71.9Trachoma, unspecified
    A75.9Typhus fever, unspecified
    A83.0Japanese encephalitis
    A87.9Viral meningitis, unspecified
    B00.9Herpesviral infection, unspecified
    B02.9Zoster without complications
    B05.9Measles without complication
    B26.9Mumps without complication
    B30.9Viral conjunctivitis, unspecified

    Showing 1–10 of 27

    Related Payer Policy Updates

    Specialty: Infectious Disease1 / 3

    10 rows

    PolicyPayerEffective Date
    Long-Acting Injectable Antiretroviral Agents for HIVColorado Rocky Mountain Health Plans2026-10-01
    B-Hemolytic Streptococcus TestingOscar Health2026-10-01
    Human Immunodeficiency Virus (HIV) — Coverage Criteria for Testing and Resistance AssaysOscar Health2026-10-01
    Hepatitis Testing (A, B, C, D) Coverage CriteriaOscar Health2026-10-01
    Lyme Disease Testing Coverage CriteriaOscar Health2026-10-01
    Pathogen Panel TestingOscar Health2026-10-01
    Diagnostic Testing of Common Sexually Transmitted InfectionsOscar Health2026-10-01
    Coronavirus Testing in the Outpatient SettingOscar Health2026-10-01
    Testing for Vector-Borne InfectionsOscar Health2026-10-01
    Testing for Diagnosis of Active or Latent TuberculosisOscar Health2026-10-01

    Related Codes

    CPT-87250-VIRAL-ISOLATION-IN-EMBRYONATED-EGG-OR-SMALL-ANIMAL

    CPT 87250: Viral Isolation Using Embryonated Egg or Small Animal

    CPT-87253-VIRAL-IDENTIFICATION-AFTER-TISSUE-CULTURE

    CPT 87253: Virus Identification by Neutralization After Tissue Culture

    CPT-87254-SHELL-VIAL-VIRAL-CULTURE-IMMUNOFLUORESCENCE

    CPT 87254: Shell Vial Viral Culture with Immunofluorescence

    CPT-87255-VIRAL-IDENTIFICATION-CELL-IMPACT-ANALYSIS

    CPT 87255: Viral Identification by Cytopathic Effect, Non‑Immunologic

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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