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    v1.0.28
    OpenPayerBilling CodesQualitative or Semiquantitative ImmunoassaysCPT cpt-86665-ebv-vca-antibody-immunoassay-serum
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    CPT 86665: EBV VCA Antibody Immunoassay

    CPT code 86665 describes an immunoassay performed by a laboratory analyst to detect serum antibodies to the Epstein–Barr virus viral capsid antigen (VCA), a serologic test used to evaluate EBV exposure or immune status; service type: laboratory serology/antibody testing; typical site of service: clinical laboratory or hospital laboratory processing patient serum specimens.

    For related coverage guidance, see recent payer policy updates: Diagnosis of Idiopathic Environmental Intolerance, Allergen Testing, Allergen Testing.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 19-year-old college student presents to an urgent care clinic with 5 days of fever, sore throat, fatigue, and cervical lymphadenopathy. The clinician performs a physical exam and orders laboratory testing to evaluate for infectious mononucleosis. A phlebotomy technician collects a serum sample which is sent to the hospital or reference laboratory. The laboratory analyst performs an immunoassay to detect antibodies to Epstein–Barr virus viral capsid antigen (VCA IgM and/or IgG) using automated immunoassay platforms. Results are reported to the ordering clinician, who uses the serology pattern (VCA IgM positive, VCA IgG positive/negative, and other EBV markers if available) to determine acute versus past EBV infection and to guide clinical counseling and follow-up. Typical sites of service include outpatient clinic, urgent care, emergency department, and independent/reference laboratories. The procedure is a laboratory diagnostic test performed by clinical laboratory personnel; specimen handling, accessioning, analysis, and result verification are part of the clinical workflow associated with this CPT-coded immunoassay.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    36415Collection of venous blood by venipunctureVenipuncture is commonly performed prior to sending serum for the EBV VCA immunoassay.
    80053Comprehensive metabolic panelOften ordered concurrently to assess liver function in patients with suspected infectious mononucleosis (elevated transaminases).
    86769Hepatitis C antibody; confirmatory immunoassayExample of another serologic immunoassay workflow in the laboratory; similar processing and reporting steps.
    86664Cytomegalovirus (CMV) antibody serology; immunoassay for CMV IgM or IgGCMV serologies are commonly ordered in the differential diagnosis of viral syndromes and can be run alongside EBV serology.
    86336Antibody; EBV nuclear antigen (EBNA)EBNA testing is commonly used with VCA results to distinguish acute from past EBV infection; often ordered as part of the EBV serologic panel.

    Related Modifiers

    6 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207R00000XInternal Medicine PhysicianA physician who provides long-term, comprehensive care in the office and the hospital, managing both common and complex illness of adolescents, adults…
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    208D00000XGeneral Practice PhysicianA physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
    103T00000XPsychologistA psychologist is an individual who is licensed to practice psychology which is defined as the observation, description, evaluation, interpretation, a…
    2255A2300XAthletic TrainerAthletic trainers are allied health care professionals who work in consultation with or under the direction of physicians, and specialize in the preve…
    2084B0040XBehavioral Neurology & Neuropsychiatry PhysicianBehavioral Neurology & Neuropsychiatry is a medical subspecialty involving the diagnosis and treatment of neurologically based behavioral issues.
    224ZL0004XLow Vision Occupational Therapy AssistantOccupational therapy assistants contribute to the completion of an individualized occupational therapy low-vision evaluation under the direction and s…
    2084P0802XAddiction Psychiatry PhysicianAddiction Psychiatry is a subspecialty of psychiatry that focuses on evaluation and treatment of individuals with alcohol, drug, or other substance-re…

    Related ICD-10-CM Codes

    26 rows

    ICD-10-CM CodeDescription
    B27.90Infectious mononucleosis, unspecified without complication
    B27.91Infectious mononucleosis, unspecified with polyneuropathy
    B34.9Viral infection, unspecified
    B95.8Unspecified staphylococcus as the cause of diseases classified elsewhere
    F32.9Major depressive disorder, single episode, unspecified
    F41.9Anxiety disorder, unspecified
    H92.09Otalgia, unspecified ear
    J02.9Acute pharyngitis, unspecified
    J06.9Acute upper respiratory infection, unspecified
    K52.9Noninfective gastroenteritis and colitis, unspecified

    Showing 1–10 of 26

    Related Payer Policy Updates

    Specialty: Allergy/Clinical Immunology1 / 3

    9 rows

    PolicyPayerEffective Date
    Diagnosis of Idiopathic Environmental IntoleranceOscar Health2026-10-01
    Allergen Testingblue cross blue shield - louisiana2026-09-15
    Allergen TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Hereditary Angioedema - EkterlyCigna2026-08-15
    Omalizumab products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab Products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab ProductsPremera Bluecross2026-08-01
    Allergy Testinguniverahealthcare2026-07-16
    Omalizumab (Xolair) and related biologics coverageMass General Brigham Health Plan2026-07-01

    Related Codes

    86803-QUALITATIVE-OR-SEMIQUANTITATIVE-IMMUNOASSAYS-HEPATITIS-C

    CPT 86803: Hepatitis C Antibody Immunoassay in Serum

    CPT-86000-FEBRILE-AGGLUTININS-SEROLOGIC-TEST

    CPT 86000: Febrile Agglutinins (Serologic Agglutination) Test

    CPT-86001-ALLERGEN-SPECIFIC-IGG-LABORATORY-TEST

    CPT 86001: Allergen-Specific IgG Assay, Technical Component

    CPT-86003-ALLERGEN-SPECIFIC-IGE-ASSAY-TECHNICAL

    CPT 86003: Allergen‑Specific IgE Assay Using Crude Allergen Extract

    CPT-86005-MULTI-ALLERGEN-IGE-SCREENING-QUALITATIVE

    CPT 86005: Multi-Allergen Allergen-Specific IgE Screening

    CPT-86008-ALLERGEN-COMPONENT-SPECIFIC-IGE-ASSAY

    CPT 86008: Allergen Component–Specific IgE Assay

    CPT-86015-ANTI-ACTIN-SMOOTH-MUSCLE-ANTIBODY-ASMA

    CPT 86015: Actin (Smooth Muscle) Antibody (ASMA) Immunoassay

    CPT-86021-LEUKOCYTE-ANTIBODY-IDENTIFICATION-LAB-TECHNICAL

    CPT 86021: Leukocyte Antibody Identification Laboratory Test

    CPT-86022-PLATELET-ANTIBODY-IDENTIFICATION-TECHNICAL-TEST

    CPT 86022: Platelet Antibody Detection and Identification

    CPT-86023-PLATELET-ASSOCIATED-IMMUNOGLOBULIN-DETECTION

    CPT 86023: Platelet-Associated Immunoglobulin Assay

    Showing 1–10 of 199

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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