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    v1.0.28
    OpenPayerBilling CodesQualitative or Semiquantitative ImmunoassaysCPT cpt-86320-immunoelectrophoresis-serum-immunoglobulin-evaluation
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    CPT 86320: Immunoelectrophoresis (Serum Immunoglobulin Evaluation)

    Medicare pays $18 on average nationally for this procedure.

    CPT code 86320 describes immunoelectrophoresis (IEP) performed by a laboratory analyst to evaluate a patient’s serum for the amounts and types of immunoglobulins (antibodies); this is a laboratory diagnostic service typically performed in a clinical laboratory or hospital laboratory setting to assess immune protein patterns and detect monoclonal or abnormal protein bands.

    For related coverage guidance, see recent payer policy updates: Diagnosis of Idiopathic Environmental Intolerance, Allergen Testing, Allergen Testing.

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    Clinical & Coding Specifications

    Clinical Context

    A typical scenario involves an adult outpatient referred by a primary care physician or hematologist for evaluation of a suspected gammopathy. The patient presents with unexplained anemia, recurrent infections, hypercalcemia, bone pain, or an abnormal serum protein/total protein or electrophoresis screen. A venous blood specimen is collected in the phlebotomy area and sent to the clinical laboratory. The laboratory technologist performs serum immunoelectrophoresis (86320) to separate and identify immunoglobulin classes and abnormal monoclonal bands. Results are reported to the ordering clinician and used alongside serum protein electrophoresis, quantitative immunoglobulin levels, and clinical findings to diagnose monoclonal gammopathy, multiple myeloma, Waldenström macroglobulinemia, or immune deficiency states. Typical sites of service include hospital outpatient laboratories, independent clinical reference laboratories, and hospital inpatient laboratories when inpatient evaluation is required.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80053Comprehensive metabolic panelBaseline metabolic evaluation often ordered with serum protein studies to assess kidney and calcium abnormalities in suspected myeloma
    84155Protein; totalProvides total serum protein used with electrophoresis to assess abnormal protein levels
    84439Assay of parathyroid hormone (PTH) or specific endocrine tests (Note: related endocrine tests vary)Used when hypercalcemia is present and evaluation of calcium regulation is needed
    85025Complete blood count (CBC) with automated differentialEvaluates anemia or cytopenias that may accompany plasma cell disorders
    86301Antibody identification, non-specific (Note: related immunologic testing)Complementary immunologic testing that may be ordered depending on clinical context

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    23Modifier -23 (Unusual Anesthesia) is used on anesthesia service CPT codes when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia…- Use modifier -23 only on anesthesia CPT codes (00100–01999) when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia because of…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207RC0000XCardiovascular Disease PhysicianAn internist who specializes in diseases of the heart and blood vessels and manages complex cardiac conditions such as heart attacks and life-threaten…
    207RG0300XGeriatric Medicine (Internal Medicine) PhysicianAn internist who has special knowledge of the aging process and special skills in the diagnostic, therapeutic, preventive and rehabilitative aspects o…
    208D00000XGeneral Practice PhysicianA physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
    207L00000XAnesthesiology PhysicianAn anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
    363A00000XPhysician AssistantA physician assistant is a person who has successfully completed an accredited education program for physician assistant, is licensed by the state and…
    163WR0400XRehabilitation Registered NurseNursing Service Providers · Registered Nurse
    207QA0000XAdolescent Medicine (Family Medicine) PhysicianA family medicine physician with multidisciplinary training in the unique physical, psychological and social characteristics of adolescents and their …
    261QE0800XEndoscopy Clinic/CenterAmbulatory Health Care Facilities · Clinic/Center

    Related ICD-10-CM Codes

    27 rows

    ICD-10-CM CodeDescription
    A41.9Sepsis, unspecified organism
    B20Human immunodeficiency virus [HIV] disease
    C83.30Diffuse large B-cell lymphoma, unspecified site
    C90.00Multiple myeloma not having achieved remission
    D50.9Iron deficiency anemia, unspecified
    D63.0Anemia in neoplastic disease
    D69.6Thrombocytopenia, unspecified
    D80.0Hereditary hypogammaglobulinemia
    D83.9Common variable immunodeficiency, unspecified
    D89.9Disorder involving the immune mechanism, unspecified

    Showing 1–10 of 27

    Related Payer Policy Updates

    Specialty: Allergy/Clinical Immunology1 / 3

    10 rows

    PolicyPayerEffective Date
    Diagnosis of Idiopathic Environmental IntoleranceOscar Health2026-10-01
    Allergen Testingblue cross blue shield - louisiana2026-09-15
    Allergen TestingBlue Cross Blue Shield - New Mexico2026-09-04
    Hereditary Angioedema - EkterlyCigna2026-08-15
    Omalizumab products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab Products (Xolair, Omlyclo) coveragePremera Bluecross2026-08-01
    Omalizumab ProductsPremera Bluecross2026-08-01
    Allergy Testinguniverahealthcare2026-07-16
    Omalizumab (Xolair) and related biologics coverageMass General Brigham Health Plan2026-07-01
    Xolair (omalizumab) (Subcutaneous) — Concomitant therapy restrictions and dosing guidanceeocco2026-07-01

    Related Codes

    86803-QUALITATIVE-OR-SEMIQUANTITATIVE-IMMUNOASSAYS-HEPATITIS-C

    CPT 86803: Hepatitis C Antibody Immunoassay in Serum

    CPT-86000-FEBRILE-AGGLUTININS-SEROLOGIC-TEST

    CPT 86000: Febrile Agglutinins (Serologic Agglutination) Test

    CPT-86001-ALLERGEN-SPECIFIC-IGG-LABORATORY-TEST

    CPT 86001: Allergen-Specific IgG Assay, Technical Component

    CPT-86003-ALLERGEN-SPECIFIC-IGE-ASSAY-TECHNICAL

    CPT 86003: Allergen‑Specific IgE Assay Using Crude Allergen Extract

    CPT-86005-MULTI-ALLERGEN-IGE-SCREENING-QUALITATIVE

    CPT 86005: Multi-Allergen Allergen-Specific IgE Screening

    CPT-86008-ALLERGEN-COMPONENT-SPECIFIC-IGE-ASSAY

    CPT 86008: Allergen Component–Specific IgE Assay

    CPT-86015-ANTI-ACTIN-SMOOTH-MUSCLE-ANTIBODY-ASMA

    CPT 86015: Actin (Smooth Muscle) Antibody (ASMA) Immunoassay

    CPT-86021-LEUKOCYTE-ANTIBODY-IDENTIFICATION-LAB-TECHNICAL

    CPT 86021: Leukocyte Antibody Identification Laboratory Test

    CPT-86022-PLATELET-ANTIBODY-IDENTIFICATION-TECHNICAL-TEST

    CPT 86022: Platelet Antibody Detection and Identification

    CPT-86023-PLATELET-ASSOCIATED-IMMUNOGLOBULIN-DETECTION

    CPT 86023: Platelet-Associated Immunoglobulin Assay

    Showing 1–10 of 199

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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