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    v1.0.28
    OpenPayerBilling CodesChemistry ProceduresCPT cpt-82658-enzymatic-activity-assay-radioactive-substrate
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    CPT 82658: Enzymatic Activity Assay Using Radioactive Substrate

    CPT code 82658 describes a laboratory enzymatic activity assay using a radioactive substrate to measure intracellular or tissue enzyme activity; this is a specialized enzymatic activity test performed by a lab analyst and is used only when the procedure is not otherwise listed. Service type: laboratory/clinical pathology. Typical site of service: clinical laboratory or hospital/laboratory tissue processing area.

    For related coverage guidance, see recent payer policy updates: Salivary Hormone Testing, Bone Turnover Markers Testing, Parathyroid Hormone, Phosphorus, Calcium, and Magnesium Testing.

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    Clinical & Coding Specifications

    Clinical Context

    A typical patient is a 45-year-old referred to the clinical laboratory by a hematology or metabolic specialist for investigation of suspected intracellular enzyme deficiency or for research-level enzymatic assays on tissue or cellular samples. The patient may present with unexplained muscle weakness, hepatosplenomegaly, neurodevelopmental delay, or metabolic crises prompting evaluation for disorders such as lysosomal storage disease, mitochondrial enzyme defects, or other inborn errors of metabolism. A tissue biopsy (e.g., liver, muscle) or isolated cells (e.g., leukocytes, fibroblasts) are collected in the clinic or outpatient surgical suite, transported to a specialized reference laboratory, and processed for cell disruption. The laboratory analyst performs an enzymatic activity assay using a radiolabeled substrate to quantify intracellular enzyme activity not covered by a listed CPT procedure. Results are interpreted by a clinical laboratory professional and reported to the ordering clinician for diagnostic confirmation, treatment planning, or further genetic testing. Typical site of service is an outpatient hospital-based pathology/laboratory or a specialized independent reference laboratory with biosafety capabilities.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    88305Level IV surgical pathology, gross and microscopic examinationTissue biopsy submitted for enzymatic assay may also undergo histopathologic evaluation billed with this code.
    83020Hemoglobin; glycohemoglobin (A1C)Routine metabolic monitoring tests that may be ordered concurrently in metabolic disease evaluation.
    87086Culture, bacterial; quantitative colony count, urineMicrobiology tests sometimes ordered on tissue or fluid samples as part of a broader diagnostic workup.
    81220CYP2C19 (e.g., genetic testing)Genetic testing frequently performed following abnormal enzymatic activity to identify causative mutations.
    0000FNot a CPT numeric code — Data not available in the input.

    Related Modifiers

    8 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207RG0100XGastroenterology PhysicianAn internist who specializes in diagnosis and treatment of diseases of the digestive organs including the stomach, bowels, liver and gallbladder. This…
    2080P0006XDevelopmental - Behavioral Pediatrics PhysicianA developmental-behavioral specialist is a pediatrician with special training and experience who aims to foster understanding and promotion of optimal…
    2086S0102XSurgical Critical Care PhysicianA surgeon with expertise in the management of the critically ill and postoperative patient, particularly the trauma victim, who specializes in critica…
    2084D0003XDiagnostic Neuroimaging (Psychiatry & Neurology) PhysicianA licensed physician, who has completed a residency program in Neurology, and who has additional training, experience, and competence in the standards…
    207QB0002XObesity Medicine (Family Medicine) PhysicianA physician who specializes in the treatment of obesity demonstrates competency in and a thorough understanding of the treatment of obesity and the ge…
    163WC1600XContinuing Education/Staff Development Registered NurseNursing Service Providers · Registered Nurse
    225500000XRespiratory/Developmental/Rehabilitative Specialist/TechnologistGeneral classification identifying individuals who are trained on a specific piece of equipment or technical procedure.
    156FX1201XOptometric Assistant TechnicianAn optometric assistant assists optometrists by performing duties, including but not limited to, customer service, basic eye testing, and patient educ…

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    C25.9Malignant neoplasm of pancreas, unspecified
    C34.90Malignant neoplasm of unspecified part of unspecified bronchus or lung
    C71.9Malignant neoplasm of brain, unspecified
    D50.9Iron deficiency anemia, unspecified
    D64.9Anemia, unspecified
    E70.0Classical phenylketonuria
    E71.0Maple-syrup-urine disease
    E72.9Disorder of amino-acid metabolism, unspecified
    E78.5Hyperlipidemia, unspecified
    E83.2Disorders of zinc metabolism

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: Endocrinology1 / 3

    5 rows

    PolicyPayerEffective Date
    Salivary Hormone TestingOscar Health2026-10-01
    Bone Turnover Markers TestingOscar Health2026-10-01
    Parathyroid Hormone, Phosphorus, Calcium, and Magnesium TestingOscar Health2026-10-01
    Testosterone — Coverage Criteria for Measurement and MonitoringOscar Health2026-10-01
    Thyroid Disease TestingOscar Health2026-10-01

    Related Codes

    CPT-82009-KETONE-BODIES-QUALITATIVE-TEST

    CPT 82009: Qualitative Ketone Bodies Test

    CPT-82010-QUANTITATIVE-KETONE-BODIES-ASSAY-SERUM-PLASMA

    CPT 82010: Quantitative Ketone Bodies in Serum or Plasma

    CPT-82013-ACETYLCHOLINESTERASE-QUANTITATIVE-ASSAY

    CPT 82013: Quantitative Acetylcholinesterase (Acetylhydrolase) Assay

    CPT-82016-QUALITATIVE-ACYLCARNITINES-METABOLIC-SCREEN

    CPT 82016: Qualitative Acylcarnitines, Plasma/Serum/Urine

    CPT-82017-QUANTITATIVE-ACYLCARNITINE-ANALYSIS

    CPT 82017: Quantitative Acylcarnitine Analysis

    CPT-82024-QUANTITATIVE-CORTICOTROPIN-ACTH-ASSAY

    CPT 82024: Adrenocorticotropic Hormone (ACTH) Quantitative Assay

    CPT-82030-QUANTITATIVE-CAMP-PLASMA-URINE-ASSAY

    CPT 82030: Quantitative cyclic AMP assay on plasma or urine

    CPT-82040-QUANTITATIVE-ALBUMIN-MEASUREMENT

    CPT 82040: Quantitative Albumin Measurement

    CPT-82042-QUANTITATIVE-ALBUMIN-NONBLOOD-FLUID

    CPT 82042: Albumin Quantitation in Non-Blood Body Fluid

    CPT-82043-URINE-ALBUMIN-QUANTITATION-KIDNEY-DISEASE

    CPT 82043: Urine Albumin Quantitative Assay

    Showing 1–10 of 411

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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