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    v1.0.28
    OpenPayerBilling CodesTherapeutic Drug AssaysCPT cpt-80333-serotonergic-antidepressant-assay-3-to-5-drugs
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    CPT 80333: Detection/Quantification of 3–5 Serotonergic Antidepressants

    CPT code 80333 describes a laboratory test that detects or quantifies three to five serotonergic antidepressant medications in a patient specimen; the service is a toxicology/pharmacology assay performed by a clinical laboratory, typically provided in an outpatient laboratory or hospital lab setting for monitoring therapeutic drug levels or assessing adherence and exposure to psychiatric medications.

    For related coverage guidance, see recent payer policy updates: Epithelial Cell Cytology in Breast Cancer Risk Assessment, Chemotherapy Observation or Inpatient Hospitalization (for New Mexico Only), Antibody-Drug Conjugates.

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    Clinical & Coding Specifications

    Clinical Context

    A 35-year-old outpatient with major depressive disorder managed on serotonergic agents presents for medication management after recent symptom changes and suspected nonadherence. The clinician orders therapeutic drug monitoring to measure serum concentrations of multiple serotonergic antidepressants to assess adherence, drug interactions, or unexpected levels. A phlebotomy technician collects a blood specimen at the outpatient laboratory or hospital outpatient service; the specimen is sent to the clinical laboratory. A laboratory analyst performs qualitative and quantitative testing that detects and/or measures three to five serotonergic antidepressant medications (for example, multiple selective serotonin reuptake inhibitors or serotonin–norepinephrine reuptake inhibitors) using methods such as liquid chromatography–mass spectrometry. Results are reported to the ordering psychiatrist, primary care provider, or pharmacy team. Typical sites of service include hospital outpatient laboratories, independent clinical reference laboratories, and ambulatory clinic laboratories. The clinical workflow includes order entry, specimen collection, chain-of-custody handling when required, laboratory analysis, result verification by a licensed laboratorian, and electronic results delivery to the ordering provider for clinical interpretation and follow-up.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80333Detection of three to five serotonergic antidepressants in a specimen; qualitative or quantitativePrimary code described: laboratory analysis detecting three to five serotonergic antidepressants in a patient specimen.
    80306Drug test(s), presumptive, any number of drug classes; any number of devices or procedures, per date of serviceOften performed as an initial point-of-care screen prior to definitive confirmatory testing like 80333.
    80307Drug test(s), presumptive, multiple drug classes, any number of devices or procedures, per date of serviceAnother presumptive screening code used in workflow before specific antidepressant panel confirmation.
    80305Drug test(s), presumptive, single class of drugUsed when a single-class rapid screen is performed and positive results lead to comprehensive testing such as 80333.
    82570Assay of drug, analgesic (e.g., acetaminophen)Example of other single-drug assays that may be ordered concurrently for toxicity evaluation when polypharmacy is a concern.
    80341Amphetamine(s), chromatographic, quantitativeRepresents other chromatographic confirmatory tests that may be performed in the laboratory alongside antidepressant panels for comprehensive toxicology.

    Related Modifiers

    7 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    207Q00000XFamily Medicine PhysicianFamily Medicine is the medical specialty which is concerned with the total health care of the individual and the family. It is the specialty in breadt…
    208000000XPediatrics PhysicianA pediatrician is concerned with the physical, emotional and social health of children from birth to young adulthood. Care encompasses a broad spectru…
    310400000XAssisted Living FacilityA facility providing supportive services to individuals who can function independently in most areas of activity, but need assistance and/or monitorin…
    207K00000XAllergy & Immunology PhysicianAn allergist-immunologist is trained in evaluation, physical and laboratory diagnosis, and management of disorders involving the immune system. Select…
    363LA2200XAdult Health Nurse PractitionerPhysician Assistants & Advanced Practice Nursing Providers · Nurse Practitioner
    3336N0007XNuclear PharmacyA pharmacy dedicated to the compounding and dispensing of radioactive materials for use in nuclear imaging and nuclear medical procedures.
    261QR0207XMobile Mammography Clinic/CenterAmbulatory Health Care Facilities · Clinic/Center
    225CA2500XAssistive Technology Supplier Rehabilitation CounselorRespiratory, Developmental, Rehabilitative and Restorative Service Providers · Rehabilitation Counselor

    Related ICD-10-CM Codes

    29 rows

    ICD-10-CM CodeDescription
    F06.32Mood disorder due to known physiological condition with major depressive-like episode
    F06.4Anxiety disorder due to known physiological condition
    F10.20Alcohol dependence, uncomplicated
    F11.20Opioid dependence, uncomplicated
    F19.20Other psychoactive substance dependence, uncomplicated
    F30.9Manic episode, unspecified
    F31.9Bipolar disorder, unspecified
    F32.0Major depressive disorder, single episode, mild
    F32.1Major depressive disorder, single episode, moderate
    F32.2Major depressive disorder, single episode, severe without psychotic features

    Showing 1–10 of 29

    Related Payer Policy Updates

    Specialty: Hematology/Oncology1 / 2

    4 rows

    PolicyPayerEffective Date
    Epithelial Cell Cytology in Breast Cancer Risk AssessmentOscar Health2026-10-01
    Chemotherapy Observation or Inpatient Hospitalization (for New Mexico Only)UnitedHealthcare2026-08-01
    Antibody-Drug ConjugatesPremera Bluecross2026-08-01
    Umbilical Cord Blood Harvesting and Storage for Future Use (for Indiana Only)UnitedHealthcare2026-08-01

    Related Codes

    CPT-80143-ACETAMINOPHEN-QUANTITATIVE-SERUM-ASSAY

    CPT 80143: Acetaminophen Quantitative Assay

    CPT-80145-ADALIMUMAB-LEVEL-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80145: Adalimumab Quantitative Drug Level Assay

    CPT-80150-AMIKACIN-QUANTITATIVE-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80150: Amikacin Quantitative Serum Level

    CPT-80151-AMIODARONE-QUANTITATIVE-SERUM-ASSAY

    CPT 80151: Amiodarone Quantitative Drug Level Assay

    CPT-80155-CAFFEINE-LEVEL-TESTING

    CPT 80155: Caffeine Level Quantification

    CPT-80156-CARBAMAZEPINE-TOTAL-LEVEL-LAB-ASSAY

    CPT 80156: Carbamazepine Total Level (Serum)

    CPT-80157-FREE-CARBAMAZEPINE-ASSAY

    CPT 80157: Free Carbamazepine Quantitative Assay

    CPT-80158-CYCLOSPORINE-QUANTITATIVE-ASSAY-LAB

    CPT 80158: Cyclosporine Quantitation, Laboratory

    CPT-80159-CLOZAPINE-LEVEL-ASSAY-THERAPEUTIC-DRUG-MONITORING

    CPT 80159: Clozapine Level Measurement

    CPT-80161-CARBAMAZEPINE-EPOXIDE-QUANTITATIVE-ASSAY

    CPT 80161: Carbamazepine 10,11-Epoxide Level, Quantitative

    Showing 1–10 of 87

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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