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    v1.0.30
    OpenPayerBilling CodesLaparoscopic Procedures on the Abdomen, Peritoneum, and OmentumCPT cpt-49321-diagnostic-laparoscopy-with-biopsy
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    CPT 49321: Diagnostic Laparoscopy with Biopsy

    Medicare pays $333 on average nationally for this procedure.

    CPT code 49321 describes a diagnostic laparoscopy with biopsy, where a surgeon uses a laparoscope to inspect the abdominal or pelvic cavity and obtains one or more tissue samples for laboratory diagnosis; service type: diagnostic surgical procedure; typical site of service: operating room or ambulatory surgery center with possible inpatient or outpatient setting depending on clinical context.

    For related coverage guidance, see recent payer policy updates: Surgical Procedures, Outpatient Surgical Procedures - Site of Service, Site of Service Ambulatory Service Center (ASC) Select — Surgical or Diagnostic Procedures in Adults.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old female presents with several weeks of lower abdominal pain, bloating, and an adnexal mass identified on ultrasound. Physical exam shows localized tenderness without peritoneal signs. After noninvasive imaging (pelvic ultrasound and CT) and laboratory evaluation including tumor markers, the gynecologic surgeon schedules a diagnostic laparoscopy with biopsy to evaluate the mass and obtain tissue for histopathology. The patient undergoes general anesthesia in an ambulatory surgical center. The surgeon establishes pneumoperitoneum, inserts a laparoscope, inspects the abdominal and pelvic organs, identifies a suspicious ovarian lesion, and performs targeted biopsies and peritoneal washings. Specimens are submitted to the pathology laboratory. The clinical workflow includes preoperative consent, anesthesia evaluation, sterile operative setup, laparoscopic inspection and biopsy, specimen labeling and documentation, immediate postoperative recovery, and discharge instructions with pathology follow-up.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    49320Laparoscopy, diagnostic, with or without collection of specimen(s) by brushing or washing (separate procedure)Nearly identical diagnostic laparoscopy code; may be used when only peritoneal washings or brushings are obtained rather than tissue biopsy
    49322Laparoscopy, surgical; with biopsy, single or multipleAlternative coding when biopsy is performed — 49321 describes diagnostic laparoscopy with biopsy; some payers recognize 49322 for more extensive laparoscopic biopsies or when therapeutic steps are taken
    49000Exploratory laparotomy, exploratory abdominal incisionOpen alternative when laparoscopy is converted to open procedure; used if procedure is converted intraoperatively
    49180Laparoscopy, surgical; enterolysis (freeing adhesions)May be performed if adhesions preventing visualization are lysed during diagnostic laparoscopy
    58100Endoscopic surgical procedures on the uterus (e.g., diagnostic hysteroscopy)May be performed in the same diagnostic episode for gynecologic evaluation when intrauterine assessment is indicated
    88305Surgical pathology, gross and microscopic examination, intermediate complexityTypical pathology CPT for evaluation of biopsy specimens obtained during laparoscopy; billed by the pathology provider as part of specimen analysis

    Related Modifiers

    12 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    23Modifier -23 (Unusual Anesthesia) is used on anesthesia service CPT codes when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia…- Use modifier -23 only on anesthesia CPT codes (00100–01999) when a procedure that normally requires no anesthesia or only local anesthesia must be performed under general anesthesia because of…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    50Modifier –50 (Bilateral Procedure) is appended to a CPT code when an identical procedure is performed on paired anatomical structures during the same operative session. The AMA states to add…- Use modifier -50 when the same CPT surgical, diagnostic, or radiology procedure is performed on paired anatomical structures (both sides of the body) during the same operative/session by the same…
    51Modifier –51 (Multiple Procedures) is used when the same provider performs multiple procedures in the same session to identify secondary, non‑exempt procedures and indicate payment reduction logic…- Use modifier -51 when the same provider performs multiple distinct procedures during the same patient encounter: report the highest‑valued procedure first (no modifier) and append -51 to…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…

    Showing 1–10 of 12

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    208800000X
    208600000X
    207R00000X
    208000000X
    202K00000X
    224Y00000X
    207XX0801X
    320700000X

    Related ICD-10-CM Codes

    23 rows

    ICD-10-CM CodeDescription
    C54.9Malignant neoplasm of corpus uteri, unspecified
    C56.9Malignant neoplasm of unspecified ovary
    K30Functional dyspepsia
    K35.80Unspecified acute appendicitis
    K52.9Noninfective gastroenteritis and colitis, unspecified
    K58.9Irritable bowel syndrome, unspecified
    K59.00Constipation, unspecified
    K86.1Other chronic pancreatitis
    N76.0Acute vaginitis
    N80.9Endometriosis, unspecified

    Showing 1–10 of 23

    Related Payer Policy Updates

    Specialty: General Surgery1 / 3

    10 rows

    PolicyPayerEffective Date
    Surgical ProceduresUnitedHealthcare2026-09-01
    Outpatient Surgical Procedures - Site of ServiceUnitedHealthcare2026-09-01
    Site of Service Ambulatory Service Center (ASC) Select — Surgical or Diagnostic Procedures in AdultsPremera Bluecross2026-09-01
    Site of Service Ambulatory Surgical Center (ASC) Select — Surgical or Diagnostic Procedures in AdultsPremera Bluecross2026-09-01
    Site of Service Ambulatory Service Center (ASC) SelectPremera Bluecross2026-09-01
    Site of Service Ambulatory Surgical Center (ASC) Select — Site of Service for Ambulatory Surgical Procedures (ASC Select)Premera Bluecross2026-09-01
    Site of Service Ambulatory Service Center (ASC) SelectPremera Bluecross2026-09-01
    Outpatient Surgical Procedures - Site of Servicesierra health and life2026-08-01
    Medical Necessity Guidelines — Inpatient Surgical CareMass General Brigham Health Plan2026-08-01
    Ambulatory Surgery Center (ASC) - Site of Care PolicyKaiser Permanente2026-08-01

    Related Codes

    CPT-49320-DIAGNOSTIC-LAPAROSCOPY-PERITONEUM-OMENTUM-INSPECTION

    CPT 49320: Diagnostic Laparoscopy of Peritoneum and Omentum

    CPT-49322-LAPAROSCOPIC-ASPIRATION-INTRA-ABDOMINAL-FLUID-CYSTS

    CPT 49322: Laparoscopic Aspiration of Abdominal Cyst Fluid

    CPT-49323-LAPAROSCOPIC-LYMPHOCELE-DRAINAGE

    CPT 49323: Laparoscopic Drainage of Lymphocele into Peritoneal Cavity

    CPT-49324-LAPAROSCOPIC-PERITONEAL-CATHETER-PLACEMENT-ASCITES-DRAINAGE

    CPT 49324: Laparoscopic Peritoneal Catheter Placement for Ascites

    CPT-49325-LAPAROSCOPIC-INTRAPERITONEAL-CATHETER-OBSTRUCTION-REMOVAL

    CPT 49325: Laparoscopic Removal of Intraperitoneal Catheter Obstruction

    CPT-49326-LAPAROSCOPIC-OMENTAL-TUCK-FOR-CATHETER-OCCLUSION

    CPT 49326: Laparoscopic Omental Tuck for Intraperitoneal Catheter Occlusion

    CPT-49327-LAPAROSCOPIC-FIDUCIAL-MARKER-PLACEMENT

    CPT 49327: Laparoscopic Placement of Fiducial Markers

    CPT-49329-LAPAROSCOPIC-ABDOMEN-PERITONEUM-OMENTAL-OTHERS

    CPT 49329: Laparoscopic Abdominal/Peritoneal/Omental Procedure, Unlisted

    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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