Summary & Overview
CPT 0581U: Autoantibody to Non–HLA Panel (Mayo Clinic Jacksonville)
CPT code 0581U denotes a Proprietary Laboratory Analyses (PLA) assay offered by Mayo Clinic Jacksonville that detects autoantibodies to non–HLA targets. This specialized blood test, using flow cytometry and single–antigen bead technology, screens for antibodies against 39 distinct non–HLA antigens that could influence transplant compatibility and outcomes. The code matters nationally as transplant programs and payers evaluate advanced immunologic risk profiling beyond HLA matching to inform patient management and post‑transplant monitoring.
Key payers in scope include Aetna, Blue Cross Blue Shield, Cigna Health, UnitedHealthcare, and Medicare. Readers will find a concise overview of the clinical application of the assay, the service setting, and the relevance of the PLA designation. The report summarizes typical payer coverage considerations, common billing modifiers reported for this code, and the clinical context linking non‑HLA antibodies to transplant risk assessment. It also highlights where input data were not provided and directs readers to reference lab documentation for technical details and manufacturer‑specific reporting.
Billing Code Overview
CPT code 0581U is a Proprietary Laboratory Analyses (PLA) test for Autoantibody to Non–Human Leukocyte Antigen (non–HLA) developed and performed by Mayo Clinic Jacksonville, Mayo Clinic. The assay analyzes a patient’s blood to identify antibodies that target antigens other than human leukocyte antigens, which may affect transplant outcomes. The test uses flow cytometry and single–antigen bead technology to evaluate reactivity against 39 distinct non–HLA targets and reports which specific antibodies are present.
Service Type: Specialty laboratory diagnostic testing (proprietary transplant immunology assay)
Typical Site of Service: Reference/Clinical laboratory
Clinical & Coding Specifications
Clinical Context
A 52-year-old patient undergoing evaluation for kidney transplantation at a tertiary transplant center has a pre-transplant immunology workup. The transplant team orders the Mayo Clinic Jacksonville proprietary assay 0581U to detect autoantibodies directed against 39 non–HLA targets that can influence graft outcomes. A peripheral blood sample is collected in the outpatient phlebotomy lab and sent to the performing laboratory. The test uses flow cytometry and single–antigen bead technology; results are reviewed by the transplant immunology service and incorporated into donor selection, risk stratification, and post‑transplant monitoring plans. Typical workflow steps include: order entry by transplant physician, specimen collection and labeling, courier transport to Mayo Clinic Jacksonville or designated performing lab, laboratory processing and reporting of detected non‑HLA antibodies, and multidisciplinary review by transplant surgery, nephrology, and transplant immunology.
Coding Specifications
| Modifier | Description | When to Use |
|---|---|---|
00 | No modifier | Default when no modifier applies to the service |
22 | Increased procedural services |