CPT code 0543U is a Proprietary Laboratory Analyses (PLA) code for the TruSight™ Oncology Comprehensive test from Illumina Inc., a next-generation sequencing tumor profiling assay that analyzes DNA from tumor tissue to detect single- and multi-nucleotide variants, insertions and deletions across 517 genes, and uses RNA to identify gene fusions and splice variants while calculating tumor mutation burden; service type: comprehensive genomic tumor profiling by next-generation sequencing (DNA and RNA) performed by a specialized clinical laboratory; typical site of service: outpatient or independent clinical molecular diagnostics laboratory processing surgical or biopsy tumor tissue.