CPT 0540U: AlloSure dd-cfDNA Test for Transplant Monitoring

CPT code 0540U is a Proprietary Laboratory Analyses (PLA) code for AlloSure® (CareDx®) that measures donor–derived cell–free DNA (dd–cfDNA) in plasma using next‑generation sequencing (NGS) to estimate the percentage of circulating DNA originating from a transplanted organ and help determine the probability of organ rejection; service type: laboratory dd‑cfDNA transplant surveillance test; typical site of service: outpatient or clinical laboratory drawing and analysis of plasma specimens.

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CPT 0540U: AlloSure dd-cfDNA Test for Transplant Monitoring

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Get alerted when payer policies referencing 0540U are released or updated.

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Clinical & Coding Specifications

Clinical Context

A 52-year-old patient with a history of kidney transplantation presents for routine post-transplant surveillance. The transplant nephrologist orders AlloSure® to measure donor-derived cell-free DNA (dd-cfDNA) from a peripheral blood draw to assess the probability of active allograft injury or rejection. A phlebotomy technician obtains a plasma specimen in the outpatient infusion clinic or laboratory. The specimen is sent to CareDx® Laboratory using validated collection and shipping procedures. CareDx® performs next-generation sequencing (NGS) analysis to quantify the percentage of dd-cfDNA. The laboratory report returns a dd-cfDNA percentage and interpretive guidance indicating whether levels are consistent with low probability of rejection or suggestive of potential rejection, prompting correlation with clinical assessment, serum creatinine, urinalysis, and possible indication for biopsy.

Typical site of service: outpatient hospital laboratory, independent reference laboratory, or outpatient transplant clinic. Service type: Proprietary Laboratory Analyses (PLA) — specialized molecular diagnostic assay for transplant monitoring.

Related CPT Codes

CPT Code	Description	Relationship to This Procedure
`0540U`	AlloSure® dd-cfDNA, quantitative NGS assay performed by CareDx® Laboratory (Proprietary Laboratory Analyses)	Primary PLA code used to report the AlloSure® assay for transplant rejection surveillance
`36415`	Collection of venous blood by venipuncture	Often performed immediately prior to sending plasma specimen for dd-cfDNA testing
`36416`	Collection of capillary blood specimen	Alternate collection method in select outpatient settings if validated by laboratory
`81002`	Urinalysis, non-automated, without microscopy	Common adjunctive test in transplant surveillance alongside dd-cfDNA to evaluate graft function
`82043`	Microalbumin; quantitative (e.g., urine)	May be performed concurrently as part of kidney transplant monitoring

Related Modifiers

5 rows

Modifier	Description	When to Use
22	Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…	- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
52	Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…	- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
53	Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…	- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
P4	Modifier P4 indicates the patient is ASA Physical Status IV — a patient with severe systemic disease that is a constant threat to life. It is assigned by the anesthesia provider and must be supported…	- When to apply: Use modifier P4 only on anesthesia CPT codes (00100–01999) to indicate ASA Physical Status IV — a patient with severe systemic disease that is a constant threat to life (examples:…
8P	Modifier 8P—Performance Measure Reporting Modifier – Action Not Performed, Reason Not Otherwise Specified— is appended only to CPT Category II quality-reporting codes to indicate that the specified…	- Use modifier -8P only with CPT Category II codes when the quality-measure action was not performed for an eligible patient and there is no documented reason in the medical record for omitting the…

Related Provider Taxonomies

8 rows

Taxonomy Code	Specialty	Notes
2080P0208X	Pediatric Infectious Diseases Physician	A pediatrician trained to care for children in the diagnosis, treatment and prevention of infectious diseases. This specialist can apply specific know…
207L00000X	Anesthesiology Physician	An anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
2084N0402X	Neurology with Special Qualifications in Child Neurology Physician	A Child Neurologist specializes in neurology with special skills in diagnosis and treatment of neurologic disorders of the neonatal period, infancy, e…
208D00000X	General Practice Physician	A physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
202D00000X	Integrative Medicine Physician	A physician who specializes in the treatment of the whole person through prevention and treatment based on medical evidence. Integrative medicine cons…
207RC0200X	Critical Care Medicine (Internal Medicine) Physician	An internist who diagnoses, treats and supports patients with multiple organ dysfunction. This specialist may have administrative responsibilities for…
163WU0100X	Urology Registered Nurse	Nursing Service Providers · Registered Nurse
152WP0200X	Pediatric Optometrist	Optometrists who work in Pediatrics are concerned with the prevention, development, diagnosis, and treatment of visual problems in children.

Related ICD-10-CM Codes

30 rows

ICD-10-CM Code	Description
A41.9	Sepsis, unspecified organism
B34.2	Coronavirus infection, unspecified
D63.8	Anemia in other chronic diseases classified elsewhere
D64.9	Anemia, unspecified
E10.21	Type 1 diabetes mellitus with diabetic nephropathy
E11.21	Type 2 diabetes mellitus with diabetic nephropathy
I12.9	Hypertensive chronic kidney disease with stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
I50.9	Heart failure, unspecified
J18.9	Pneumonia, unspecified organism
N18.5	Chronic kidney disease, stage 5

Showing 1–10 of 30

CPT 0001U: PreciseType HEA Erythrocyte Antigen Genotyping

CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

CPT 0002U: PolypDX™ Urine Metabolomic Risk Assay

CPT-0003U-OVAR-A-NEXT-GENERATION-OVARIAN-CANCER-RISK-ASSAY

CPT 0003U: Overa (MIA2G) Ovarian Cancer Risk Assay

CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

CPT 0005U: ExosomeDx Prostate (IntelliScore) urine gene expression test

CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

CPT 0007U: ToxProtect Urine Drug Screen With DNA Authentication

CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

CPT 0008U: H. pylori Antibiotic Resistance Gene Panel

CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

CPT 0011U: Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcripts

Showing 1–10 of 536

References

1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.

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Trek Health ingests and normalizes Transparency in Coverage data and payer policy updates to give provider organizations a clear view of how commercial reimbursement behaves across markets, payers, and services. Our platform transforms raw payer disclosures into structured intelligence that supports contract evaluation, payer negotiations, and service line strategy. By combining market benchmarks with ongoing policy visibility, Trek helps teams identify variability, risk, and opportunity in commercial reimbursement. The result is faster insight, stronger negotiating positions, and more informed financial decisions.

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Clinical & Coding Specifications

Clinical Context

Related CPT Codes

CPT Code	Description	Relationship to This Procedure
`0540U`	AlloSure® dd-cfDNA, quantitative NGS assay performed by CareDx® Laboratory (Proprietary Laboratory Analyses)	Primary PLA code used to report the AlloSure® assay for transplant rejection surveillance
`36415`	Collection of venous blood by venipuncture	Often performed immediately prior to sending plasma specimen for dd-cfDNA testing
`36416`	Collection of capillary blood specimen	Alternate collection method in select outpatient settings if validated by laboratory
`81002`	Urinalysis, non-automated, without microscopy	Common adjunctive test in transplant surveillance alongside dd-cfDNA to evaluate graft function
`82043`	Microalbumin; quantitative (e.g., urine)	May be performed concurrently as part of kidney transplant monitoring

Related Modifiers

5 rows

Modifier	Description	When to Use
22	Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…	- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
52	Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…	- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
53	Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…	- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
P4	Modifier P4 indicates the patient is ASA Physical Status IV — a patient with severe systemic disease that is a constant threat to life. It is assigned by the anesthesia provider and must be supported…	- When to apply: Use modifier P4 only on anesthesia CPT codes (00100–01999) to indicate ASA Physical Status IV — a patient with severe systemic disease that is a constant threat to life (examples:…
8P	Modifier 8P—Performance Measure Reporting Modifier – Action Not Performed, Reason Not Otherwise Specified— is appended only to CPT Category II quality-reporting codes to indicate that the specified…	- Use modifier -8P only with CPT Category II codes when the quality-measure action was not performed for an eligible patient and there is no documented reason in the medical record for omitting the…

Related Provider Taxonomies

8 rows

Taxonomy Code	Specialty	Notes
2080P0208X	Pediatric Infectious Diseases Physician	A pediatrician trained to care for children in the diagnosis, treatment and prevention of infectious diseases. This specialist can apply specific know…
207L00000X	Anesthesiology Physician	An anesthesiologist is trained to provide pain relief and maintenance, or restoration, of a stable condition during and immediately following an opera…
2084N0402X	Neurology with Special Qualifications in Child Neurology Physician	A Child Neurologist specializes in neurology with special skills in diagnosis and treatment of neurologic disorders of the neonatal period, infancy, e…
208D00000X	General Practice Physician	A physician who specializes in the general practice of diagnosing, treating, and managing patients with a variety of illnesses and conditions.
202D00000X	Integrative Medicine Physician	A physician who specializes in the treatment of the whole person through prevention and treatment based on medical evidence. Integrative medicine cons…
207RC0200X	Critical Care Medicine (Internal Medicine) Physician	An internist who diagnoses, treats and supports patients with multiple organ dysfunction. This specialist may have administrative responsibilities for…
163WU0100X	Urology Registered Nurse	Nursing Service Providers · Registered Nurse
152WP0200X	Pediatric Optometrist	Optometrists who work in Pediatrics are concerned with the prevention, development, diagnosis, and treatment of visual problems in children.

Related ICD-10-CM Codes

30 rows

ICD-10-CM Code	Description
A41.9	Sepsis, unspecified organism
B34.2	Coronavirus infection, unspecified
D63.8	Anemia in other chronic diseases classified elsewhere
D64.9	Anemia, unspecified
E10.21	Type 1 diabetes mellitus with diabetic nephropathy
E11.21	Type 2 diabetes mellitus with diabetic nephropathy
I12.9	Hypertensive chronic kidney disease with stage 1 through stage 4 chronic kidney disease, or unspecified chronic kidney disease
I50.9	Heart failure, unspecified
J18.9	Pneumonia, unspecified organism
N18.5	Chronic kidney disease, stage 5

Showing 1–10 of 30

CPT 0001U: PreciseType HEA Erythrocyte Antigen Genotyping

CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

CPT 0002U: PolypDX™ Urine Metabolomic Risk Assay

CPT-0003U-OVAR-A-NEXT-GENERATION-OVARIAN-CANCER-RISK-ASSAY

CPT 0003U: Overa (MIA2G) Ovarian Cancer Risk Assay

CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

CPT 0005U: ExosomeDx Prostate (IntelliScore) urine gene expression test

CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

CPT 0007U: ToxProtect Urine Drug Screen With DNA Authentication

CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

CPT 0008U: H. pylori Antibiotic Resistance Gene Panel

CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

CPT 0011U: Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcripts

Showing 1–10 of 536

References

1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.

Published by

Trek Health powers OpenPayer with a leading commercial payer intelligence platform, seamlessly converting fragmented payer data into actionable insights for provider organizations.

OpenPayer

CPT 0540U: AlloSure dd-cfDNA Test for Transplant Monitoring

Sign up for cpt 0540U policy alerts

OpenPayer

CPT 0540U: AlloSure dd-cfDNA Test for Transplant Monitoring

Sign up for cpt 0540U policy alerts

Clinical & Coding Specifications

Clinical Context

Related CPT Codes

Related Modifiers

Related Provider Taxonomies

Related ICD-10-CM Codes

Related Codes

References

Clinical & Coding Specifications

Clinical Context

Related CPT Codes

Related Modifiers

Related Provider Taxonomies

Related ICD-10-CM Codes

Related Codes

References