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    OpenPayerBilling CodesProprietary Laboratory AnalysesCPT cpt-0254u-smart-pgta-embryo-aneuploidy-ngs
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    CPT 0254U: SMART PGT–A (Preimplantation Genetic Testing – Aneuploidy)

    CPT code 0254U is a Proprietary Laboratory Analyses (PLA) code for the SMART PGT–A (Preimplantation Genetic Testing – Aneuploidy) assay from Igenomix® USA that uses next generation sequencing (NGS) and mitochondrial DNA scoring to evaluate an embryo biopsy for chromosomal abnormalities (aneuploidy); service type: genetic diagnostic testing for embryo selection during in vitro fertilization (IVF); typical site of service: fertility clinic or specialized reproductive genetics laboratory performing embryo biopsy and molecular analysis.

    For related coverage guidance, see recent payer policy updates: Prenatal (Fetal) Diagnostic Testing, Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency.

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    Clinical & Coding Specifications

    Clinical Context

    A 36-year-old woman undergoing in vitro fertilization (IVF) elects preimplantation genetic testing for aneuploidy (PGT‑A) using the SMART PGT‑A assay from Igenomix® USA to improve embryo selection. During IVF, mature oocytes are retrieved, fertilized (conventional IVF or ICSI), and embryos are cultured to blastocyst stage (day 5–7). A trophectoderm biopsy (typically 3–10 cells) is performed by the embryology team in the IVF laboratory and the sample is prepared for transport to the proprietary laboratory performing the SMART PGT‑A test. The assay uses next generation sequencing and mitochondrial DNA scoring to detect chromosomal aneuploidies and provide mitochondrial DNA metrics that may inform embryo viability.

    The clinical workflow includes pre‑test counseling and consent, coordinated scheduling of biopsy on the embryo culture day, sample labeling and chain‑of‑custody documentation, specimen transport to the performing lab, laboratory processing with NGS and mtDNA analysis, and reporting of results back to the reproductive endocrinologist and patient to guide embryo transfer decisions. Typical site of service is an outpatient IVF clinic with embryo biopsy performed in the embryology laboratory; analytic testing occurs at the proprietary reference laboratory.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    0254USMART PGT–A (Preimplantation Genetic Testing – Aneuploidy) assay using NGS and mtDNA scoringThe proprietary PLA code for the specific laboratory test performed on an embryo biopsy specimen
    88381Morphometric analysis, microscopically; special staining, each specimenMay be used in embryology labs for formal microscopic evaluation of biopsy specimens if billed separately
    89250Oocyte retrieval, follicular puncturePerformed earlier in the IVF cycle to obtain oocytes that will be fertilized and produce embryos for PGT‑A
    89251Embryo biopsy, not otherwise specified (note: specific embryo biopsy codes vary)Procedure code representing embryo biopsy performed in the embryology lab prior to sending specimen for PGT‑A
    89260Assisted reproductive technology; embryo culture and transferRepresents embryo culture and transfer services that occur after PGT‑A results guide selection

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    54Modifier ‑54 (“Surgical care only”) is appended to a CPT surgical procedure when the reporting surgeon performs the intraoperative (surgical) component but does not furnish the preoperative and/or…- Use modifier -54 when the reporting surgeon provided only the intraoperative (surgical) component of a procedure and preoperative and/or postoperative management has been or will be provided by…
    55Modifier –55 indicates the billing provider furnished only postoperative management for a procedure that has a global surgical period. According to AAPC, this modifier is appended to the same CPT…- Use modifier -55 when billing the same CPT surgical code that has a 10- or 90-day global period and you, as a provider other than the original surgeon, furnished ONLY postoperative management (no…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    78CPT modifier -78 indicates an unplanned, related return to the operating or procedure room by the same provider during the global postoperative period. It is appended when treatment for a…- Use modifier -78 when an unplanned, related complication during the original procedure’s global postoperative period requires the patient to return to a formal operating or procedure room and the…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    174400000X
    207K00000X
    174H00000X
    208000000X
    363L00000X
    2251X0800X
    111NN1001X
    246QB0000X

    Related ICD-10-CM Codes

    21 rows

    ICD-10-CM CodeDescription
    E28.2Polycystic ovarian syndrome
    F53.0Postpartum depression
    N72Inflammatory disease of cervix uteri
    N97.0Female infertility associated with anovulation
    N97.1Female infertility of tubal origin
    N97.2Female infertility of uterine origin
    N97.8Female infertility of other origin
    N97.9Female infertility, unspecified
    O09.611Supervision of young primigravida, first trimester
    O09.612Supervision of young primigravida, second trimester

    Showing 1–10 of 21

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Prenatal (Fetal) Diagnostic TestingBlue Cross Blue Shield - Rhode Island2027-01-01
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Polygenic Risk ScoreBlue Cross Blue Shield - Rhode Island2026-12-01
    Gene Expression Profiling for Cutaneous MelanomaBlue Cross Blue Shield - Rhode Island2026-12-01
    Genetic Testing for Diagnosis and Management of Mental Health ConditionsBlue Cross Blue Shield - Rhode Island2026-11-01
    Molecular Testing in the Management of Pulmonary NodulesBlue Cross Blue Shield - Rhode Island2026-11-01
    Genetic Testing for Hereditary Cancer (for North Carolina Only)Colorado Rocky Mountain Health Plans2026-10-01
    Expanded Molecular Testing of Cancers to Select Targeted TherapiesBlue Cross Blue Shield - North Dakota2026-10-01

    Related Codes

    CPT-0001U-PRECISETYPE-HEA-MULTIPLEX-RBC-ANTIGEN-GENOTYPING

    CPT 0001U: PreciseType HEA Erythrocyte Antigen Genotyping

    CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

    CPT 0002U: PolypDX™ Urine Metabolomic Risk Assay

    CPT-0003U-OVAR-A-NEXT-GENERATION-OVARIAN-CANCER-RISK-ASSAY

    CPT 0003U: Overa (MIA2G) Ovarian Cancer Risk Assay

    CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

    CPT 0005U: ExosomeDx Prostate (IntelliScore) urine gene expression test

    CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

    CPT 0007U: ToxProtect Urine Drug Screen With DNA Authentication

    CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

    CPT 0008U: H. pylori Antibiotic Resistance Gene Panel

    CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

    CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

    CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

    CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

    CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

    CPT 0011U: Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

    CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

    CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcripts

    Showing 1–10 of 536

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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