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    v1.0.30
    OpenPayerBilling CodesProprietary Laboratory AnalysesCPT cpt-0130u-rnainsight-colon-cancer-mrna-panel
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    CPT 0130U: +RNAinsight™ for ColoNext® targeted mRNA 10-gene panel

    CPT code 0130U is a Proprietary Laboratory Analyses (PLA) code for the +RNAinsight™ for ColoNext® test from Ambry Genetics, a targeted mRNA sequence analysis panel of 10 genes used to improve variant classification for hereditary colon cancer disorders; service type: targeted mRNA sequence analysis (molecular diagnostic laboratory test); typical site of service: independent or hospital-based clinical molecular laboratory or reference laboratory performing specialized genetic testing.

    For related coverage guidance, see recent payer policy updates: Prenatal (Fetal) Diagnostic Testing, Oral Cancer Screening and Testing, RTM Testing for Alpha-1 Antitrypsin Deficiency.

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    Table of contents

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    Clinical & Coding Specifications

    Clinical Context

    A 42-year-old patient with a family history of colorectal cancer and a prior identification of a variant of uncertain significance (VUS) in a colon cancer–associated gene is referred for further molecular characterization. The clinician orders the Ambry Genetics +RNAinsight™ for ColoNext® test to evaluate RNA splicing and transcript effects across a targeted 10-gene mRNA panel to improve variant classification for hereditary colorectal cancer syndromes. Specimen collection is typically a peripheral blood draw or a properly preserved blood tube shipped to the performing laboratory. The laboratory performs targeted mRNA extraction, reverse transcription, and sequence analysis to detect aberrant splicing or expression changes related to DNA variants identified by prior germline testing. Results are reported to the ordering genetics provider or genetic counselor, who integrates the RNA findings with prior DNA test results, family history, and clinical data to inform variant reclassification and management decisions. Typical sites of service include outpatient genetic counseling clinics, specialty oncology/genetics clinics, and reference laboratory facilities. The service is billed as an add-on Proprietary Laboratory Analysis using 0130U in addition to the primary DNA/genetic testing CPT code.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    0130URNAinsight™ for ColoNext® (Ambry Genetics) — targeted mRNA sequence analysis panel of 10 genes (PLA add-on)The primary Proprietary Laboratory Analysis code describing the specific RNA assay; reported in addition to the primary genetic test CPT when applicable.
    81211BRCA1 gene analysis, full sequence analysis (example of hereditary cancer DNA testing)DNA sequence analysis codes like this represent the primary germline testing that often precedes RNA analysis; 0130U is reported in addition when RNA testing is performed to clarify DNA variants.
    81479Unlisted molecular pathology procedureUsed when a payer requires a general molecular pathology unlisted code as part of the testing bundle or when reporting services not otherwise specified alongside PLA reporting.
    88369Microdissection, manual (for molecular assays requiring tumor enrichment)May be performed before molecular testing if tissue-based enrichment is needed prior to RNA extraction in tissue specimens, supporting downstream RNA analysis.
    80502CYP2C19 genotype, 2 or 3 variants (example pharmacogenomic test)Represents other specialized molecular tests that may be performed in the same laboratory workflow; included here as commonly co-billed molecular assays.
    99000Handling and/or conveyance of specimen (example administrative code)Administrative/handling codes may be appended per payer rules for specimen collection, preparation, or shipping related to specialized laboratory testing.

    Related Modifiers

    9 rows

    ModifierDescriptionWhen to Use
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    62Modifier 62 indicates that two surgeons function as co‑surgeons, each performing distinct parts of the same primary surgical procedure. It is appended to the primary procedure code (and any…- Use modifier -62 when two surgeons act as co‑surgeons (both primary surgeons) on a single definitive primary procedure, each performing distinct parts of the same surgical procedure (append -62 to…
    76Modifier -76, Repeat Procedure or Service by Same Physician or Other Qualified Health Care Professional, is appended when the same provider performs an identical, medically necessary procedure or…- Use modifier -76 when the same physician or other qualified health care professional performs an identical procedure or service a second (or subsequent) time on the same date of service and the…
    77Modifier 77 indicates that a previously performed procedure or service was legitimately repeated by a different physician or other qualified health care professional. The AMA’s CPT definition…- Use modifier 77 when a basic procedure or service already performed is repeated by a different physician or other qualified health care professional (for example, a second provider independently…
    80CPT® Modifier 80 (Assistant Surgeon) is appended to a surgical CPT code when a physician (MD or DO) provides full, continuous assistance throughout the operative procedure. It is intended only for…- Use CPT Modifier -80 when a physician (MD or DO) serves as an assistant surgeon and provides full, continuous assistance throughout the entire operative procedure; append -80 to the surgical CPT…
    82Modifier 82 is appended to a surgical CPT code to indicate that an attending physician acted as an assistant because a qualified resident surgeon was unavailable. In teaching hospitals it documents…- When to use: Append modifier 82 in teaching-hospital settings to indicate a physician served as an assistant surgeon because a qualified resident surgeon was unavailable (includes situations where…

    Related Provider Taxonomies

    8 rows

    Taxonomy Code
    207Q00000X
    363L00000X
    207K00000X
    207L00000X
    225XH1200X
    225400000X
    286500000X
    261QR1100X

    Related ICD-10-CM Codes

    21 rows

    ICD-10-CM CodeDescription
    C18.9Malignant neoplasm of colon, unspecified
    C20Malignant neoplasm of rectum
    D12.6Benign neoplasm of colon, unspecified
    D37.4Neoplasm of uncertain behavior of colon
    D50.9Iron deficiency anemia, unspecified
    K57.30Diverticulosis of large intestine without perforation or abscess without bleeding
    K57.50Diverticulosis of both small and large intestine without perforation or abscess without bleeding
    K63.5Polyp of colon
    K92.2Gastrointestinal hemorrhage, unspecified
    R19.4Change in bowel habit

    Showing 1–10 of 21

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    Prenatal (Fetal) Diagnostic TestingBlue Cross Blue Shield - Rhode Island2027-01-01
    Oral Cancer Screening and TestingOscar Health2027-01-01
    RTM Testing for Alpha-1 Antitrypsin DeficiencyOscar Health2027-01-01
    Cytochrome P450 Genotype-Guided Treatment Strategy Corporate Medical Policybcbsvermont2027-01-01
    Polygenic Risk ScoreBlue Cross Blue Shield - Rhode Island2026-12-01
    Gene Expression Profiling for Cutaneous MelanomaBlue Cross Blue Shield - Rhode Island2026-12-01
    Genetic Testing for Diagnosis and Management of Mental Health ConditionsBlue Cross Blue Shield - Rhode Island2026-11-01
    Molecular Testing in the Management of Pulmonary NodulesBlue Cross Blue Shield - Rhode Island2026-11-01
    Genetic Testing for Hereditary Cancer (for North Carolina Only)Colorado Rocky Mountain Health Plans2026-10-01
    Expanded Molecular Testing of Cancers to Select Targeted TherapiesBlue Cross Blue Shield - North Dakota2026-10-01

    Related Codes

    CPT-0001U-PRECISETYPE-HEA-MULTIPLEX-RBC-ANTIGEN-GENOTYPING

    CPT 0001U: PreciseType HEA Erythrocyte Antigen Genotyping

    CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

    CPT 0002U: PolypDX™ Urine Metabolomic Risk Assay

    CPT-0003U-OVAR-A-NEXT-GENERATION-OVARIAN-CANCER-RISK-ASSAY

    CPT 0003U: Overa (MIA2G) Ovarian Cancer Risk Assay

    CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

    CPT 0005U: ExosomeDx Prostate (IntelliScore) urine gene expression test

    CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

    CPT 0007U: ToxProtect Urine Drug Screen With DNA Authentication

    CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

    CPT 0008U: H. pylori Antibiotic Resistance Gene Panel

    CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

    CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

    CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

    CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

    CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

    CPT 0011U: Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

    CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

    CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcripts

    Showing 1–10 of 536

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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