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    v1.0.28
    OpenPayerBilling CodesProprietary Laboratory AnalysesCPT cpt-0077u-m-protein-detection-isotyping-maldi-tof
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    CPT 0077U: M–Protein Detection and Isotyping by MALDI–TOF Mass Spectrometry

    CPT code 0077U is a Proprietary Laboratory Analyses (PLA) code for M–Protein Detection and Isotyping by MALDI–TOF Mass Spectrometry, a laboratory diagnostic test developed by the Mayo Clinic Laboratory to isolate and identify monoclonal (M–) proteins in a patient specimen to aid in diagnosing monoclonal gammopathy; service type: specialized clinical laboratory diagnostic testing using mass spectrometry; typical site of service: outpatient or hospital clinical laboratory or reference laboratory.

    For related coverage guidance, see recent payer policy updates: RTM Testing of Homocysteine Metabolism-Related Conditions, Multimarker Serum Testing Related to Ovarian Cancer, Genomic Profiling for Selecting Targeted Cancer Therapies.

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    Table of contents

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    • ModifiersCPT modifiers & usage
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    Clinical & Coding Specifications

    Clinical Context

    A 67-year-old male with unexplained anemia and hypercalcemia is referred by his hematologist for laboratory evaluation of a suspected monoclonal gammopathy. The patient presents for routine phlebotomy at an outpatient hospital laboratory. The ordering clinician requests M–Protein Detection and Isotyping by MALDI–TOF Mass Spectrometry, reported under code 0077U, to isolate and identify monoclonal (M–) proteins in the serum sample to aid in diagnosis and isotype classification (for example, IgG kappa, IgA lambda). The clinical workflow: the clinician orders the test in the electronic medical record, the specimen (serum) is collected, labeled, and sent to the Mayo Clinic Laboratory or the performing proprietary laboratory. The specimen is processed and analyzed using MALDI–TOF mass spectrometry. Results returned to the ordering clinician include detection of an M–protein and its isotype, which informs further diagnostic steps such as bone marrow biopsy, serum immunofixation, serum free light chain testing, imaging, or hematology referral. Typical site of service is an outpatient clinical laboratory or hospital laboratory with referral to the proprietary testing laboratory when required. The service type is a specialized laboratory diagnostic test (Proprietary Laboratory Analysis) performed on blood/serum for protein characterization in suspected monoclonal gammopathy.

    Related CPT Codes

    CPT CodeDescriptionRelationship to This Procedure
    80055General health panel; includes calcium and other chemistriesPerformed before or alongside to evaluate hypercalcemia and renal function in suspected monoclonal gammopathy.
    84165Immunoglobulin quantification; serumProvides quantitative immunoglobulin levels (IgG, IgA, IgM) that complement M–protein detection and isotyping.
    86336Immunoassay for infectious agent antibody(ies) or antigen(s), qualitative or semiquantitative, single stepRepresents standard immunoassay methods; may be performed in parallel laboratories for differential testing (note: used generically for related serologic assays).
    84402Thyroid stimulating hormone (TSH)Example of common concurrent laboratory testing in broad diagnostic workups of older adults (clinical workflow adjunct).
    85025Complete blood count (CBC) with automated differentialFrequently ordered to evaluate anemia and other hematologic abnormalities that prompt monoclonal protein testing.

    Related Modifiers

    7 rows

    ModifierDescriptionWhen to Use
    90Modifier ‑90 (Reference/Outside Laboratory) indicates that a laboratory test was performed by an entity other than the treating or reporting provider. The American Medical Association defines…- Use CPT modifier -90 when a laboratory test is performed by an outside/reference laboratory (an entity other than the treating or reporting physician or qualified professional) and the claim needs…
    91Modifier 91 — “Repeat Clinical Diagnostic Laboratory Test” — is appended when the exact same CPT laboratory test is performed more than once for the same patient on the same day because serial,…- Use modifier 91 when the exact same CPT laboratory test is performed more than once on the same patient on the same date because serial or repeated testing is medically necessary to monitor…
    26Modifier 26 (Professional Component) identifies and bills only the professional portion of a split service — the physician’s interpretation, report, and medical judgment — when the technical…- Use CPT Modifier -26 when you are billing only the professional component (PC) of a split‑bill eligible service—i.e., you performed the physician interpretation, medical judgment and prepared a…
    59Modifier 59 indicates a distinct procedural service when two procedures that are not normally billed together are clinically and procedurally separate. The CPT definition (as reiterated by CMS)…- Use modifier -59 to report a distinct procedural service when documentation supports that the procedure reported is not normally billed together with another service on the same day because of a…
    52Modifier ‑52 (Reduced Services) indicates that a service or procedure was partially reduced or omitted at the provider’s discretion, allowing reporting of the basic procedure number with a qualifier…- Use modifier -52 when a physician or qualified health professional electively reduces or omits part of a procedure or service (for example, performing fewer required views or omitting a portion of…
    53Modifier 53 (Discontinued Procedure) signals that a surgical or diagnostic service was begun by the physician but terminated because of extenuating circumstances that threatened the patient’s…- Use modifier -53 when a physician/qualified healthcare professional has started a surgical or diagnostic procedure (e.g., anesthesia administered, incision or scope insertion) and then discontinues…
    22Modifier –22 (Increased Procedural Services) is used when a reported procedure is substantially more extensive than normally required. The CPT Manual and CMS state it may be appended only when the…- When to use: Append modifier -22 to a procedural CPT code when the service performed is substantially more extensive than the usual service described by that code — i.e., the procedure required…

    Related Provider Taxonomies

    8 rows

    Taxonomy CodeSpecialtyNotes
    2080P0206XPediatric Gastroenterology PhysicianA pediatrician who specializes in the diagnosis and treatment of diseases of the digestive systems of infants, children and adolescents. This speciali…
    207RP1001XPulmonary Disease PhysicianAn internist who treats diseases of the lungs and airways. The pulmonologist diagnoses and treats cancer, pneumonia, pleurisy, asthma, occupational an…
    208000000XPediatrics PhysicianA pediatrician is concerned with the physical, emotional and social health of children from birth to young adulthood. Care encompasses a broad spectru…
    363L00000XNurse Practitioner(1) A registered nurse provider with a graduate degree in nursing prepared for advanced practice involving independent and interdependent decision mak…
    103TE1000XDeactivated - Psychologist
    207X00000XOrthopaedic Surgery PhysicianAn orthopaedic surgeon is trained in the preservation, investigation and restoration of the form and function of the extremities, spine and associated…
    163WI0600XInfection Control Registered NurseNursing Service Providers · Registered Nurse
    261QE0700XEnd-Stage Renal Disease (ESRD) Treatment Clinic/CenterAmbulatory Health Care Facilities · Clinic/Center

    Related ICD-10-CM Codes

    26 rows

    ICD-10-CM CodeDescription
    B20Human immunodeficiency virus [HIV] disease
    C90.00Multiple myeloma not having achieved remission
    C90.02Multiple myeloma in relapse
    C90.10Plasma cell leukemia not having achieved remission
    C90.12Plasma cell leukemia in relapse
    C90.20Extramedullary plasmacytoma not having achieved remission
    C90.22Extramedullary plasmacytoma in relapse
    D47.1Chronic myeloproliferative disease
    D47.2Monoclonal gammopathy
    D47.9Neoplasm of uncertain behavior of lymphoid, hematopoietic and related tissue, unspecified

    Showing 1–10 of 26

    Related Payer Policy Updates

    Specialty: Genetic & Molecular Testing1 / 3

    10 rows

    PolicyPayerEffective Date
    RTM Testing of Homocysteine Metabolism-Related ConditionsOscar Health2026-10-01
    Multimarker Serum Testing Related to Ovarian CancerBlue Cross Blue Shield - Rhode Island2026-09-01
    Genomic Profiling for Selecting Targeted Cancer TherapiesBlue Cross Blue Shield - Rhode Island2026-09-01
    Whole Exome and Whole Genome Sequencing for Diagnosis of Genetic DisordersBlue Cross Blue Shield - Rhode Island2026-09-01
    Biomarker and Genetic/Molecular Testing Coverage and Prior AuthorizationBlue Cross Blue Shield - Rhode Island2026-09-01
    Non-Invasive Prenatal Testing (NIPT)univerahealthcare2026-08-17
    Lumizyme (alglucosidase alfa) — Pompe Disease (acid alpha‑glucosidase deficiency)Cigna2026-08-15
    Molecular Oncology Testing for Solid Tumor Cancer Diagnosis, Prognosis, and Treatment DecisionsColorado Rocky Mountain Health Plans2026-08-01
    Carrier Testing Panels for Genetic DiseasesColorado Rocky Mountain Health Plans2026-08-01
    Molecular Oncology Testing for Hematologic Cancer Diagnosis, Prognosis, and Treatment DecisionsColorado Rocky Mountain Health Plans2026-08-01

    Related Codes

    CPT-0001U-PRECISETYPE-HEA-MULTIPLEX-RBC-ANTIGEN-GENOTYPING

    CPT 0001U: PreciseType HEA Erythrocyte Antigen Genotyping

    CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

    CPT 0002U: PolypDX™ Urine Metabolomic Risk Assay

    CPT-0003U-OVAR-A-NEXT-GENERATION-OVARIAN-CANCER-RISK-ASSAY

    CPT 0003U: Overa (MIA2G) Ovarian Cancer Risk Assay

    CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

    CPT 0005U: ExosomeDx Prostate (IntelliScore) urine gene expression test

    CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

    CPT 0007U: ToxProtect Urine Drug Screen With DNA Authentication

    CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

    CPT 0008U: H. pylori Antibiotic Resistance Gene Panel

    CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

    CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

    CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

    CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

    CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

    CPT 0011U: Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

    CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

    CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcripts

    Showing 1–10 of 536

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    Frequently Asked Questions

    References

    1. 1.CPT® Codes and Descriptions. AAPC. Retrieved Aug 11, 2026.Maintains the CPT code set's official descriptions and guidelines, licensed from the American Medical Association, used as the definitional source for CPT codes on this page.
    2. 2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's quarterly release of HCPCS Level II codes and short/long descriptors, the source for HCPCS code definitions and update history.
    3. 3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Aug 11, 2026.CMS's complete ICD-10-CM diagnosis code list, used to cross-reference diagnosis codes associated with this procedure or service.
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